Category: 59-23-4

Huang, Chao published the artcileA pectic polysaccharide from water decoction of Xinjiang Lycium barbarum fruit protects against intestinal endoplasmic reticulum stress, Computed Properties of 59-23-4, the main research area is Lycium fruit pectic polysaccharide intestine endoplasmic reticulum stress China; ER stress; Lycium barbarum; Pectic polysaccharide.

Neutral polysaccharides from Ningxia L. barbarum fruit have been reported with immunomodulatory and antioxidative biol. activities. Few studies on pectic polysaccharides have been reported, especially not from the Xinjiang L. barbarum. In the present study, a pectic polysaccharide, XLBP-I-I, was obtained from water decoction of Xinjiang L. barbarum using anion exchange chromatog. and gel filtration. The results from methanolysis, methylation, FT-IR and NMR experiments indicated that XLBP-I-I was a typical pectic polysaccharide. In vitro assay showed that XLBP-I-I could reduce the ER stress and UPR in tunicamycin insult IPEC-J2 cells, and further protect IPEC-J2 cells against apoptosis induced by ER stress. These results reveal a new perspective for pectic L. barbarum polysaccharides on intestine ER stress, and this elicited interests for its further applications.

International Journal of Biological Macromolecules published new progress about Apoptosis. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Computed Properties of 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Wang, Liying published the artcileDevelopment of sugarcane resource for efficient fermentation of exopolysaccharide by using a novel strain of Kosakonia cowanii LT-1, Synthetic Route of 59-23-4, the main research area is sugarcane fermentation exopolysaccharide Kosakonia cowanii; Exopolysaccharide; Kosakonia cowanii LT-1; Non-food fermentation; Sugarcane juice; Transcriptome sequencing.

This work focuses on the development of non-food fermentation for the cost-effective biosynthesis of exopolysaccharide (EPS) by using a new strain of Kosakonia cowanii LT-1. This novel strain more efficiently utilizes sucrose for EPS production than other glycosyl donors. Comparative transcriptomic anal. is used to understand EPS synthesis promotion and the effects of sucrose on EPS biosynthesis. We speculate that ATP-binding cassette transporter, phosphotransferase, and two-component systems may be the most essential factors for EPS biosynthesis. The enhanced oxidative phosphorylation increases the synthesis rate of ATP to satisfy the energy demands for EPS production with sucrose as the substrate. Sugarcane juice, a cheap raw material, could improve the EPS yield in batch fermentation and achieve approx. 29.66% cost savings for substrate. Our work presents a promising non-food fermentation approach for the synthesis of high-value industrial products.

Bioresource Technology published new progress about Fermentation. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Synthetic Route of 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Lu, Grace published the artcileProfiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis, Recommanded Product: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is IgG glycan profiling lectin capillary nanogel electrophoresis.

Glycosylated human IgG contains fucosylated biantennary N-glycans with different modifications including N-acetylglucosamine, which bisects the mannose core. Although only a limited number of IgG N-glycan structures are possible, human IgG N-glycans are predominantly biantennary and fucosylated and contain varying levels of α2-6-linked sialic acid, galactose, and bisected N-acetylglucosamine. Monitoring the relative abundance of bisecting N-acetylglucosamine is relevant to physiol. processes. A rapid, inexpensive, and automated method is used to successfully profile N-linked IgG glycans and is suitable to distinguish differences in bisection, galactosylation, and sialylation in N-glycans derived from different sources of human IgG. The separation is facilitated with self-assembled nanogels that also contain a single stationary zone of lectin. When the lectin specificity matches the N-glycan, the peak disappears from the electropherogram, identifying the N-glycan structure. The nanogel electrophoresis generates separation efficiencies of 500 000 plates and resolves the positional isomers of monogalactosylated biantennary N-glycan and the monogalactosylated bisected N-glycan. Aleuria aurantia lectin, Erythrina cristagalli lectin (ECL), Sambucus nigra lectin, and Phaseolus vulgaris Erythroagglutinin (PHA-E) are used to identify fucose, galactose, α2-6-linked sialic acid, and bisected N-acetylglucosamine, resp. Although PHA-E lectin has a strong binding affinity for bisected N-glycans that also contain a terminal galactose on the α1-6-linked mannose branch, this lectin has lower affinity for N-glycans containing terminal galactose and for agalactosylated bisected biantennary N-glycans. The lower affinity to these motifs is observed in the electropherograms as a change in peak width, which when used in conjunction with the results from the ECL lectin authenticates the composition of the agalactosylated bisected biantennary N-glycan. For runs performed at 17°, the precision in migration time and peak area was less than or equal to 0.08 and 4% relative standard deviation, resp. The method is compatible with electrokinetic and hydrodynamic injections, with detection limits of 70 and 300 pM, resp.

Analytical Chemistry (Washington, DC, United States) published new progress about Aleuria aurantia. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Recommanded Product: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Miyamoto, Sachiko published the artcileCase of de novo splice site variant in SLC35A2 showing developmental delays, spastic paraplegia, and delayed myelination, Synthetic Route of 59-23-4, the main research area is SLC35A2 spastic paraplegia delayed myelination congenital disorder glycosylation; SLC35A2 ; congenital disorders of glycosylation; delayed myelination; spastic paraplegia; splice site variant.

Background : Congenital disorders of glycosylation (CDGs) are genetic diseases caused by pathogenic variants of genes involved in protein or lipid glycosylation. De novo variants in the SLC35A2 gene, which encodes a UDP-galactose transporter, are responsible for CDGs with an X-linked dominant manner. Common symptoms related to SLC35A2 variants include epilepsy, psychomotor developmental delay, hypotonia, abnormal facial and skeletal features, and various magnetic resonance imaging (MRI) findings. Methods : Whole-exome sequencing was performed on the patient′s DNA, and candidate variants were confirmed by Sanger sequencing cDNA anal. was performed to assess the effect of the splice site variant using peripheral leukocytes. The X-chromosome inactivation pattern was studied using the human androgen receptor assay. Results : We identified a de novo splice site variant in SLC35A2 (NM_005660.2: c.274+1G>A) in a female patient who showed severe developmental delay, spastic paraplegia, mild cerebral atrophy, and delayed myelination on MRI, but no seizures. The variant led to an aberrant splicing resulting in an in-frame 33-bp insertion, which caused an 11-amino acid insertion in the presumptive cytoplasmic loop. X-inactivation pattern was random. Partial loss of galactose and sialic acid of the N-linked glycans of serum transferrin was observed Conclusion : This case would expand the phenotypic spectrum of SLC35A2-related disorders to delayed myelination with spasticity and no seizures.

Molecular Genetics & Genomic Medicine published new progress about Brain atrophy. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Synthetic Route of 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Deppermann, Carsten published the artcileMacrophage galactose lectin is critical for Kupffer cells to clear aged platelets, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is AMR galactose lectin hepatocyte macrophage platelet Kupffer cell interaction.

Every day, megakaryocytes produce billions of platelets that circulate for several days and eventually are cleared by the liver. The exact removal mechanism, however, remains unclear. Loss of sialic acid residues is thought to feature in the aging and clearance of platelets. Using state-of-the-art spinning disk intravital microscopy to delineate the different compartments and cells of the mouse liver, we observed rapid accumulation of desialylated platelets predominantly on Kupffer cells, with only a few on endothelial cells and none on hepatocytes. Kupffer cell depletion prevented the removal of aged platelets from circulation. Ashwell-Morell receptor (AMR) deficiency alone had little effect on platelet uptake. Macrophage galactose lectin (MGL) together with AMR mediated clearance of desialylated or cold-stored platelets by Kupffer cells. Effective clearance is critical, as mice with an aged platelet population displayed a bleeding phenotype. Our data provide evidence that the MGL of Kupffer cells plays a significant role in the removal of desialylated platelets through a collaboration with the AMR, thereby maintaining a healthy and functional platelet compartment.

Journal of Experimental Medicine published new progress about Aging, animal. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Abolghasemi, Reza published the artcileTranscriptome architecture reveals genetic networks of bolting regulation in spinach, SDS of cas: 59-23-4, the main research area is transcriptome architecture genetic network bolting regulation Spinacia; Bolting; Flowering time; RNA-Seq; Spinach.

Bolting refers to the early flowering stem production on agricultural and horticultural crops before harvesting. Indeed, bolting is an event induced by the coordinated effects of various environmental factors and endogenous genetic components, which cause a large reduction in the quality and productivity of vegetable crops like spinach. However, little is known about the signaling pathways and mol. functions involved in bolting mechanisms in spinach. The genetic information regarding the transition from vegetative growth to the reproductive stage in spinach would represent an advantage to regulate bolting time and improvement of resistant cultivars to minimize performance loss. To investigate the key genes and their genetic networks controlling spinach bolting, we performed RNA-seq anal. on early bolting accession Kashan and late-bolting accession Viroflay at both vegetative and reproductive stages and found a significant number of differentially expressed genes (DEGs) ranging from 195 to 1230 in different comparisons. These genes were mainly associated with the signaling pathways of vernalization, photoperiod/circadian clock, gibberellin, autonomous, and aging pathways. Gene ontol. anal. uncovered terms associated with carbohydrate metabolism, and detailed anal. of expression patterns for genes of Fructose-1, 6-bisphosphate aldolase, TREHALOSE-6-PHOSPHATE SYNTHASE 1, FLOWERING PROMOTING FACTOR 1, EARLY FLOWERING, GIGANTEA, and MADS-box proteins revealed their potential roles in the initiating or delaying of bolting. This study is the first report on identifying bolting and flowering-related genes based on transcriptome sequencing in spinach, which provides insight into bolting control and can be useful for mol. breeding programs and further study in the regulation of the genetic mechanisms related to bolting in other vegetable crops.

BMC Plant Biology published new progress about Carbohydrates Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (metabolism). 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Sun, Shuang published the artcileCharacterization of polysaccharide from Helicteres angustifolia L. and its immunomodulatory activities on macrophages RAW264.7, Application In Synthesis of 59-23-4, the main research area is Helicteres polysaccharide immunomodulatory agent macrophage; Helicteres angustifolia L.; Immunomodulatory activity; Macrophages; Polysaccharide.

Helicteres angustifolia L. (H. angustifolia) has been widely used as a remedy against various types of illness relating to immune response, such as inflammations and fever. In order to characterize the structure and identify the immunomodulatory activity of polysaccharide from H. angustifolia, a polysaccharide fraction (SPF3-1) was purified from H. angustifolia by using DEAE Sepharose Fast Flow and Sephacry S-400 chromatog., successively. Physicochem. anal. demonstrated that SPF3-1 is an acidic heteropolysaccharide with a mol. weight of about 13.36 kDa; in vitro immunomodulatory assay reflects that SPF3-1 could significantly (p < 0.05) enhance the proliferation of macrophages, stimulate the macrophages phagocytic capacity, as well as induce NO and immunomodulatory cytokines generation. All the results suggest that SPF3-1 from H. angustifolia possesses potent immunomodulatory activity and could be further developed as new products for medicines or functional foods. Biomedicine & Pharmacotherapy published new progress about Cell proliferation. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Application In Synthesis of 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Zhang, Liang published the artcileCombined effects of glycosylation precursors and lactate on the glycoprofile of IgG produced by CHO cells, Application In Synthesis of 59-23-4, the main research area is CHO cell monoclonal antibodies IgG; Chinese hamster ovary cells (CHO cells); Glycosylation; IgG; Monoclonal antibody; Sugars; mAbs.

The glycosylation profile of therapeutic monoclonal antibodies (mAbs) is a crucial quality parameter for industrial IgG (IgG) production Several alternative carbon sources, which function as glycosylation precursors, have been reported to impact the glycosylation pattern. Since the cells give priority to glucose uptake, the presence of this substrate can lower the effects of alternative sugars on the glycosylation. In order to get a better understanding of the influence of alternative sugars on the glycosylation and to investigate how they impact each other, combinations of mannose, fructose, galactose and fucose were fed to Chinese hamster ovary (CHO) cells in batch culture when the glucose became depleted and the lactate, accumulated in the culture, was used as carbon source. Feeding with a feed containing mannose or glucose decreased by 3-7% the percentage of high mannose glycans compared to a feed without mannose or glucose. Feeding with a feed containing galactose led to 8-20% increase of monogalactoglycans (G1) glycans and 2-6% rise of digalactoglycans (G2) glycans compared to feeding without galactose or glucose. The cells fed with fucose exhibited a significantly higher concentration of intracellular GDP-Fucose. This work indicates that a feeding strategy based on non-glucose sugars and potentially lactate, could be adopted to obtain a targeted glycosylation profile.

Journal of Biotechnology published new progress about Carbon sources, microbial. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Application In Synthesis of 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Black, Alyson P. published the artcileA Novel Mass Spectrometry Platform for Multiplexed N-Glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-Glycan Imaging, SDS of cas: 59-23-4, the main research area is mass spectrometry imaging glycoprotein biomarker antibody panel.

A new platform for N-glycoprotein anal. from serum that combines matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) workflows with antibody slide arrays is described. Antibody panel based (APB) N-glycan imaging allows for the specific capture of N-glycoproteins by antibodies on glass slides and N-glycan anal. in a protein-specific and multiplexed manner. Development of this technique has focused on characterizing two abundant and well-studied human serum glycoproteins, alpha-1-antitrypsin and IgG. Using purified standard solutions and 1 μL samples of human serum, both glycoproteins can be immunocaptured and followed by enzymic release of N-glycans. N-Glycans are detected with a MALDI FT-ICR mass spectrometer in a concentration-dependent manner while maintaining specificity of capture. Importantly, the N-glycans detected via slide-based antibody capture were identical to that of direct anal. of the spotted standards As a proof of concept, this workflow was applied to patient serum samples from individuals with liver cirrhosis to accurately detect a characteristic increase in an IgG N-glycan. This novel approach to protein-specific N-glycan anal. from an antibody panel can be further expanded to include any glycoprotein for which a validated antibody exists. Addnl., this platform can be adapted for anal. of any biofluid or biol. sample that can be analyzed by antibody arrays.

Analytical Chemistry (Washington, DC, United States) published new progress about Blood analysis. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Ji, Xiaolong published the artcileAn acidic polysaccharide from Ziziphus Jujuba cv. Muzao: Purification and structural characterization, Category: alcohols-buliding-blocks, the main research area is acidic polysaccharide Ziziphus purification structure; Characterization; Polysaccharide; Purification; Ziziphus Jujuba cv. Muzao.

A novel acidic polysaccharide fraction (PZMP2-2) was isolated from Ziziphus Jujuba cv. Muzao fruit via hot water extraction and purified by DEAE-Sepharose FF and Sephacryl S-300 column chromatog. PZMP2-2 consisted of rhamnose, arabinose, xylose, galactose, and galacturonic acid in a ratio of 1.18: 5.23: 0.22: 2.68: 2.20, with a mol. weight of 62.73 kDa. Structural features of PZMP2-2 were investigated using FT-IR, SEM, AFM, GC-MS anal. and NMR spectroscopy. Methylation and GC-MS anal. revealed that the main glycosidic bonds in PZMP2-2 comprised 1-linked Galp, 1,3-linked Araf, 1,2,4-linked Rhap, 1,3-linked Galp, 1,4-linked GalpA and 1,3,5-linked Araf. NMR anal. indicate that PZMP 2-2 has a linear backbone of (1 → 4)-linked GlcpA and (1 → 2,4)-linked Rhap residues, with branches at the O-4 position, consisting of Araf and Galp residues.

Food Chemistry published new progress about Liquid chromatography. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Category: alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts