Category: 85618-21-9

Richter, David published the artcileZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement, Formula: C14H28O5S, the publication is Scientific Reports (2019), 9(1), 1-16, database is CAplus and MEDLINE.

The recently evolved field of synthetic biol. has revolutionized the way we think of biol. as an “engineerable” discipline. The newly sprouted branch is constantly in need of simple, cost-effective and automatable DNA-assembly methods. We have developed a reliable DNA-assembly system, ZeBRα (Zero-Background Redα), for cloning multiple DNA-fragments seamlessly with very high efficiency. The hallmarks of ZeBRα are the greatly reduced hands-on time and costs and yet excellent efficiency and flexibility. ZeBRα combines a “zero-background vector” with a highly efficient in vitro recombination method. The suicide-gene in the vector acts as placeholder, and is replaced by the fragments-of-interest, ensuring the exclusive survival of the successful recombinants. Thereby the background from uncut or re-ligated vector is absent and screening for recombinant colonies is unnecessary. Multiple fragments-of-interest can be assembled into the empty vector by a recombinogenic E. coli-lysate (SLiCE) with a total time requirement of less than 48 h. We have significantly simplified the preparation of the high recombination-competent E. coli-lysate compared to the original protocol. ZeBRα is the least labor intensive among comparable state-of-the-art assembly/cloning methods without a trade-off in efficiency.

Scientific Reports published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Formula: C14H28O5S.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Kowatz, Thomas published the artcileCharacterization of human β,β-carotene-15,15′-monooxygenase (BCMO1) as a soluble monomeric enzyme, Recommanded Product: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Archives of Biochemistry and Biophysics (2013), 539(2), 214-222, database is CAplus and MEDLINE.

The formal first step in vitamin A metabolism is the conversion of its natural precursor β,β-carotene (C40) to retinaldehyde (C20). This reaction is catalyzed by the enzyme β,β-carotene-15,15′-monooxygenase (BCMO1). BCMO1 has been cloned from several vertebrate species, including humans. However, knowledge about this protein’s enzymic and structural properties is scant. Here we expressed human BCMO1 in Spodoptera frugiperda 9 insect cells. Recombinant BCMO1 is a soluble protein that displayed Michaelis-Menten kinetics with a K_m of 14 μM for β,β-carotene. Though addition of detergents failed to increase BCMO1 enzymic activity, short chain aliphatic detergents such as C₈E₄ and C₈E₆ decreased enzymic activity probably by interacting with the substrate binding site. Thus we purified BCMO1 in the absence of detergent. Purified BCMO1 was a monomeric enzymically active soluble protein that did not require cofactors and displayed a turnover rate of about 8 mols. of β,β-carotene per s. The aqueous solubility of BCMO1 was confirmed in mouse liver and mammalian cells. Establishment of a protocol that yields highly active homogenous BCMO1 is an important step towards clarifying the lipophilic substrate interaction, reaction mechanism and structure of this vitamin A forming enzyme.

Archives of Biochemistry and Biophysics published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Recommanded Product: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Ogawa, Shigesaburo published the artcileGlass transition behavior of octyl β-D-glucoside and octyl β-D-thioglucoside/water binary mixtures, Category: alcohols-buliding-blocks, the publication is Carbohydrate Research (2010), 345(17), 2534-2541, database is CAplus and MEDLINE.

The lyotropic behavior and glass-forming properties of octyl β-D-glucoside (C8Glu) and octyl β-D-thioglucoside (C8SGlu)/water binary mixtures were evaluated using differential scanning calorimetry and polarizing optical microscopy. The mixture formed a glass in the supercooling state of liquid crystalline phases such as cubic, lamellar, and smectic. The glass transition temperature (T_g) of the mixture was strongly dependent on solute concentration, with a higher concentration correlating with a higher T_g. The exptl. T_g was consistent with the predicted value calculated using the Couchman-Karasz equation in both the C8Glu and C8SGlu/water mixtures The change of heat capacity at T_g showed the two bending points under variation of concentrations And the highest temperature of phase transition from lamellar to isotropic solution was observed at around 50% molar concentration It was expected that non-percolated state of water existed in extremely higher concentration ranges.

Carbohydrate Research published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Hibi, Takao published the artcilePurification of the functional plant membrane channel KAT1, Product Details of C14H28O5S, the publication is Biochemical and Biophysical Research Communications (2008), 374(3), 465-469, database is CAplus and MEDLINE.

The inward-rectifying K⁺ channel KAT1 is expressed mainly in Arabidopsis thaliana guard cells. The purification of functional KAT1 has never been reported. The authors investigated the extraction of the plant K⁺ channel KAT1 with different detergents, as an example for how to select detergents for purifying a eukaryotic membrane protein. A KAT1-GFP fusion protein was used to screen a library of 46 detergents for the effective solubilization of intact KAT1. Then, a “test set” of three detergents was picked for further anal., based on their biochem. characteristics and availability. The combination use of the selected detergents enabled the effective purification of functional KAT1 with affinity and gel-filtration chromatog.

Biochemical and Biophysical Research Communications published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Product Details of C14H28O5S.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Hierrezuelo, J. M. published the artcileMicellar properties of a mixed surfactant system constituted by n-octyl-β-D-thioglucopyranoside and sodium dodecyl sulphate, Related Products of alcohols-buliding-blocks, the publication is Colloids and Surfaces, A: Physicochemical and Engineering Aspects (2005), 264(1-3), 29-36, database is CAplus.

A fluorescence probe study on micellization of the binary surfactant system formed by n-octyl-β-D-thioglucopyranoside (OTG) and Na dodecyl sulfate (SDS) in 0.1M NaCl aqueous solutions was carried out. The CMC exptl. data, as obtained by the pyrene 1:3 ratio method, were analyzed in the context of the pseudophase separation model. The interaction parameter (β₁₂) determined by using the regular solution theory (RST) is neg. and independent on the solution composition The exptl. data on micellar composition showed a good agreement with those predicted by RST. Data reported by a treatment based on the Gibbs-Duhem equation showed a reasonable agreement with those obtained by RST, suggesting that the mixed system behaves as a regular solution From the mixing thermodn. function values can be inferred that the electrostatic repulsions between the sulfate groups of SDS control the stability of the mixed micelles. Size of micelles was examined through the micellar aggregation number as obtained by the static quenching method. The aggregation number initially increases, giving a maximum value at low content of the ionic component, remaining almost constant at larger content of this one. This behavior was interpreted from the role played by the electrostatic repulsions between the headgroups of SDS in the stabilization of the mixed micelles. Finally, studies based on both pyrene 1:3 ratio index and intramol. excimer forming of 1,3-dipyrenylpropane solubilized in the micellar phase, reported information on the microenvironmental properties of mixed micelles. The results obtained were rationalized from 2 effects: the electrostatic repulsions between the headgroups of the ionic component and the micellar hydration.

Colloids and Surfaces, A: Physicochemical and Engineering Aspects published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Molina-Bolivar, J. A. published the artcileSurface Activity, Micelle Formation, and Growth of n-Octyl-β-D-Thioglucopyranoside in Aqueous Solutions at Different Temperatures, SDS of cas: 85618-21-9, the publication is Journal of Physical Chemistry B (2004), 108(34), 12813-12820, database is CAplus.

Micellization, surface activity, and structures of the aggregates of the nonionic surfactant n-octyl-β-D-thioglucopyranoside in aqueous solutions through a temperature range have been investigated. By using surface tension measurements, information was obtained on both changes in the critical micelle concentration and adsorption behavior in the air-liquid interface with the temperature These data were used to obtain the thermodn. properties of micellization along with the corresponding adsorption parameters in the air-liquid interface. The results obtained indicated that the surfactant headgroup has a more pronounced hydrophilic character than that of the common polyoxyethylenic nonionic surfactants. Size and structure of the micelles formed at different temperatures were investigated by light scattering measurements. The light scattering data, including static and dynamic experiments, account for micelle growth and hydration. The anal. of the exptl. results was focused on the phase transition from sphere to rodlike structures. To corroborate this point, addnl. measurements of d. and intramol. excimer formation of 1,2-dypyrenylpropane vs. the surfactant concentration were performed. Satisfactory agreement of the results, showing a significant micellar growth starting from a certain surfactant concentration, was observed by means of the different techniques used.

Journal of Physical Chemistry B published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, SDS of cas: 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Naous, M. published the artcileSynergism in mixtures of n-octyl-β-D-thioglucoside and different n-alkyltrimethylammonium bromides: effect of the alkyl chain length, Synthetic Route of 85618-21-9, the publication is Colloid and Polymer Science (2012), 290(11), 1041-1051, database is CAplus.

Mixtures of n-octyl-β-D-thioglucoside with three different n-alkyltrimethylammonium bromides (n = 12 (DTAB), 14 (TTAB), and 16 (CTAB)) have been studied by using fluorescence spectroscopic techniques. The critical micelle concentration values of pure and mixed systems were determined by the pyrene 1:3 ratio method. The exptl. results were treated by using thermodn. mixing approaches based on the pseudo-phase separation model. All the mixed systems show a neg. deviation from ideal behavior, more pronounced with a larger alkyl tail of the co-surfactant. The three mixed systems fulfill the conditions of synergism, this behavior being also dependent on the alkyl chain length. By using the static quenching method, the mean micellar aggregation numbers of mixed micelles were obtained. In all the cases, the aggregation number is initially reduced with the participation of the cationic surfactant, remaining almost constant and close to the aggregation number of the pure cationic micelles. The local viscosity of pure and mixed micelles was examined by the photophys. response of the hydrophobic probe coumarin 6 solubilized in the micellar medium. The participation of the ionic component induces the formation of a less ordered structure than that of pure nonionic micelles. This effect becomes less pronounced as the chain hydrocarbon length of the co-surfactant increases.

Colloid and Polymer Science published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Synthetic Route of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Sawkar, Anu R. published the artcileGaucher Disease-Associated Glucocerebrosidases Show Mutation-Dependent Chemical Chaperoning Profiles, Category: alcohols-buliding-blocks, the publication is Chemistry & Biology (Cambridge, MA, United States) (2005), 12(11), 1235-1244, database is CAplus and MEDLINE.

Summary: Gaucher disease is a lysosomal storage disorder caused by deficient glucocerebrosidase activity. We have previously shown that the cellular activity of the most common Gaucher disease-associated glucocerebrosidase variant, N370S, is increased when patient-derived cells are cultured with the chem. chaperone N-nonyl-deoxynojirimycin. Chem. chaperones stabilize proteins against misfolding, enabling their trafficking from the endoplasmic reticulum. Herein, the generality of this therapeutic strategy is evaluated with other glucocerebrosidase variants and with addnl. candidate chem. chaperones. Improved chem. chaperones are identified for N370S glucocerebrosidase. Moreover, we demonstrate that G202R, a glucocerebrosidase variant that is known to be retained in the endoplasmic reticulum, is also amenable to chem. chaperoning. The L444P variant is not chaperoned by any of the active site-directed mols. tested, likely because this mutation destabilizes a domain distinct from the catalytic domain.

Chemistry & Biology (Cambridge, MA, United States) published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts