Category: 70539-42-3

Marasco, Wayne A. published the artcileCovalent affinity labeling, detergent solubilization, and fluid-phase characterization of the rabbit neutrophil formyl peptide chemotaxis receptor, Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biochemistry (1985), 24(9), 2227-36, database is CAplus and MEDLINE.

The formyl peptide chemotaxis receptor of rabbit neutrophils and purified rabbit neutrophil plasma membranes was identified by several affinity labeling techniques: (1) covalent affinity crosslinking of N-formyl-Nle-Leu-Phe-Nle-¹²⁵I-Tyr-Lys (¹²⁵I-hexapeptide) to the membrane-bound receptor with either di-Me suberimidate or ethylene glycol bis(succinimidyl succinate) and (2) photoactivation of N-formyl-Nle-Leu-Phe-Nle-¹²⁵I-Tyr-Lys-N^ε-6-[(4-azido-2-nitrophenyl)amino]hexanoate (¹²⁵I-PAL). These techniques specifically identify the receptor as a polypeptide that migrates as a broad band on SDS-polyacrylamide electrophoresis, with a mol. weight (M_r) 50,000-65,000. The receptor was solubilized in active form from rabbit neutrophil membranes by using the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and digitonin, and from whole cells with CHAPS. Chemotaxis receptor activity was measured by the ability of the solubilized membrane material to bind ¹²⁵I-hexapeptide or N-formyl-Met-Leu-[³H]Phe with gel filtration or rapid filtration through poly(ethylenimine) (PEI)-treated filters as assay systems. ¹²⁵I-PAL was specifically crosslinked to the same mol. weight material in the CHAPS and digitonin solubilized extract, but no specific labeling of the receptor was seen when membranes were extracted with Nonidet P-40 and Triton X-100. Therefore, although a large number of detergents are able to solubilize the receptor, apparently some detergents release the receptor in an inactive form. The ligand binding characteristics of formyl-Met-Leu-[³H]Phe to the CHAPS-solubilized receptor shared properties with the membrane-bound formyl peptide receptor, both of which showed curvilinear, concave-upward Scatchard plots. Computer curve fitting with the program NONLIN and statistical analyses of the binding data indicated that for both the membrane-bound and solubilized receptors a 2-saturable sites model fitted the data significantly better than did a 1-saturable site model. The characteristics of the 2-saturable sites model for the soluble receptor were: a high-affinity site with a K_D value of 1.25 nM and a low-affinity site with a K_D value of 19.77 nM. A total of 35% of the 2 sites detected was of the higher affinity. A Hill coefficient of 0.61 was observed

Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Safety of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Cleeter, Michael W. J. published the artcileChemical crosslinking of mitochondrial NADH dehydrogenase from bovine heart, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biochemical Journal (1985), 227(2), 467-74, database is CAplus and MEDLINE.

The structure of bovine heart mitochondrial NADH dehydrogenase was investigated by using 2 cleavable crosslinking agents, disuccinimidyl tartrate and (ethylene glycol)yl bis(succinimidyl succinate). Crosslinking was analyzed primarily by immunoblotting to detect products containing subunits of the Fe-protein fraction from chaotropic resolution of the enzyme, namely those of 75, 49, 30, and 13 kilodaltons. Crosslinks were identified between these 4 subunits, from these subunits to the largest subunit of the flavoprotein fraction (which contains the active site for NADH), and from these subunits to polypeptides in the hydrophobic shell (which surrounds the hydrophilic Fe-protein and flavoprotein fractions).

Biochemical Journal published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Higuchi, Masahiro published the artcileModulation of two forms of tumor necrosis factor receptors and their cellular response by soluble receptors and their monoclonal antibodies, Formula: C18H20N2O12, the publication is Journal of Biological Chemistry (1992), 267(29), 20892-9, database is CAplus and MEDLINE.

Recently, two different receptors for human tumor necrosis factor (TNF) with mol. masses of 60 kDa (p60) and 80 kDa (p80) have been identified. This report investigated the effect of the soluble forms of these receptors and monoclonal antibodies against them on ligand interaction, receptor down-regulation, and mediation of cellular response in U-937 cells. The results indicate that p60 and p80 constitute 20-30 and 60-80% of the total TNF-binding sites on U-937 cells, resp. However, by crosslinking, only the p80 form of the receptor could be detected. In contrast to unlabeled TNF, the anti-p60 and anti-p80 antibodies together only partially inhibited ligand binding, and this inhibition was not additive. Lack of additive inhibition of binding was not due to stereochem. hindrance. TNF binding to cells can be completely displaced by soluble forms of either the p60 or p80 receptor. However, 100-fold more of the p80 than the p60 form of the soluble receptor is needed for equivalent displacement. Under optimum conditions, TNF and the anti-p80 and anti-p60 antibodies down-regulated 30, 80, and 20% of the TNF receptors, resp. The anti-p60 and anti-p80 antibodies down-regulated not only their own receptors, but also reciprocal receptors, suggesting a cross-communication between the p60 and p80 forms of the TNF receptor. In spite of inhibiting as much as 80% of TNF binding, none of the receptor antibodies significantly inhibited the cytotoxic response to TNF in U-937 cells. Soluble forms of both receptors, however, completely abrogated the cellular response to TNF. Thus, these results indicate that the antibodies against both receptors together inhibit the majority of the receptor-ligand interaction without any significant effect on the biol. response to TNF.

Journal of Biological Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Stauber, Gregory B. published the artcileCharacterization and affinity cross-linking of receptors for human recombinant lymphotoxin (tumor necrosis factor-β) on a human histiocytic lymphoma cell line, U-937, COA of Formula: C18H20N2O12, the publication is Journal of Biological Chemistry (1989), 264(6), 3573-6, database is CAplus and MEDLINE.

Recombinant human lymphotoxin (rhLT) was purified and used to examine its receptors on the human histiocytic lymphoma cell line U-937. The rhLT was radioiodinated by the IODO GEN method to a specific activity of 60 μCi/μg. The specific binding reached a plateau within 10, 60, and 180 min at 37, 23, and 4°, resp. Scatchard anal. revealed the presence of a single class of high affinity receptors with an apparent K_d of 0.6 nm and a capacity of 33,000 binding sites/cell. The binding of ¹²⁵I-labeled rhLT to U-937 cells was inhibited by excess unlabeled rhLT or recombinant human tumor necrosis factor-α(rhTNF), suggesting a common receptor for both mols. The crosslinking of the receptor to rhLT revealed 2 distinct bands at approx. mol. masses of 100,000 and 120,000 daltons. Affinity crosslinking of U-937 cells with ¹²⁵I-labeled rhTNF, however, provided only a single band with a mol. mass of about 100,000 daltons. Thus, the manner in which rhLT interacts with its receptor is perhaps somewhat different from that of rhTNF.

Journal of Biological Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C16H25BO2, COA of Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts