Category: 70539-42-3

Subramanian, R. published the artcileTissue distribution of indium-111-labeled human monoclonal antibody (16.88) in nude mice bearing tumor xenografts: effect of diester linkage, COA of Formula: C18H20N2O12, the publication is Antibody, Immunoconjugates, and Radiopharmaceuticals (1990), 3(2), 127-36, database is CAplus.

A human IgM anticolorectal monoclonal antibody 16.88 was coupled to DTPA using a diester linkage in an attempt to reduce retention of radioactivity in normal organs of animals receiving the ¹¹¹In-labeled conjugate. For comparison, a 16.88-DTPA conjugate with a peptide linkage was also prepared The diester and peptide conjugates contained 3.4 and 4.3 mols. of DTPA per 16.88, resp., and their immunoreactivity was >90% as assessed by a direct-cell binding assay. Biodistribution studies in nude mice bearing THO human colon tumor xenografts indicated that diester linked conjugate was retained to a lesser extent in tumor and normal organs such as liver and kidney as compared to the peptide conjugate. The clearance of the diester conjugate from blood, however, was much faster than that of the peptide conjugate. This resulted in 2-fold higher tumor-to-serum ratio for the diester conjugate than for the peptide conjugate.

Antibody, Immunoconjugates, and Radiopharmaceuticals published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C19H21N3O3S, COA of Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Patil, Ujwal S. published the artcileCleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins, Quality Control of 70539-42-3, the publication is Analytical Biochemistry (2015), 18-20, database is CAplus and MEDLINE.

To study the solvent-exposed lysine residues of peptides/proteins, we previously reported disulfide-linked N-hydroxysuccinimide ester-modified silica-coated iron oxide magnetic nanoparticles (NHS-SS-SiO₂@Fe₃O₄ MNPs). The presence of a disulfide bond in the linker limits the use of disulfide reducing agent during protein digestion and allows unwanted disulfide formation between the MNPs and protein. In the current work, the disulfide bond was replaced with a cleavable ester group to synthesize NHS ester-modified SiO₂@Fe₃O₄ MNPs. Use of the cleavable ester group provides an improved method for protein labeling and allows the use of disulfide reducing agents during protein digestion.

Analytical Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Quality Control of 70539-42-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Petrotchenko, Evgeniy V. published the artcileIsotopically coded cleavable cross-linker for studying protein-protein interaction and protein complexes, SDS of cas: 70539-42-3, the publication is Molecular and Cellular Proteomics (2005), 4(8), 1167-1179, database is CAplus and MEDLINE.

An emerging approach for studying protein-protein interaction in complexes is the combination of chem. crosslinking and mass spectrometric anal. of the cross-linked peptides (cross-links) obtained after proteolysis of the complex. This approach, however, has several challenges and limitations, including the difficulty of detecting the cross-links, the potential interference from noninformative “cross-linked peptides” (dead end and intrapeptide cross-links), and unambiguous identification of the cross-links by mass spectrometry. Thus, the authors have synthesized an isotopically coded ethylene glycol bis(succinimidylsuccinate) derivate (D₁₂-EGS), which contains 12 deuterium atoms for easy detection of cross-links when applied in a 1:1 mixture with its H₁₂ counterpart and is also cleavable for releasing the cross-linked peptides allowing unambiguous identification by MS sequencing. Moreover, hydrolytic cleavage permits rapid distinguishing between different types of cross-links. Cleavage of a dead end cross-link produces a doublet with peaks 4.03 Da apart, with the lower peak appearing at a mol. mass 162 Da lower than the mass of the H₁₂ form of the original cross-linked peptide. Cleavage of an intrapeptide cross-link leads to a doublet 8.05 Da apart and 62 Da lower than the mol. mass of the H₁₂ form of the original cross-linked peptide. Cleavage of an interpeptide cross-link forms a pair of 4.03-Da doublets, with the lower mass member of each pair each shifted up from its unmodified mol. weight by 82 Da because of the attached portion of the cross-linker. All of this information has been incorporated into a software algorithm allowing automatic screening and detection of cross-links and cross-link types in matrix-assisted laser desorption/ionization mass spectra. In summary, the ease of detection of these species through the use of an isotopically coded cleavable cross-linker and the authors’ software algorithm, followed by mass spectrometric sequencing of the cross-linked peptides after cleavage, has been shown to be a powerful tool for studies of multi-component protein complexes.

Molecular and Cellular Proteomics published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, SDS of cas: 70539-42-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Izumi, Masayuki published the artcileMannose-BSA Conjugates: Comparison Between Commercially Available Linkers in Reactivity and Bioactivity, Formula: C18H20N2O12, the publication is Journal of Carbohydrate Chemistry (2003), 22(5), 317-329, database is CAplus.

Mannosyl ethanolamine and BSA were conjugated together by their amino groups with various homobifunctional cross-linker reagents: disuccinimidyl carbonate (DSC), disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS), ethylene glycolbis(succinimidyl-succinate) (EGS), 1,5-difluoro-2,4-dinitrobenzene (DFDNB), di-Et squarate (DES), and thiophosgene (TP). The resulting mannose-BSA conjugates were subjected to an enzyme-linked lectin assay (ELLA)to investigate their affinity to Con A (ConA). With these results, the seven linkers were evaluated on the basis of five criteria, i.e., cost, reactivity, sugar loading, homogeneity, and affinity to ConA. Thus, DSS, DFDNB, and DES seemed to have advantages over the other crosslinking reagents.

Journal of Carbohydrate Chemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Formula: C18H20N2O12.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

O’Brien, Anne Marie published the artcileLocation of crosslinks in chemically stabilized horseradish peroxidase: implications for design of crosslinks, Related Products of alcohols-buliding-blocks, the publication is Biotechnology and Bioengineering (2001), 76(4), 277-284, database is CAplus and MEDLINE.

The bifunctional compound, ethylene-glycol bis(N-hydroxysuccinimidylsuccinate) (EGNHS), stabilizes horseradish peroxidase C (HRP) by reaction with the enzyme’s lysine residues. In this study we compare native and modified HRP by proteolytic fragmentation, peptide sequencing, and mass spectroscopy, and identify the sites of modification. Most significantly, EGNHS is shown to form a crosslink between Lys232 and Lys241 of HRP and modifies Lys174 without formation of a crosslink. These findings are in agreement with the lysine side-chain reactivities predicted from the surface accessibility of the amino groups, and the maximal span of 16 Å of the EGNHS crosslinker.

Biotechnology and Bioengineering published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

O’Brien, Anne Marie published the artcileDye bleaching and phenol precipitation by phthalic anhydride-modified horseradish peroxidase, Name: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Journal of Chemical Technology & Biotechnology (2000), 75(5), 363-368, database is CAplus.

The phenol precipitation and dye bleaching capabilities of phthalic anhydride-modified horseradish peroxidase C (PA-HRP) were compared with those of native HRP C and ethylene glycol bis(succinic acid N-hydroxysuccinimide ester)-modified HRP (EG-HRP) reported previously. The removal efficiency (percentage of phenol removed from solution under exptl. conditions) was determined for native HRP and both modified forms. Removal efficiencies at 37° were very similar, with >95% removal in each case. Removal efficiencies were less at 70° overall (range 25-45%), but PA-and EG-HRP removed up to 50% more phenol than native HRP. The three HRP forms showed similar dye bleaching performance at 37° in the presence of H₂O₂ and accelerators (up to 86% color removal). PA- and EG-HRP showed slightly greater bleaching abilities at 65° than native HRP for some of the dye/accelerator combinations tested. Modified HRPs performed better in 40% (volume/volume) mixtures of dioxane or DMF.

Journal of Chemical Technology & Biotechnology published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Name: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Colleary, Sandra published the artcileStability and catalytic properties of chemically modified pig trypsin, Related Products of alcohols-buliding-blocks, the publication is Biocatalysis and Biotransformation (2009), 27(5-6), 309-317, database is CAplus.

Pig trypsin was chem. modified with the bifunctional compound ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) to yield EG-trypsin. EG-trypsin showed greater thermal stability (100% active beyond 100 min at 55°C; native only 53% active at 100 min) together with slightly increased tolerance toward some organic solvents. Arg/Lys hydrolysis ratio changed little. Esterase/amidase activity ratio of EG-trypsin in buffer was 11-fold greater than that of native pig trypsin, but 5-fold less in 30% volume/volume acetonitrile. In buffer, EG-trypsin synthesized the dipeptide benzoyl-Arg-Leu-NH₂ at a 3-fold higher rate than native trypsin, but native trypsin outperformed EG-trypsin in 30% volume/volume acetonitrile.

Biocatalysis and Biotransformation published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Kuge, Souichi published the artcileIdentification of peptide fragments chemically cross-linked in cytochrome c oxidase from thermophilic Bacillus PS3, Application of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biochemistry and Molecular Biology International (1996), 38(1), 181-188, database is CAplus and MEDLINE.

In order to study steric arrangement of subunits in cytochrome c oxidase isolated from thermophilic Bacillus PS3, we developed a simple procedure including chem. crosslinking, two consecutive runs of electrophoresis, proteolytic digestion, and peptide sequencing for simultaneous identification of two cross-linked fragments. By this procedure, the cytochrome c domain of subunit 2 was found cross-linked to the C-terminal region of subunit 1 including two hydrophobic transmembrane segments, suggesting that these two regions were located close each other. The present simple procedure might be applicable to proteins whose crystal structures are not revealed.

Biochemistry and Molecular Biology International published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Application of Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Yu, Marcella published the artcileReversible pH Lability of Cross-linked Vault Nanocapsules, HPLC of Formula: 70539-42-3, the publication is Nano Letters (2008), 8(10), 3510-3515, database is CAplus and MEDLINE.

Vaults are ubiquitous, self-assembled protein nanocapsules with dimension in the sub-100 nm range that are conserved across diverse phyla from worms to humans. Their normal presence in humans at a copy number of over 10 000/cell makes them attractive as potential drug delivery vehicles. Toward this goal, bifunctional amine-reactive reagents are shown to be useful for the reversible crosslinking of recombinant vaults such that they may be closed and opened in a controllable manner.

Nano Letters published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C26H41N5O7S, HPLC of Formula: 70539-42-3.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Potschka, Martin published the artcileThe two coiled coils in the isolated rod domain of the intermediate filament protein desmin are staggered. A hydrodynamic analysis of tetramers and dimers, Related Products of alcohols-buliding-blocks, the publication is European Journal of Biochemistry (1990), 190(3), 503-8, database is CAplus and MEDLINE.

Desmin protofilaments and the proteolytically derived α-helical rod domain have been characterized by high-resolution gel permeation chromatog. (GPC) using columns calibrated for the determination of viscosity radii. Addnl. characterization by chem. crosslinking and the determination of sedimentation values allowed the calculation of the mol. dimensions of the mol. species isolated. In dilute buffers GPC separated desmin rod preparations into 2 complexes: a dimer species (single coiled coil) with a length of 50 nm and a tetramer species (2 coiled coils) with a length of 65 nm. Thus the 2 coiled coils in the tetramer are staggered by ∼15 nm. The hydrodynamically derived lengths of the rod dimer and tetramer are supported by electron microscopy after metal shadowing. The hydrodynamic properties of desmin protofilaments follow that of the rod tetramer. The present results explain the inhomogeneity of mols. encountered in previous electron microscopical analyses.

European Journal of Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts