Tag: 600-700

DNA-Mediated Liposome Fusion Observed by Fluorescence Spectrometry was written by Loffler, Philipp M. G.;Ries, Oliver;Vogel, Stefan. And the article was included in Methods in Molecular Biology in 2020.Reference of 923-61-5 The following contents are mentioned in the article:

DNA-programmed and controlled fusion of lipid membranes have recently been optimized to reliably mix the contents between two populations of liposomes, each functionalized with complementary lipidated DNA (LiNA) oligomer. In this chapter we describe a procedure for DNA-controlled fusion of liposomes mediated by LiNAs that are designed to force bilayers into close proximity. Using a self-quenching fluorescent dye (Sulforhodamine B) to monitor both the mixing of the internal volumes and leakage of the dye into the outer volume we measure the efficiency of content mixing in the bulk population, allowing for direct comparison between different LiNA designs. By generating samples for calibration corresponding to different amounts of content mixing, the average number of fusion events per labeled liposome can be estimated This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Reference of 923-61-5).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.Reference of 923-61-5

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Capacitance extraction method for a free-standing bilayer lipid membrane formed over an aperture in a nanofabricated silicon chip was written by Tomioka, Yasutaka;Takashima, Shogo;Moriya, Masataka;Shimada, Hiroshi;Hirose, Fumihiko;Hirano-Iwata, Ayumi;Mizugaki, Yoshinao. And the article was included in Japanese Journal of Applied Physics in 2020.COA of Formula: C37H74NO8P The following contents are mentioned in the article:

A bilayer lipid membrane (BLM) is the main component of the cell membrane of living organisms, which can be formed artificially. Although the specific capacitance of a BLM is known to be in the range of 0.4-1.0μF cm-2, many previous works that formed free-standing BLMs over an aperture in silicon chips reported larger values beyond this typical range, which suggests that equivalent-circuit models are not adequate. In this work, we modified the equivalent-circuit model by adding a resistance element of silicon. To evaluate the validity of the modified model, we applied the model to the results of electrochem. impedance spectroscopy for free-standing BLMs formed over an aperture in nanofabricated silicon chips. The derived specific capacitance values were 0.57 ± 0.08μF cm-2, which settles in the typical range. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5COA of Formula: C37H74NO8P).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.COA of Formula: C37H74NO8P

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane was written by Gabba, Matteo;Frallicciardi, Jacopo;van ‘t Klooster, Joury;Henderson, Ryan;Syga, Lukasz;Mans, Robert;van Maris, Antonius J. A.;Poolman, Bert. And the article was included in Biophysical Journal in 2020.Formula: C37H74NO8P The following contents are mentioned in the article:

The authors present a fluorescence-based approach for determination of the permeability of small mols. across the membranes of lipid vesicles and living cells. With properly designed experiments, the method allows the authors to assess the membrane phys. properties both in vitro and in vivo. The permeability of weak acids increases in the order of benzoic > acetic > formic > lactic, both in synthetic lipid vesicles and the plasma membrane of Saccharomyces cerevisiae, but the permeability is much lower in yeast (one to two orders of magnitude). A relation between the mol. permeability and the saturation of the lipid acyl chain (i.e., lipid packing) in the synthetic lipid vesicles. were observed By analyzing wild-type yeast and a manifold knockout strain lacking all putative lactic acid transporters, the yeast plasma membrane is impermeable to lactic acid on timescales up to ∼2.5 h. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Formula: C37H74NO8P).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Formula: C37H74NO8P

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Budding and Division of Giant Vesicles Linked to Phospholipid Production was written by Castro, Juan M.;Sugiyama, Hironori;Toyota, Taro. And the article was included in Scientific Reports in 2019.Reference of 923-61-5 The following contents are mentioned in the article:

The self-reproduction of supramol. assemblies based on the synthesis and self-assembly of building blocks is a critical step towards the construction of chem. systems with autonomous, adaptive, and propagation properties. In this report, we demonstrate that giant vesicles can grow and produce daughter vesicles by synthesizing and incorporating phospholipids in situ from ad-hoc precursors. Our model involves acyl chain elongation via copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition reaction and the ensuing production of synthetic phospholipids to induce budding and division. In addition, the growth and budding of giant vesicles were compatible with the encapsulation and transfer of macromols. as large as lambda phage DNA to the buds. This chem. system provides a useful model towards the implementation of cell-like compartments capable of propagation and transport of biol. materials. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Reference of 923-61-5).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Reference of 923-61-5

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Biological activity of pectic polysaccharides investigated through biomembrane models formed at the air-water interface was written by de Andrade Escobar, Bruno;Valerio, Gabriel Lundgren Ferreira;Caseli, Luciano. And the article was included in Colloids and Surfaces, B: Biointerfaces in 2022.Quality Control of (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate The following contents are mentioned in the article:

Pectin, a polysaccharide with potential bioactivity, was inserted in the aqueous subphase of monolayers of the selected lipids DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and DPPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine), representing mammalian and bacterial membranes, resp. Pectin condensed both monolayers but made the DPPC monolayer more fluid, while for DPPE, it made its monolayer more rigid, as detected with dynamic interfacial rheol. Complementary data using surface potential, IR spectroscopy, and Brewster angle microscopy also showed distinctive effects of pectin on DPPE and DPPC. We believe these data can be correlated with the action of this polysaccharide with biol. lipidic surfaces with different polar heads, which may be relevant, generally speaking, to understanding the mol. mechanism of this bioactive compound for pharmaceutical purposes. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Quality Control of (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Quality Control of (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin was written by Kim, Eunsol;Kim, Jong Chul;Min, Kiyoon;Goh, MeeiChyn;Tae, Giyoong. And the article was included in ACS Applied Materials & Interfaces in 2018.Name: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate The following contents are mentioned in the article:

Cell aggregates hold significant therapeutic promise for in vitro cell anal., ex vivo tissue models, and in vivo cell therapy and tissue engineering. Traditional methods of making cell aggregates require long incubation times and can only produce three-dimensional-spheroid-shaped aggregates. We propose a novel method of making cell aggregates of diverse sizes and shapes using lipid-conjugated heparin. Shaking the cell suspension containing a small amount of lipid-conjugated heparin for approx. 30 min produced cell aggregates. This approach can be applied to any cell type, including stem cells, fibroblast cells, and T lymphocytes. The shape of biocompatible templates could modulate the shape of cell aggregates. In addition to layered, multicompartmental cell aggregates on template, template-free, tube-shaped cell aggregates could also be made. The cell aggregates formed were alive and maintained biol. activities. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Name: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Alkyl halides are often synthesized from alcohols, in effect substituting a halogen atom for the hydroxyl group. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.Name: (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Machine Learning Algorithm Identifies an Antibiotic Vocabulary for Permeating Gram-Negative Bacteria was written by Mansbach, Rachael A.;Leus, Inga V.;Mehla, Jitender;Lopez, Cesar A.;Walker, John K.;Rybenkov, Valentin V.;Hengartner, Nicolas W.;Zgurskaya, Helen I.;Gnanakaran, S.. And the article was included in Journal of Chemical Information and Modeling in 2020.COA of Formula: C37H74NO8P The following contents are mentioned in the article:

Drug discovery faces a crisis. The industry has used up the “obvious” space in which to find novel drugs for biomedical applications, and productivity is declining. One strategy to combat this is rational approaches to expand the search space without relying on chem. intuition, to avoid rediscovery of similar spaces. In this work, we present proof of concept of an approach to rationally identify a “chem. vocabulary” related to a specific drug activity of interest without employing known rules. We focus on the pressing concern of multidrug resistance in Pseudomonas aeruginosa by searching for submols. that promote compound entry into this bacterium. By synergizing theory, computation, and experiment, we validate our approach, explain the mol. mechanism behind identified fragments promoting compound entry, and select candidate compounds from an external library that display good permeation ability. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5COA of Formula: C37H74NO8P).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.COA of Formula: C37H74NO8P

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Coupled Membrane Lipid Miscibility and Phosphotyrosine-Driven Protein Condensation Phase Transitions was written by Chung, Jean K.;Huang, William Y. C.;Carbone, Catherine B.;Nocka, Laura M.;Parikh, Atul N.;Vale, Ronald D.;Groves, Jay T.. And the article was included in Biophysical Journal in 2021.Synthetic Route of C37H74NO8P The following contents are mentioned in the article:

Lipid miscibility phase separation has long been considered to be a central element of cell membrane organization. More recently, protein condensation phase transitions, into three-dimensional droplets or in two-dimensional lattices on membrane surfaces, have emerged as another important organizational principle within cells. Here, we reconstitute the linker for activation of T cells (LAT):growth-factor-receptor-bound protein 2 (Grb2):son of sevenless (SOS) protein condensation on the surface of giant unilamellar vesicles capable of undergoing lipid phase separations Our results indicate that the assembly of the protein condensate on the membrane surface can drive lipid phase separation This phase transition occurs isothermally and is governed by tyrosine phosphorylation on LAT. Furthermore, we observe that the induced lipid phase separation drives localization of the SOS substrate, K-Ras, into the LAT:Grb2:SOS protein condensate. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Synthetic Route of C37H74NO8P).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. The oxygen atom of the strongly polarized O―H bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.Synthetic Route of C37H74NO8P

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Experimental Evidence of the Existence of Interleaflet Coupled Nanodomains: An MC-FRET Study was written by Vinklarek, Ivo S.;Vel’as, Lukas;Riegerova, Petra;Skala, Kristian;Mikhalyov, Ilya;Gretskaya, Natalia;Hof, Martin;Sachl, Radek. And the article was included in Journal of Physical Chemistry Letters in 2019.Electric Literature of C37H74NO8P The following contents are mentioned in the article:

Plasma membranes of living cells are compartmentalized into small submicroscopic structures (nanodomains) having potentially relevant biol. functions. Despite this, structural features of these nanodomains remain elusive, primarily due to the difficulties in characterizing such small dynamic entities. It is unclear whether nanodomains found in the upper bilayer leaflet are transversally registered with those found in the lower leaflet. Experiments performed on larger microscopic domains indicate that the coupling between the leaflets is strong, forcing the domains to be in perfect registration, but can the same thing be said about the biol. more relevant nanodomains. This work provides exptl. evidence that even small nanodomains of variable sizes between 10 and 160 nm are interleaflet coupled. Importantly, the alternative scenarios of partially registered, independent, or antiregistered nanodomains could be excluded. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Electric Literature of C37H74NO8P).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Electric Literature of C37H74NO8P

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Immobilized Lipid Affinity Capture for Antimicrobial Peptides Screening was written by Joo, Hwang-Soo. And the article was included in Biotechnology and Bioprocess Engineering in 2018.Category: alcohols-buliding-blocks The following contents are mentioned in the article:

A novel method for rapid screening of antimicrobial peptides (AMPs) was developed by using immobilized lipid affinity capture (ILAC) coupled with LC-MS. Phospholipid (PL) mixture containing phosphatidyl glycerol (PG): phosphatidyl ethanolamine (PE) (4:1), roughly mimic the PL composition of Gram-pos. bacterial membrane, was covalently immobilized on magnetic particles (MPs). PL monolayer immobilized on MPs was used as a matrix for capturing of the membrane-disruptive AMPs. Hominicin, a new AMP against Gram-pos. bacteria, was successfully captured by ILAC from the peptide pool of Staphylococcus hominis MBBL 2-9. The hominicin was identified by the comparative anal. of LC-MS 2Dprofiles of peptides captured by bare and PL-immobilized MPs. This is the first report for the development of rapid AMP screening method using lipid-immobilized MPs and LC-MS which will be a promising tool for discovery of various kinds of AMPs. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Category: alcohols-buliding-blocks).

(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Tertiary alcohols cannot be oxidized at all without breaking carbon-carbon bonds, whereas primary alcohols can be oxidized to aldehydes or further oxidized to carboxylic acids.Category: alcohols-buliding-blocks

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts