Category: 85618-21-9

Antharavally, Babu S. published the artcileEfficient removal of detergents from proteins and peptides in a spin column format, Synthetic Route of 85618-21-9, the publication is Analytical Biochemistry (2011), 416(1), 39-44, database is CAplus and MEDLINE.

Detergents are commonly used in protein-chem. protocols and may be necessary for protein extraction, solubilization, and denaturation; however, their presence interferes with many downstream anal. techniques, including mass spectrometry (MS). To enable downstream anal., it is critical to remove unbound detergents from protein and peptide samples. In this study, the authors describe a high-performance resin that offers exceptional detergent removal for proteins and peptides. When used in a spin column format, this resin dramatically improves protein and peptide MS results by more than 95% removal of 1-5% detergents, including SDS, sodium deoxycholate, Chaps, Triton X-100, Triton X-114, NP-40, Brij-35, octyl glucoside, octyl thioglucoside, and lauryl maltoside, with high recovery of proteins and peptides. Postcolumn liquid chromatog.-tandem MS (LC-MS/MS) anal. of trypsin digests of bovine serum albumin (BSA) and HeLa cell lysate revealed excellent sequence coverage, indicating successful removal of detergent from the peptides. Matrix-assisted laser desorption/ionization (MALDI)-MS anal. of unprocessed and processed samples further confirmed efficient removal of detergents. The advantages of this method include speed (<15 min), efficient detergent removal, and high recovery of proteins and peptides.

Analytical Biochemistry published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Synthetic Route of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Jana, Rabindranath published the artcileStructural transition dynamics of biologically active flavins in alkylglucoside surfactants aggregates, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (2020), 117346, database is CAplus and MEDLINE.

The photophysics and structural transition dynamics of a bio-active flavin lumichrome (LM) entrapped in two sugars based neutral surfactants are reported. Sugar-based surfactants, which were used for research purpose are potential environmentally friendly, green alternative amphiphilic surface active substance compared to other kinds of common surfactants. Here, two alkyl glucoside surfactants n-octyl-β-D-glucopyranoside (OBG) and n-octyl-β-D-thioglucopyranoside (OBTG) were used. This work was carried out by using steady-state absorption and fluorescence emission spectroscopy along with time-resolved fluorescence emission techniques. Photophysics of LM was modulated several folds in these two sugar-based neutral micelles. LM exhibits excitation and emission wavelength dependent fluorescence properties in these two sugars based neutral micelles. LM confined in the micellar environments exhibited structural transition dynamism, i.e. different kinds of conformers are equilibrated. Herein, different conformers of LM are identified and explained with the help of spectroscopic methods. From the fluorescence anisotropy measurement, the rotational relaxation time of LM in OBG micelle was more compared to that in OBTG micelle, which indicates that the LM mol. faced much more constrained environment in OBG micellar media.

Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Reading, Eamonn published the artcileThe Role of the Detergent Micelle in Preserving the Structure of Membrane Proteins in the Gas Phase, Computed Properties of 85618-21-9, the publication is Angewandte Chemie, International Edition (2015), 54(15), 4577-4581, database is CAplus and MEDLINE.

Despite the growing importance of the mass spectrometry of membrane proteins, it is not known how their transfer from solution into vacuum affects their stability and structure. To address this we have carried out a systematic investigation of ten membrane proteins solubilized in different detergents and used mass spectrometry to gain physicochem. insight into the mechanism of their ionization and desolvation. We show that the chem. properties of the detergents mediate the charge state, both during ionization and detergent removal. Using ion mobility mass spectrometry, we monitor the conformations of membrane proteins and show how the surface charge d. dictates the stability of folded states. We conclude that the gas-phase stability of membrane proteins is increased when a greater proportion of their surface is lipophilic and is consequently protected by the phys. presence of the micelle.

Angewandte Chemie, International Edition published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Computed Properties of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Koroleva, Sofia V. published the artcileMolecular Thermodynamic Modeling of the Specific Effect of Salt on the Aggregation of Nonionic Surfactants, Recommanded Product: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Journal of Chemical & Engineering Data (2020), 65(3), 987-992, database is CAplus.

The specific chem. of added salt has a strong effect on the aggregation of surfactants. Although the mol. mechanism of this effect is still debated in the literature and there is no generally accepted quant. theory, substantial progress has recently been achieved in the mol. thermodn. modeling of ion-specific effects for solutions of ionic surfactants. In this work, we extend our previous aggregation model of ionic surfactants to solutions of nonionic surfactants in the presence of salts. Within this model, the specificity of ions is reflected by the difference in ionic diameters, the dispersion interaction with the micelle, and an effective parameter δ_± that takes into account the hydration/dehydration of an ion in the micellar corona and is specific to every ion-surfactant head pair. The effect of specific salt on the hydrophobic contribution to the aggregation free energy is described via the Setchenov salting-out constants We apply the model for sugar-based surfactants: n-alkyl glucosides and N-acyl-N-methylglucosides. This choice is motivated by the importance of this family of surfactants in biotechnol. We report the set of model parameters, including the Setchenov constants, for the surfactants in this family in combination with a number of 1:1 salts and illustrate good performance of the model in the description of the specific effect of added salt on the critical micelle concentration (CMC) and the growth of micellar aggregates.

Journal of Chemical & Engineering Data published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Recommanded Product: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

van Dyck, Jeroen F. published the artcileNative mass spectrometry for the characterization of structure and interactions of membrane proteins, SDS of cas: 85618-21-9, the publication is Methods in Molecular Biology (New York, NY, United States) (2017), 205-232, database is CAplus and MEDLINE.

Over the past years, native mass spectrometry and ion mobility have grown into techniques that are widely applicable to the study of aspects of protein structure. More recently, it has become apparent that this approach provides a very promising avenue for the investigation of integral membrane proteins in lipid or detergent environments. In this chapter, we discuss applications of native mass spectrometry and ion mobility in membrane protein research-what is important to take into consideration when working with membrane proteins, and what the requirements are for sample preparation for native mass spectrometry. Furthermore, we will discuss the types of information provided by the measurements, including the oligomeric state, subunit composition and stoichiometry, interactions with detergents or lipids, conformational transitions, and the binding and structural effect of ligands and drugs.

Methods in Molecular Biology (New York, NY, United States) published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C5H10O2S, SDS of cas: 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gonzalez-Gil, Lorena published the artcileBiotransformation of organic micropollutants by anaerobic sludge enzymes, Quality Control of 85618-21-9, the publication is Water Research (2019), 202-214, database is CAplus and MEDLINE.

Biotransformation of organic micropollutants (OMPs) in wastewater treatment plants ultimately depends on the enzymic activities developed in each biol. process. However, few research efforts have been made to clarify and identify the role of enzymes on the removal of OMPs, which is an essential knowledge to determine the biotransformation potential of treatment technologies. Therefore, the purpose of the present study was to investigate the enzymic transformation of 35 OMPs under anaerobic conditions, which have been even less studied than aerobic systems. Initially, 13 OMPs were identified to be significantly biotransformed (>20%) by anaerobic sludge obtained from a full-scale anaerobic digester, predestining them as potential targets of anaerobic enzymes. Native enzymes were extracted from this anaerobic sludge to perform transformation assays with the OMPs. In addition, the effect of detergents to recover membrane enzymes, as well as the effects of cofactors and inhibitors to promote and suppress specific enzymic activities were evaluated. In total, it was possible to recover enzymic activities towards 10 out of these 13 target OMPs (acetyl-sulfamethoxazole and its transformation product sulfamethoxazole, acetaminophen, atenolol, clarithromycin, citalopram, climbazole, erythromycin, and terbutryn, venlafaxine) as well as towards 8 non-target OMPs (diclofenac, iopamidol, acyclovir, acesulfame, and 4 different hydroxylated metabolites of carbamazepine). Some enzymic activities likely involved in the anaerobic biotransformation of these OMPs were identified. Thereby, this study is a starting point to unravel the still enigmatic biotransformation of OMPs in wastewater treatment systems.

Water Research published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Quality Control of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Erkan, Sultan published the artcileStructural, spectral characterization and molecular docking analyses of mer-ruthenium (II) complexes containing the bidentate chelating ligands, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (2020), 117399, database is CAplus and MEDLINE.

The authors analyzed some monofunctional Ru (II) complexes containing chlorine, bromine and fluorine atoms around the central atom. The best calculation level among HF, B3LYP and M062X methods for [Ru (Cl-Ph-tpy)(N-N)X]⁺ (X = F, Cl, Br) was determined in the light of Benchmark anal. and according to this anal. results, the best level is shown as B3LYP-LANL2DZ/6-31G(d). In addition to this, the spectroscopic data (IR, NMR and UV-visible) were also obtained in agreement with exptl. results. The tendency of anticancer activity and structural activity relation (SAR) parameters are predicted with some quantum chem. methods. Surface and contour diagrams, as well as electron densities on mentioned complexes were interpreted through theor. obtained results. Finally, the anticancer activity tendency of the relevant complexes on the human cervical carcinoma cell line (ID: 1 M17) is supported by mol. docking calculations

Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Name: (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Tran, Chieu D. published the artcileChiral separation of amino acids by capillary electrophoresis with octyl-β-thioglucopyranoside as chiral selector, Synthetic Route of 85618-21-9, the publication is Journal of Chromatography A (2002), 978(1-2), 221-230, database is CAplus and MEDLINE.

1-S-Octyl-β-D-thioglucopyranoside (OTG) was evaluated as a chiral selector for the separation of dansylamino acids by capillary electrophoresis. Enantiomeric separations of the amino acids can be accomplished by judiciously adjusting the pH of the solution and the concentration of OTG. Better separation can be achieved, however, when OTG was used, not as a sole chiral selector but rather together with sodium dodecyl sulfate (SDS) and cyclodextrin (CD). Not only can this OTG-SDS-CD system provide better separation than OTG alone but also it can sep. compounds which cannot be separated when only CD or CD and SDS were used. All amino acids were baseline separated with this OTG-SDS-CD system at optimal conditions.

Journal of Chromatography A published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C8H15ClN2, Synthetic Route of 85618-21-9.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Santonicola, Gabriella M. published the artcileMixtures of n-Octyl-β-D-glucoside and Triethylene Glycol Mono-n-octyl Ether: Phase Behavior and Micellar Structure near the Liquid-Liquid Phase Boundary, Related Products of alcohols-buliding-blocks, the publication is Langmuir (2005), 21(22), 9955-9963, database is CAplus and MEDLINE.

The phase behavior and microstructure of aqueous mixtures of n-octyl-β-D-glucoside (C₈βG₁) and triethylene glycol mono-n-octyl ether (C₈E₃) is presented. C₈βG₁ forms a one-phase micellar solution in water at surfactant concentrations up to 60 wt %, whereas mixtures with C₈E₃ show a liquid-liquid phase transition at low surfactant concentration The position of this phase boundary for mixtures can be rationally shifted in the temperature-composition window by altering the ratio of the two surfactants. Small-angle neutron scattering is used to determine the size and shape of the mixed micelles and to characterize the nature of the fluctuations near the cloud point of the micellar solutions The C₈βG₁/C₈E₃ solutions are characterized by concentration fluctuations that become progressively stronger upon approach to the liquid-liquid phase boundary, whereas micellar growth is negligible. Such observations confirm previous views of the role of the surfactant phase boundary in tuning attractive micellar interactions, which can be used effectively to change the nature and strength of interparticle interactions in colloidal dispersions. Colloidal silica particles were then added to these surfactant mixtures and were found to aggregate at conditions near the cloud point. This finding is relevant to current strategies for protein crystallization

Langmuir published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C14H28O5S, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Westerman, Larry E. published the artcileLiposomes composed of reconstituted membranes for induction of tumor-specific immunity, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, the publication is Methods in Enzymology (2003), 118-127, database is CAplus and MEDLINE.

The methods to generate exptl. cell-free cancer vaccines consisting of reconstituted tumor membrane liposomes with incorporated immunomodulatory mols. are described. Using a detergent dialysis technique, tumor cell membranes can be reassembled with the addition of purified membranes included to promote tumor-specific immune responses. A major advantage of this approach is that immunostimulatory proteins can be presented on a membrane like structure that resembles the tumor cell plasma membrane.

Methods in Enzymology published new progress about 85618-21-9. 85618-21-9 belongs to alcohols-buliding-blocks, auxiliary class Tetrahydropyran,Chiral,sulfides,Alcohol, name is (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C10H12O5, Application of (2R,3S,4S,5R,6S)-2-(Hydroxymethyl)-6-(octylthio)tetrahydro-2H-pyran-3,4,5-triol.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts