Tag: M.W=450-500

Anagnostou, Athanasius published the artcileErythropoietin has a mitogenic and positive chemotactic effect on endothelial cells, Product Details of C18H20N2O12, the publication is Proceedings of the National Academy of Sciences of the United States of America (1990), 87(15), 5978-82, database is CAplus and MEDLINE.

A dose-dependent proliferative action of human recombinant erythropoietin on human umbilical vein endothelial cells and bovine adrenal capillary endothelial cells was observed Binding studies with radioiodinated recombinant human erythropoietin revealed a large number (≈27,000) of an apparent single class of receptors with an affinity in the 10^-9 M range. Linkage of the radiolabeled ligand to its receptor via a bifunctional crosslinking agent allowed identification of an endothelial cell protein of 45 kilodaltons as the principal receptor associated with this mitogenic effect of erythropoietin. Recombinant human erythropoietin also enhanced the migration of endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Product Details of C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Iwuoha, Emmanuel I. published the artcileReactivities of Organic-Phase Biosensors. 1. Enhancement of the Sensitivity and Stability of Amperometric Peroxidase Biosensors Using Chemically Modified Enzymes, Application of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Analytical Chemistry (1997), 69(8), 1674-1681, database is CAplus.

Amperometric organic- and aqueous-phase peroxide biosensors were prepared with native and N-hydroxysuccinimide ester (NHS)-modified horseradish peroxidase (HRP). The ε-amino functions of the six free lysine residues of HRP were selectively modified with homobifunctional suberic acid bis(N-hydroxysuccinimide ester) (SA-NHS) or ethylene glycol bis(succinic acid N-hydroxysuccinimide ester) (EG-NHS). The biosensors were based on electrostatic complexation of the HRP variants with an osmium bis(bipyridyl)poly(4-vinylpyridine) polymer. The enzyme electrodes were operated in both aqueous (0.1 M phosphate buffer, pH 7.0) and organic (90% CH₃CN) phases, for the detection of hydrogen peroxide and Me isothiocyanate. Kinetic anal. of the steady-state amperometry data showed that chem. modification of HRP about up to a ∼5-fold enhancement of the biosensor sensitivity for H₂O₂ and an improvement in both sensor stability and organotolerance. Also, NHS modification of HRP results in a 3-fold extension of the range of linear response of the peroxide sensor in both aqueous and nonaqueous media. When used as a Me isothiocyanate sensor in the organic phase, the amperometric responses of the biosensors were doubled on changing from native HRP to NHS-HRP electrodes.

Analytical Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Application of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Anagnostou, Athanasius published the artcileErythropoietin has a mitogenic and positive chemotactic effect on endothelial cells, Product Details of C18H20N2O12, the publication is Proceedings of the National Academy of Sciences of the United States of America (1990), 87(15), 5978-82, database is CAplus and MEDLINE.

A dose-dependent proliferative action of human recombinant erythropoietin on human umbilical vein endothelial cells and bovine adrenal capillary endothelial cells was observed Binding studies with radioiodinated recombinant human erythropoietin revealed a large number (≈27,000) of an apparent single class of receptors with an affinity in the 10^-9 M range. Linkage of the radiolabeled ligand to its receptor via a bifunctional crosslinking agent allowed identification of an endothelial cell protein of 45 kilodaltons as the principal receptor associated with this mitogenic effect of erythropoietin. Recombinant human erythropoietin also enhanced the migration of endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Product Details of C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Vijayasree, P. published the artcileA simple RP-HPLC method for quantitation of carboprost tromethamine in injection dosage form, Recommanded Product: 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, the publication is Journal of Global Trends in Pharmaceutical Sciences (2014), 5(4), 2012-2016, database is CAplus.

A new simple, precise, accurate and rapid method was developed for determination of Carboprost Tromethamine from its pharmaceutical dosage form. The separation was carried out on a Waters C18 column (Symmetry -C18, 3.5μm, and 4.6×100mm) in isocratic mode with mobile phase comprising acetate buffer pH 3.7 and methanol (30:70, %volume/volume) by using Agilent 1100 series HPLC system with Agilent Chemstation software. The flow rate was 1 mL/min and Ultra-Violet detection was carried out at 200 nm. Every part of determination was performed at ambient column temperature The retention time was 6.827 min for Carboprost Tromethamine. The developed method was validated for parameters like specificity, accuracy, precision, robustness as per International Conference on Harmonization guidelines. Linearity for Carboprost Tromethamine was in the range of 83-249μg/mL and Correlation coefficient was found to be 0.999. The percentage recovery was found to be in the limit of 98.4-99.3 %. Statistical anal. of the results has been carried out revealing high accuracy and good precision. Hence this method can be of use and value for the quality control department of pharmaceutical companies manufacturing these formulations without any interference due its sensitivity, simplicity and selectivity.

Journal of Global Trends in Pharmaceutical Sciences published new progress about 58551-69-2. 58551-69-2 belongs to alcohols-buliding-blocks, auxiliary class Chiral,Alkenyl,Amine,Aliphatic cyclic hydrocarbon,Ester,Alcohol,Inhibitor,, name is 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, and the molecular formula is C8H5F3N4, Recommanded Product: 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Anantram, Aarti published the artcileMolecular dynamic simulations on an inhibitor of anti-apoptotic Bcl-2 proteins for insights into its interaction mechanism for anti-cancer activity, Quality Control of 518303-20-3, the publication is Journal of Biomolecular Structure and Dynamics (2019), 37(12), 3109-3121, database is CAplus and MEDLINE.

The first anticancer agents targeting this family of proteins were aimed primarily toward inhibition of Bcl-2. An elevated level of Mcl-1, despite Bcl-2 inhibition, continues to be a cause for resistance in most cancers. However, in silico exploration of Mcl-1 specific drugs and their associated mechanisms have not been clearly elucidated. In order to understand the same, we have carried out docking and mol. dynamic simulations on ABT-263 (Navitoclax), an orally active inhibitor of Bcl-2, Bcl-xL, and Bcl-w proteins; Obatoclax, a pan-Bcl-2 inhibitor as well as Maritoclax, an Mcl-1 specific inhibitor. Docking studies revealed that binding to the hydrophobic grooves is a prerequisite for action on the BCL protein and the binding mechanism and chem. space utilization dictates stability as well as specificity of the inhibitor mol. dynamic simulations showed that on binding, the a-helixes of these proteins exhibited less fluctuations than loop regions, also hydrophobic contacts and hydrogen bonding were observed to be the predominant interactions in the drug-receptor complexes.Communicated by Ramaswamy H. Sarma.

Journal of Biomolecular Structure and Dynamics published new progress about 518303-20-3. 518303-20-3 belongs to alcohols-buliding-blocks, auxiliary class Apoptosis,Bcl-2, name is 2-((4-(4-Bromophenylsulfonamido)-1-hydroxynaphthalen-2-yl)thio)acetic acid, and the molecular formula is C18H14BrNO5S2, Quality Control of 518303-20-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Boivin, Patrick published the artcileImmobilization of perfluoroalkylated enzymes in a biologically active state onto Perflex support, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biotechnology and Applied Biochemistry (1991), 14(2), 155-69, database is CAplus and MEDLINE.

Perflex was introduced as a new fluorocarbon-based technol. for protein immobilization. Due to the hydrophobic character of the support, however, significant loss of enzymic activity may occur upon immobilization of certain enzymes, which appears to be due to a large conformational change of the protein (inversion). Pretreatment of the Perflex support with a neutral fluorosurfactant lessened the surface hydrophobicity, thus decreasing the hydrophobic interaction between the support and the protein. Modification of enzymes with a high number of fluorocarbon residues, which forms a hydrophobic envelope around the protein, also appeared to prevent enzyme inactivation upon immobilization on Perflex support. Moreover, preactivation of the support with either perfluorooctylpropylisocyanate or reactive poly(fluoroalkyl)sugar reagents greatly improved the enzyme particle activity by increasing the amount of immobilized enzyme. Fluorosurfactant treatment of the support activated with perfluorooctylpropylisocyanate improved the retention of activity for sensitive enzymes such as chymotrypsin and increased the wet ability and ease of handling of the Perflex particles.

Biotechnology and Applied Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Isaacs, W. B. published the artcileAssociation of titin and myosin heavy chain in developing skeletal muscle, Category: alcohols-buliding-blocks, the publication is Proceedings of the National Academy of Sciences of the United States of America (1992), 89(16), 7496-500, database is CAplus and MEDLINE.

To understand mol. interactions that organize developing myofibrils, the biosynthesis and interaction of titin and myosin heavy chain in cultures of developing chicken leg myoblasts were examined Use of pulse-labeling, immunoprecipitation, and a reversible crosslinking procedure demonstrates that within minutes of synthesis, titin and myosin heavy chain can be chem. crosslinked into very large, detergent-resistant complexes retaining many features of intact myotubes. These complexes, predominantly of titin and myosin, occur very early in myofibrillogenesis as well as later. Apparently, synthesis and assembly of titin and myosin are temporally and spatially coordinated in nascent myofibrils, and titin mols. may help to organize sarcomere formation.

Proceedings of the National Academy of Sciences of the United States of America published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Min, Kyueng-Whan published the artcileHigh polymerase ε expression associated with increased CD8+T cells improves survival in patients with non-small cell lung cancer, Category: alcohols-buliding-blocks, the publication is PLoS One (2020), 15(5), e0233066, database is CAplus and MEDLINE.

DNA replicase polymerase ε (POLE) is critical in proofreading and correcting errors of DNA replication. Low POLE expression plays a pivotal role in accumulation of mutations and onset of cancer, contributing to development and growth of tumor cells. The aim of this study is to reveal the survival, alternative genes and antitumoral immune activities in non-small cell lung cancer (NSCLC) patients with low POLE expression and provide treatment strategies that can increase their survival rates. This study investigated the clinicopathol. parameters, various tumor-infiltrating lymphocytes (TILs), endogenous retrovirus, mol. interactions and in vitro drug screen according to POLE mutation/expression in 168 and 1,019 NSCLC patients from the Konkuk University Medical Center (KUMC) and the Cancer Genome Atlas, resp. We identified mutations of 75 genes in the sequencing panels, with POLE frame shift p.V1446fs being the most frequent (56.8%) in KUMC based on 170 targeted sequencing panels. Mutant and high expression of POLE correlated with favorable prognosis with increased TILs and tumor mutation burden, compared with wild type and low expression of POLE. We found specific mol. interactions associated with cell cycle and antigen presentation. An in vitro drug screen identified dasatinib that inhibited growth of the NSCLC cell line with low POLE expression. POLE could contribute to the future development of anticancer drugs for patients with NSCLC.

PLoS One published new progress about 518303-20-3. 518303-20-3 belongs to alcohols-buliding-blocks, auxiliary class Apoptosis,Bcl-2, name is 2-((4-(4-Bromophenylsulfonamido)-1-hydroxynaphthalen-2-yl)thio)acetic acid, and the molecular formula is C18H14BrNO5S2, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Habibi, Azadeh Ebrahim published the artcileThermostabilization of Bacillus amyloliquefaciens α-amylase by chemical crosslinking, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Journal of Biotechnology (2006), 123(4), 434-442, database is CAplus and MEDLINE.

Chem. crosslinking of a mesophilic α-amylase from Bacillus amyloliquefaciens (BAA) was carried out. Intramol. crosslinks between Lys residues upon treatment of the enzyme with ethylene glycol bis(succinic acid N-hydroxy succinimide ester) resulted in enhancement of thermostability as indicated by irreversible thermoinactivation experiments Enhancement of thermostability coincided with a dramatic protection against aggregation, combined with a decrease in surface hydrophobicity. Deamidation, another important mechanism of irreversible thermoinactivation, was also diminished upon modification. While no significant changes in the kinetic parameters were evident, rigidification of the protein structure was suggested by CD and fluorescence studies.

Journal of Biotechnology published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Zhou, Jie published the artcileSimultaneous determination of the main components in carboprost tromethamine injection by HPLC-UV-CAD, Quality Control of 58551-69-2, the publication is Zhongguo Xinyao Zazhi (2015), 24(1), 22-26, 51, database is CAplus.

Objective: To establish an HPLC-UV-CAD method for the simultaneous determination of carboprost, tromethamine and benzyl alc. in carboprost tromethamine injection. Methods: Carboprost, tromethamine and benzyl alc. were separated on a CAPCELL PAK CR 1:4 column (150 mm×4.6 mm, 5 μm). The mobile phase was composed of ultrapure water, 0.1 mol·L^-1 ammonium acetate buffer (pH 6.2) and methanol with gradient elution mode at a flow about 1.0 mL·min^-1; the injection volume was 10 μL and column temperature was 40 °C. CAD was used to detect carboprost and tromethamine, in which nebulization temperature was 35 °C and collection frequency was 30 Hz. UV was used to detect benzyl alc., in which detection wavelength was set at 258 nm and collection frequency was 5 Hz. Results: The linear range of carboprost was 9.38-56.2 μg·mL^-1 (r = 0.997 1, n = 6); the lowest limit of detection was 0.3 μg·mL^-1; LOQ was 0.6 μg·mL^-1; average recovery was 104.7%. The linear range of tromethamine was 5.19-23.3 μg·mL^-1 (r = 0.999 2, n = 6); the lowest limit of detection was 1 μg·mL^-1; LOQ was 2.5 μg·mL^-1; average recovery was 95.5%. The linear range of benzyl alc. was 0.297-1.78 mg·mL^-1 (r = 1, n = 6), the lowest limit of detection was 5.0 μg·mL^-1; LOQ was 10.0 μg·mL^-1; average recovery was 100.1%. The actual measurement results of the sample were consistent with the standard method currently used. Conclusion: The HPLC method established does not require derivative operations, and is cheap, sensitive, simple, accurate, reproducible and accurate. This method can simultaneously detect carboprost, tromethamine and benzyl alc. in carboprost tromethamine injection.

Zhongguo Xinyao Zazhi published new progress about 58551-69-2. 58551-69-2 belongs to alcohols-buliding-blocks, auxiliary class Chiral,Alkenyl,Amine,Aliphatic cyclic hydrocarbon,Ester,Alcohol,Inhibitor,, name is 2-Amino-2-(hydroxymethyl)propane-1,3-diol (Z)-7-((1R,2R,3R,5S)-3,5-dihydroxy-2-((S,E)-3-hydroxy-3-methyloct-1-en-1-yl)cyclopentyl)hept-5-enoic acid, and the molecular formula is C5H6BNO2, Quality Control of 58551-69-2.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts