Category: 85618-21-9

Lo Leggio, Leila published the artcileThe Structure and Specificity of Escherichia coli Maltose Acetyltransferase Give New Insight into the LacA Family of Acyltransferases, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Biochemistry (2003), 42(18), 5225-5235, database is CAplus and MEDLINE.

The crystallog. three-dimensional structure of the Escherichia coli maa gene product, previously identified as a maltose O-acetyltransferase (MAT) [Brand, B., and Boos, W. (1991) J. Biol. Chem. 266, 14113-14118] has been determined to 2.15 Å resolution by the single anomalous dispersion method using data from a crystal cocrystd. with trimethyllead acetate. It is shown here that MAT acetylates glucose exclusively at the C6 position and maltose at the C6 position of the nonreducing end glucosyl moiety. Furthermore, MAT shows higher affinity toward artificial substrates containing an alkyl or hydrophobic chain as well as a glucosyl unit. The presence of a long hydrophobic patch near the acceptor site provides the structural explanation for this preference. The three-dimensional structure reveals the expected trimeric left-handed parallel β-helix structure found in all other known hexapeptide repeat enzymes. In particular, the structure shows similarities both overall and at the putative active site to the recently determined structure of galactoside acetyltransferase (GAT), the lacA gene product [Wang, X.-G., Olsen, L. R., and Roderick, S. L. (2002) Structure 10, 581-588]. The structure, together with the new biochem. data, suggests that GAT and MAT are more closely related than previously thought and might have similar cellular functions. However, while GAT is specific for acetylation of galactosyl units, MAT is specific for glucosyl units and is able to acetylate maltooligosaccharides, an important property for biotechnol. applications. Structural differences at the acceptor site reflect the differences in substrate specificity.

Biochemistry published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

McPherson, Alexander published the artcileSearching for silver bullets: An alternative strategy for crystallizing macromolecules, Synthetic Route of 85618-21-9, the publication is Journal of Structural Biology (2006), 156(3), 387-406, database is CAplus and MEDLINE.

Based on a hypothesis that various small mols. might establish stabilizing, intermol., non covalent crosslinks in protein crystals and thereby promote lattice formation, the authors carried out three sep. experiments The authors assessed the impact of 200 chems. on the propensity of 81 different proteins and viruses to crystallize. The experiments were comprised of 18 240 vapor diffusion trials. A salient feature of the experiments was that, aside from the inclusion of the reagent mixes, only two fundamental crystallization conditions were used, 30% PEG 3350, and 50% Tacsimate at pH 7. Overall, 65 proteins (85%) were crystallized Most significant was that 35 of the 65 (54%) crystallized only in the presence of one or more reagent mixes, but not in control samples lacking any additives. Among the most promising types of reagent mixes were those composed of polyvalent, charged groups, such as di and tri carboxylic acids, diamino compounds, mols. bearing one or more sulfonyl or phosphate groups, and a broad range of common biochems., coenzymes, biol. effectors, and ligands. The authors propose that an alternate approach to crystallizing proteins might be developed, which employs a limited set of fundamental crystallization conditions combined with a broad screen of potentially useful small mol. additives.

Journal of Structural Biology published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Synthetic Route of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Patel, Ekta published the artcileAlternative Surfactants for Improved Efficiency of In Situ Tryptic Proteolysis of Fingermarks, Computed Properties of 85618-21-9, the publication is Journal of the American Society for Mass Spectrometry (2015), 26(6), 862-872, database is CAplus and MEDLINE.

Despite recent improvements to in situ proteolysis strategies, a higher efficiency is still needed to increase both the number of peptides detected and the associated ion intensity, leading to a complete and reliable set of biomarkers for diagnostic or prognostic purposes. In the study presented here, an extract of a systematic study is illustrated studying a range of surfactants assisting trypsin proteolytic activity. Method development was trialled on fingermarks; this specimen results from a transfer of sweat from an individual’s fingertip to a surface upon contact. As sweat carries a plethora of biomols., including peptides and proteins, fingermarks are, potentially, a very valuable specimen for non-invasive prognostic or diagnostic screening. A recent study demonstrated the opportunity to quickly detect peptides and small proteins in fingermarks using Matrix Assisted Laser Desorption Ionization Mass Spectrometry Profiling (MALDI MSP). However, intact detection bears low sensitivity and does not allow species identification; therefore, a shotgun proteomic approach was employed involving in situ proteolysis. In fingermarks, further improvements to the existing method can be achieved using MEGA-8 as surfactant in higher percentages as well as combinations of different detergents. Also, for the first time, Rapigest SF, normally used in solution digestions, has been shown to successfully work also for in situ proteolysis. [Figure not available: see fulltext.].

Journal of the American Society for Mass Spectrometry published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Computed Properties of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Dhabaria, Avantika published the artcileA High-Efficiency Cellular Extraction System for Biological Proteomics, Computed Properties of 85618-21-9, the publication is Journal of Proteome Research (2015), 14(8), 3403-3408, database is CAplus and MEDLINE.

Recent developments in quant. high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of the biochem. analyses of cellular reactions, protein-protein interactions, and small-mol.-drug discovery. These approaches depend on cellular proteome extraction that preserves native protein activities. Here, we systematically analyzed mech. methods of cell lysis and phys. protein extraction to identify those that maximize the extraction of cellular proteins while minimizing their denaturation. Cells were mech. disrupted using Potter-Elvehjem homogenization, probe- or adaptive-focused acoustic sonication, and were in the presence of various detergents, including polyoxyethylene ethers and esters, glycosides, and zwitterions. Using fluorescence spectroscopy, biochem. assays, and mass spectrometry proteomics, we identified the combination of adaptive focused acoustic (AFA) sonication in the presence of a binary poloxamer-based mixture of octyl-β-glucoside and Pluronic F-127 to maximize the depth and yield of the proteome extraction while maintaining native protein activity. This binary poloxamer extraction system allowed for native proteome extraction comparable in coverage to the proteomes extracted using denaturing SDS or guanidine-containing buffers, including the efficient extraction of all major cellular organelles. This high-efficiency cellular extraction system should prove useful for a variety of cell biochem. studies, including structural and functional proteomics.

Journal of Proteome Research published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Computed Properties of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Wu, Xiubing published the artcileSynthesis and surface properties of alkyl β-D-thioglucopyranoside, Related Products of alcohols-buliding-blocks, the publication is Journal of Molecular Liquids (2019), 282-289, database is CAplus.

Alkyl thioglycosides are a class of nonionic sugar-based sulfur-containing surfactants and bioreagents. The surfactants 1,2-trans alkyl β-D-thioglucopyranosides with different alkyl chain length (n = 6-12) were stereoselectively prepared by the Helferich method. Their properties including HLB number, logP value, water solubility, foam property, emulsifying property, surface property and thermotropic liquid crystal property were mainly investigated. The results showed that their HLB numbers and water-solubility decreased as the related logP values increased with increasing the alkyl chain length. Alkyl β-D-thioglucosides were already insoluble in water with n = 10. Both β-D-thioglucopyranosides (n = 8, 9) reduced the surface tension of the related aqueous solution to nearly 29 mN.m^-1 at the critical micelle concentration (CMC), they also had excellent foaming ability and foam stability. Nonyl β-D-thioglucopyranoside had good emulsifying properties for both n-octane/water system and toluene/water system. Alkyl β-D-thioglucopyranosides (n = 6-12) were observed to have the thermotropic liquid crystal properties.

Journal of Molecular Liquids published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C22H18O2, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gadre, Dhanesh published the artcileEndotoxin reduction in protein solutions using octyl β-D-1-thioglucopyranoside wash on chromatography media, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Journal of Chromatography A (2018), 49-58, database is CAplus and MEDLINE.

Endotoxins are complex mols. and one of the most challenging impurities requiring separation in biopharmaceutical protein purification processes. Usually these contaminants are cleared during the downstream process, but if endotoxin interacts with the target protein it becomes difficult to remove. In the present study we identified a detergent, octyl-β-D-1-thioglucopyranoside (OTG), that disrupted endotoxin-protein interactions. The integration of this detergent into washes on several chromatog. media was demonstrated to provide a separation tool for decreasing endotoxin from target proteins. This study also examined the mechanism of OTG endotoxin-protein disruption through phase modification incubation and chromatog. studies. The non-ionic OTG wash was shown to break both hydrophobic and electrostatic interactions between the endotoxin and protein. This mechanism contrasts with the breaking of hydrophobic interactions by washing with known endotoxin decreasing Triton X-100 detergent. The difference in mechanisms likely results in the ability of OTG to decrease endotoxin to levels less than those resulting from a detergent wash such as Triton X-100. Finally, we show the impact of the OTG endotoxin removal tool on the biopharmaceutical industry. While maintaining monomer purity and activity levels, endotoxin removal from a fusion protein allowed for decreased background levels in a T cell functional assay. The lowered baseline of T cell responses allowed for more effective detection of mol. interaction with the cells. The detergent wash can be used to both decrease the overall level of endotoxin in a purified protein solution and to enable more effective screening of lead candidate mols.

Journal of Chromatography A published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gonzalez-Ramirez, Luis A. published the artcileInvestigation of the Compatibility of Gels with Precipitating Agents and Detergents in Protein Crystallization Experiments, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Crystal Growth & Design (2008), 8(12), 4291-4296, database is CAplus.

Different types of gels are currently used as crystallization media avoiding convective fluid motion and sedimentation of crystals, two properties that are beneficial when seeking crystals of the highest quality for structural studies. The authors show in this paper the compatibility of the main precipitating agents, buffers, and additives, including some detergents employed in protein crystallization with certain gels, namely, agaroses with two different gelling temperatures, polyacrylamide and silica gels such as tetramethylorthosilicate.

Crystal Growth & Design published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Vichapong, Jitlada published the artcileAlternative spectrophotometric method for determination of bilirubin and urobilinogen in urine samples using simultaneous injection effective mixing flow analysis, Quality Control of 85618-21-9, the publication is Analytical Methods (2013), 5(9), 2419-2426, database is CAplus.

A new approach based on simultaneous injection effective mixing flow anal. (SIEMA) system was developed for the successive spectrophotometric determination of urobilinogen and bilirubin in urinary samples. A 4-channel SIEMA system consisted of a syringe pump, two 5-cross connectors, four holding coils (two of four were used for urobilinogen determination, and other two of four were used for bilirubin determination), five solenoid valves, a mixing coil and a spectrophotometer. For urobilinogen assay, the color development was based on the reaction with p-diethylaminobenzaldehyde (p-DEABA) in the presence of strong hydrochloric acid. On the other hand, bilirubin was measured by the reaction with diazotized sulfanilic acid in the presence of n-octyl-β-d-thioglucoside (OTG) which served as a solubilizing agent to form OTG-azobilirubin. Firstly for bilirubin determination, aliquots of sample and reagent solutions were aspirated into two individual holding coils by a syringe pump, and then the aspirated zones were simultaneously dispensed in the reverse direction to the detector flow cell. Secondary for urobilinogen determination, aliquots of sample and reagent for urobilinogen were aspirated into other two holding coils, followed by the similar spectrophotometric detection. Under optimal conditions, the automated method provided linearity up to 100.0 mg L^-1 for urobilinogen and 5.0 mg L^-1 for bilirubin. The 3σ limit of detections (LODs) for urobilinogen and bilirubin were 1.0 mg L^-1 and 0.003 mg L^-1, resp. The relative standard deviations (RSDs, n = 11) of 30 mg L^-1 urobilinogen and 1.0 mg L^-1 for bilirubin were 1.5% and 1.0%, resp. Sample throughput of the whole assay of both urobilinogen and bilirubin was 30 h^-1. The proposed method can be applied to trace urobilinogen and bilirubin in urine samples and the results are useful for screening diabetic diagnostic.

Analytical Methods published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C6H10O7, Quality Control of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Stuart, Marc C. A. published the artcileTwo distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition are mediated by the packing parameter of phospholipid-detergent systems, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Biochimica et Biophysica Acta, Biomembranes (2007), 1768(11), 2681-2689, database is CAplus and MEDLINE.

The detergent solubilization and reformation of phospholipid vesicles was studied for various detergents. Two distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition were observed by turbidimetry and cryo-electron microscopy. The first mechanism involves fast solubilization of phospholipids and occurs via open vesicular intermediates. The reverse process, micelle-to-vesicle transition, mimics the vesicle-to-micelle transition. In the second mechanism the solubilization is a slow process that proceeds via micelles that pinch off from closed vesicles. During vesicle reformation, the micelle-to-vesicle transition, a large number of densely packed multilamellar vesicles are formed. The route used, for solubilization and reformation, by a given detergent-phospholipid combination is critically dependent on the overall packing parameter of the detergent-saturated phospholipid membranes. By a change of the overall packing parameter the solubilization and or reformation mechanism could be changed. All five detergents tested fit within the proposed model. With two detergents the mechanism could be changed by changing the phospholipid composition or the medium conditions.

Biochimica et Biophysica Acta, Biomembranes published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C12H10F2Si, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Lopez de Laorden, Carlos published the artcileNanostructured Indium Tin Oxide Slides for Small-Molecule Profiling and Imaging Mass Spectrometry of Metabolites by Surface-Assisted Laser Desorption Ionization MS, HPLC of Formula: 85618-21-9, the publication is Analytical Chemistry (Washington, DC, United States) (2015), 87(1), 431-440, database is CAplus and MEDLINE.

Due to their elec. conductivity and optical transparency, slides coated with a thin layer of indium tin oxide (ITO) are the standard substrate for protein imaging mass spectrometry on tissue samples by MALDI-TOF MS. The authors have now studied the radiofrequency magnetron sputtering deposition parameters to prepare ITO thin films on glass substrates with the required nanometric surface structure for their use in the matrix-free imaging of metabolites and small-mol. drugs, without affecting the transparency required for classical histol. The custom-made surfaces were characterized by at. force microscopy, SEM, ellipsometry, UV, and laser desorption ionization MS (LDI-MS) and employed for the LDI-MS-based anal. of glycans and druglike mols., the quantification of lactose in milk by isotopic dilution, and metabolite imaging on mouse brain tissue samples.

Analytical Chemistry (Washington, DC, United States) published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, HPLC of Formula: 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts