Tag: M.W=450-500

Zhang, Meijie published the artcileEffect of hemabate combined with oxytocin on bleeding after cesarean section, Recommanded Product: 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, the publication is Xin Zhongyi (2015), 47(5), 185-186, database is CAplus.

Objective: To observe the effect of hemabate combined with oxytocin on bleeding after cesarean section. Methods: 116 cases of cesarean section women were randomly divided into a control group and an observation group. The control group received hemabate, while the observation group addnl. received oxytocin. Results: The total effective rate in the observation group was significantly higher than that in the control group. After treatment, the postpartum hemorrhage volume significantly decreased in both groups, and the observation group was significantly lower than that in the control group. There were no serious adverse reactions in both groups. Conclusion: Hemabate combined with oxytocin for maternal postoperative cesarean section can significantly reduce postpartum hemorrhage and improve clin. effect.

Xin Zhongyi published new progress about 58551-69-2. 58551-69-2 belongs to alcohols-buliding-blocks, auxiliary class Chiral,Alkenyl,Amine,Aliphatic cyclic hydrocarbon,Ester,Alcohol,Inhibitor,, name is 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, and the molecular formula is C44H28ClFeN4, Recommanded Product: 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Chen, Xiaoyu published the artcileA high throughput dimer screening assay for monoclonal antibodies using chemical cross-linking and microchip electrophoresis, Computed Properties of 70539-42-3, the publication is Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (2009), 877(27), 3012-3018, database is CAplus and MEDLINE.

A high throughput screening assay was developed to determine the total dimer level in antibody samples. This method utilizes high speed microchip electrophoresis separation following chem. crosslinking. Upon reacting with homobifunctional N-hydroxysuccinimide-esters (NHS-esters), covalent linkages can be established between the primary amines of two neighboring antibody mols. The reaction conditions are optimized to achieve quant. crosslinking of only phys. associated monomers within an antibody dimer. The resulting crosslinked dimers, originating from either covalent or non-covalent antibody dimers, can then be separated from monomers by SDS electrophoresis. A com. microchip electrophoresis instrument is used for high speed separation, allowing each sample to be analyzed in about 1 min. This approach was applied to crude mammalian cell culture samples. Using a 96-well gel filtration spin column format, interfering species in the cell culture media were efficiently removed from the samples. This method is well suited to the purpose of high throughput antibody dimer quantitation during cell culture expression, including clone selection and cell culture development. The total dimer content, both covalent and non-covalent, can be determined for hundreds of crude samples in a few hours. The effects of different crosslinking conditions on the determined dimer levels, as well as of different antibody pI values, are discussed.

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Computed Properties of 70539-42-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Murphy, Ann published the artcileChemically stabilized trypsin used in dipeptide synthesis, Application In Synthesis of 70539-42-3, the publication is Biotechnology and Bioengineering (1998), 58(4), 364-373, database is CAplus and MEDLINE.

Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester), such that approx. 8 out of 14 lysines per trypsin mol. were modified. This derivative (EG trypsin) was more stable than native between 30° and 70°: T₅₀ values were 59° and 46°, resp. EG trypsin’s half-life of 25 min at 55° was fivefold greater than native’s. EG trypsin had a decreased rate of autolysis and retained more activity in aqueous mixtures of 1,4-dioxan, DMF, dimethylsulfoxide, and acetonitrile. EG trypsin had lower K_m values for both amide and ester substrates; its k_cat values for two amides (PhCO-Arg-NHC₆H₄NO₂-4 and Cbz-Gly-Gly-Arg-NMC; NMC = 7-amino-4-methylcoumarin) increased, whereas its k_cat value for an ester (Cbz-Lys-SCOPh) decreased slightly. The specific activity (k_cat/K_m) of EG trypsin was increased for both amide and ester substrates. EG trypsin gave higher yields and reaction rates than the native trypsin in kinetically controlled synthesis of PhCO-Arg-Leu-NH₂ in acetonitrile and in t-butanol. Highest peptide yields occurred with EG trypsin in 95% acetonitrile, where 90% of the substrate was converted to product. No peptide synthesis occurred in 95% DMF with either form of trypsin.

Biotechnology and Bioengineering published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Application In Synthesis of 70539-42-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

O’Brien, Anne Marie published the artcileChemical stabilization of recombinant horseradish peroxidase, Product Details of C18H20N2O12, the publication is Biotechnology Techniques (1996), 10(12), 905-910, database is CAplus.

Unglycosylated recombinant horseradish peroxidase (HRP C°) had a half life of 21 min at 65° compared with only 5 min for the plant enzyme (HRP). The half life of HRP C^* at 65° increased by 5-fold following modification with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Tolerance to 60% 1,4-dioxan also increased while tolerance to 30% DMF was unchanged.

Biotechnology Techniques published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Product Details of C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gestin, J. F. published the artcileIntroduction of five potentially metabolizable linking groups between ¹¹¹In-cyclohexyl EDTA derivatives and F(ab’)₂ fragments of anti-carcinoembryonic antigen antibody–I. A new reproducible synthetic method, Category: alcohols-buliding-blocks, the publication is Nuclear Medicine and Biology (1993), 20(6), 755-62, database is CAplus and MEDLINE.

The purpose of this study was to synthesize new bifunctional linker-chelating agents for the modification of the in vivo distribution of ¹¹¹In-labeled antibodies. A general simple synthetic method of preparing cyclohexyl EDTA (CDTA) derivatives containing a linker/spacer group is described. Linkers prepared included a diester, a six carbon aliphatic chain, two thioethers and a disulfide group. The CDTA-linker compounds were coupled to F(Ab’)₂ fragments of anti-carcinoembryonic antigen monoclonal antibody and labeled with ¹¹¹In with good retention of immunoreactivity.

Nuclear Medicine and Biology published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Ding, Yue-He published the artcileIncreasing the Depth of Mass-Spectrometry-Based Structural Analysis of Protein Complexes through the Use of Multiple Cross-Linkers, Related Products of alcohols-buliding-blocks, the publication is Analytical Chemistry (Washington, DC, United States) (2016), 88(8), 4461-4469, database is CAplus and MEDLINE.

Chem. crosslinking of proteins coupled with mass spectrometry (CXMS) is a powerful tool to study protein folding and to map the interfaces between interacting proteins. The most commonly used cross-linkers in CXMS are BS³ and DSS, which have similar structures and generate the same linkages between pairs of lysine residues in spatial proximity. However, there are cases where no cross-linkable lysine pairs are present at certain regions of a protein or at the interface of two interacting proteins. In order to find the cross-linkers that can best complement the performance of BS³ and DSS, we tested seven addnl. cross-linkers that either have different spacer arm structures or that target different amino acids (BS²G, EGS, AMAS, GMBS, Sulfo-GMBS, EDC, and TFCS). Using BSA, aldolase, the yeast H/ACA protein complex, and E. coli 70S ribosomes, we showed that, in terms of providing structural information not obtained through the use of BS³ and DSS, EGS and Sulfo-GMBS worked better than the other cross-linkers that we tested. EGS generated a large number of cross-links not seen with the other amine-specific cross-linkers, possibly due to its hydrophilic spacer arm. We demonstrate that incorporating the cross-links contributed by the EGS and amine-sulfhydryl cross-linkers greatly increased the accuracy of Rosetta in docking the structure of the yeast H/ACA protein complex. Given the improved depth of useful information it can provide, we suggest that the multilinker CXMS approach should be used routinely when the amount of a sample permits.

Analytical Chemistry (Washington, DC, United States) published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Font, Bernard published the artcileInteraction of creatine kinase and hexokinase with the mitochondrial membranes, and self-association of creatine kinase: crosslinking studies, Related Products of alcohols-buliding-blocks, the publication is Molecular and Cellular Biochemistry (1987), 78(2), 131-40, database is CAplus and MEDLINE.

Covalent coupling of protein by crosslinking reagents has been used to study the interaction of mitochondrial creatine kinase (CKm) and hexokinase (HK) with the mitochondrial membranes. The effects of crosslinkers were studied either by following the inhibition of solubilization of enzymic activities or by modification of the electrophoretic patterns of proteins solubilized from mitochondria after treatment with different crosslinkers. Dimethylsuberimidate (DMS) efficiently reduced the amount of HK activity solubilized by various agents but it did not modify solubilization of CKm from mitochondria. The effect of DMS on HK solubilization did not result from nonspecific crosslinking since it did not impede the solubilization of adenylate kinase. The bissuccinimidyl class of crosslinkers was also tested. Ethyleneglycolbis(succinimidylsuccinate) (EGS) efficiently reduced HK solubilization, but it addnl. induced osmotic stabilization of mitochondria and thus impeded release of soluble or solubilized proteins from the intermembrane space. Furthermore, this agent drastically inhibited CKm activity and thus, in a second set of experiments the effect of crosslinkers were studied by the disappearance of protein bands in the electrophoretic pattern of soluble fractions obtained from mitochondria, the outer membranes of which have been ruptured to allow free release of soluble proteins. Results of these experiments showed that succinimidyl reagents and Cu²⁺-phenanthroline substantially reduced the amount of CKm released from mitochondria and confirmed that bisimidates were ineffective in inhibiting CKm solubilization. In addition, crosslinking reagents were used to study subunit interactions in purified CKm. The results showed, in contrast with control experiments with a monomeric protein (ovalbumin) which did not give rise to polymers, that under the same conditions, electrophoresis of crosslinked CKm resolved a set of species with mol. weights roughly equal to integral multiples of the protomer. These results prove that the polymeric form of CKm is an octamer.

Molecular and Cellular Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Freichel, Tanja published the artcileToward orthogonal preparation of sequence-defined monodisperse hetero-multivalent glyco-macromolecules on solid support using Staudinger ligation and copper-catalyzed click reactions, Category: alcohols-buliding-blocks, the publication is Journal of Organic Chemistry (2017), 82(18), 9400-9409, database is CAplus and MEDLINE.

The investigation of hetero-multivalent interactions of complex glyco-ligands and proteins is critical for understanding important biol. processes and developing carbohydrate-based pharmaceutics. Synthetic glycomimetics, derived by mimicking complex glyco-ligands on a variety of scaffolds, have become important tools for studying the role of carbohydrates in chem. and biol. In this paper, we report on a new synthetic strategy for the preparation of mono-disperse, sequence-defined glyco-oligomers or so-called precision glyco-macromols. based on solid phase oligomer synthesis and the Staudinger ligation. This strategy employs a solid-supported synthetic approach using a novel carboxy-functionalized building block which bears a functional handle required for Staudinger ligation on solid support. Furthermore, we combined Staudinger ligation and copper catalyzed azide alkyne cycloaddition (CuAAC) reactions to synthesize hetero-multivalent glyco-oligomers on solid support for the first time, demonstrating the utility of this approach for the synthesis of hetero-functional glyco-macromols.

Journal of Organic Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Zhu, Xiaofeng published the artcileChemical crosslinking approach to reveal spatial arrangement of subunits in PS II oxygen-evolving core complexes, Formula: C18H20N2O12, the publication is Zhiwu Shenglixue Tongxun (2002), 38(6), 599-602, database is CAplus.

The spatial arrangement of subunits of photosystem II O₂-evolving core complexes was analyzed by crosslinking the core complexes with bifunctional crosslinking agent [such as 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, di-Me hexanedioate, N,N- dicyclohexylcarbodiimide, etc.] at room temperature for 10 min, terminating with 10% glycine solution, centrifuging, suspending in SMN buffer (composed of sucrose 0.4, NaCl 10, and Mes-NaOH 50 mM, its pH 6.0), and detecting via SDS-PAGE.

Zhiwu Shenglixue Tongxun published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C14H26O2, Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Li, Jie published the artcileOptimum target plasma concentration of propofol in preventing adverse effects of carboprost tromethamine in patients undergoing caesarean section, Safety of 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, the publication is Zhonghua Mazuixue Zazhi (2016), 36(3), 321-324, database is CAplus.

Objective To investigate the optimum target plasma concentration of propofol in preventing the adverse effects of carboprost tromethamine in the patients undergoing caesarean section. Methods One hundred and twenty-eight nulliparous parturients who were at full term with a singleton fetus, of American Society of Anesthesiologists phys. status I or II, aged 24-37 yr, weighing 54-78 kg, scheduled for elective caesarean section under combined spinal-epidural anesthesia, were randomly divided into 4 groups (n = 32 each) using a random number table: control group (group C) and different concentrations of propofol groups (P_1-3 groups). Carboprost tromethamine 250 μg was injected into the body of the uterus, and propofol with the target plasma concentrations of 0.8, 1.2 and 1.6 μg/mL was simultaneously given by target-controlled infusion in P₁, P₂ and P₃ groups, resp., and normal saline was infused at a rate of 0.5 mL·kg^-1·h^-1 in group C. The occurrence of cardiovascular events was recorded from the end of carboprost tromethamine administration until the end of surgery. The related adverse effects after carboprost tromethamine administration and Ramsay sedation score at 15 mm after carboprost tromethamine administration were recorded, and satisfactory sedation was defined as Ramsay sedation score of 2. The occurrence of complications associated with combined spinal-epidural anesthesia was recorded during the postoperative follow-up. Results Compared with group C, the incidence of carboprost tromethamine-related adverse effects was significantly decreased in P₂ and P₃ groups, the rate of satisfactory sedation was significantly increased in P₁ and P₂ groups, the incidence of hypotension and tachycardia was significantly decreased in group P₁ (P<0.05), and no significant change was found in the incidence of carboprost tromethamine-related adverse effects in group P₁ and in the rate of satisfactory sedation in group P₃ (P>0.05). Compared with group P₁, the incidence of carboprost tromethamine-related adverse effects was significantly decreased in P₂ and P₃ groups, the rate of satisfactory sedation was significantly increased in group P₂, and the rate of satisfactory sedation was significantly decreased in group P₃ (P<0.05). Compared with group P₂, the rate of satisfactory sedation was significantly decreased (P<0.05), and no significant change was found in the incidence of carboprost tromethamine-related adverse effects in group P₃ (P>0.05). No cardiovascular events were found in group P₂, and the incidence of hypotension was 9% in group P₃. Conclusion The optimum target plasma concentration of propofol in preventing the adverse effects of carboprost tromethamine is 1.2 μg/mL in the patients undergoing caesarean section.

Zhonghua Mazuixue Zazhi published new progress about 58551-69-2. 58551-69-2 belongs to alcohols-buliding-blocks, auxiliary class Chiral,Alkenyl,Amine,Aliphatic cyclic hydrocarbon,Ester,Alcohol,Inhibitor,, name is 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, and the molecular formula is C25H47NO8, Safety of 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts