Tag: M.W=450-500

Higuchi, Masahiro published the artcileModulation of two forms of tumor necrosis factor receptors and their cellular response by soluble receptors and their monoclonal antibodies, Formula: C18H20N2O12, the publication is Journal of Biological Chemistry (1992), 267(29), 20892-9, database is CAplus and MEDLINE.

Recently, two different receptors for human tumor necrosis factor (TNF) with mol. masses of 60 kDa (p60) and 80 kDa (p80) have been identified. This report investigated the effect of the soluble forms of these receptors and monoclonal antibodies against them on ligand interaction, receptor down-regulation, and mediation of cellular response in U-937 cells. The results indicate that p60 and p80 constitute 20-30 and 60-80% of the total TNF-binding sites on U-937 cells, resp. However, by crosslinking, only the p80 form of the receptor could be detected. In contrast to unlabeled TNF, the anti-p60 and anti-p80 antibodies together only partially inhibited ligand binding, and this inhibition was not additive. Lack of additive inhibition of binding was not due to stereochem. hindrance. TNF binding to cells can be completely displaced by soluble forms of either the p60 or p80 receptor. However, 100-fold more of the p80 than the p60 form of the soluble receptor is needed for equivalent displacement. Under optimum conditions, TNF and the anti-p80 and anti-p60 antibodies down-regulated 30, 80, and 20% of the TNF receptors, resp. The anti-p60 and anti-p80 antibodies down-regulated not only their own receptors, but also reciprocal receptors, suggesting a cross-communication between the p60 and p80 forms of the TNF receptor. In spite of inhibiting as much as 80% of TNF binding, none of the receptor antibodies significantly inhibited the cytotoxic response to TNF in U-937 cells. Soluble forms of both receptors, however, completely abrogated the cellular response to TNF. Thus, these results indicate that the antibodies against both receptors together inhibit the majority of the receptor-ligand interaction without any significant effect on the biol. response to TNF.

Journal of Biological Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Stauber, Gregory B. published the artcileCharacterization and affinity cross-linking of receptors for human recombinant lymphotoxin (tumor necrosis factor-β) on a human histiocytic lymphoma cell line, U-937, COA of Formula: C18H20N2O12, the publication is Journal of Biological Chemistry (1989), 264(6), 3573-6, database is CAplus and MEDLINE.

Recombinant human lymphotoxin (rhLT) was purified and used to examine its receptors on the human histiocytic lymphoma cell line U-937. The rhLT was radioiodinated by the IODO GEN method to a specific activity of 60 μCi/μg. The specific binding reached a plateau within 10, 60, and 180 min at 37, 23, and 4°, resp. Scatchard anal. revealed the presence of a single class of high affinity receptors with an apparent K_d of 0.6 nm and a capacity of 33,000 binding sites/cell. The binding of ¹²⁵I-labeled rhLT to U-937 cells was inhibited by excess unlabeled rhLT or recombinant human tumor necrosis factor-α(rhTNF), suggesting a common receptor for both mols. The crosslinking of the receptor to rhLT revealed 2 distinct bands at approx. mol. masses of 100,000 and 120,000 daltons. Affinity crosslinking of U-937 cells with ¹²⁵I-labeled rhTNF, however, provided only a single band with a mol. mass of about 100,000 daltons. Thus, the manner in which rhLT interacts with its receptor is perhaps somewhat different from that of rhTNF.

Journal of Biological Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C16H25BO2, COA of Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts