Category: 59-23-4

Usoltseva, Roza V. published the artcileComparison of structure and in vitro anticancer activity of native and modified fucoidans from Sargassum feldmannii and S. duplicatum, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is Sargassum colon cancer cell fucoidan cytotoxicity anticancer; Anticancer activity; Fucoidan; Sargassum.

Fucoidans are valuable biol. active polysaccharides of brown algae. The aim of this study was to investigate the structure of fucoidan from Sargassum feldmannii and the anticancer effects of native and modified polysaccharides from S. feldmannii and S. duplicatum. The structure of sulfated (25.3%) galactofucan SfF2 (Fuc/Gal = 72/28 mol%) from S. feldmannii was investigated by NMR spectroscopy of desulfated derivative and mass spectrometry of fucoidan fragments labeled with 18O. SfF2 was shown to contain the main chain from 1,3-linked a-L-fucopyranose and b-D-galactopyranose residues with fucose branches at C4 and C6 of galactose residues and C2 of fucose residues. The following fragments were also identified in SfF2: Fuc-(1,4)-Fuc, Gal-(1,3)-Gal, and Gal-(1,4)-Gal. The sulfate groups occupied positions C2, C3, and C4 of fucose residues and C2, C3, C4, and C6 of galactose residues. The galactofucans from S. feldmannii, S. duplicatum, and their derivatives exhibited no cytotoxicity in vitro. The native and deacetylated fucoidans (200 microg/mL) inhibited colony formation of human colon cancer cells (DLD-1, HT-29, and HCT-116). Both desulfated fucoidans possessed weak anticancer activity.

International Journal of Biological Macromolecules published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Iwasawa, Shinya published the artcileThe prevalence of GALM mutations that cause galactosemia: A database of functionally evaluated variants, Formula: C6H12O6, the main research area is GALM mutation diagnosis galactosemia; GALM; Galactose; Galactose mutarotase; Genetics; Leloir pathway.

Galactosemia is a metabolic disorder that affects the appropriate metabolism of β-D-galactose. Deficiencies in three of the enzymes of the Leloir pathway, namely, GALT, GALK1, or GALE, are characterized as type I, II, and III galactosemia, resp. Recently, we reported a novel type of galactosemia (type IV galactosemia) due to biallelic GALM mutations. Genetic diagnosis is indispensable for diagnosing GALM deficiency because no biochem. diagnosis method has been established. Given that apparently pathogenic variants in GALM are found in public variant databases, we presumed the presence of pathogenic variants that have not been reported. In this study, we explore 67 GALM variants that are prevalent in the ExAC database, including 57 missense variants, 7 stop-gain variants, 2 frameshift variants, and 1 splice-site variant. We performed an in vitro expression assay and an enzyme activity assay. Among the 66 variants except for 1 splice-site variant, 29 produced no or faint protein expression and were judged as pathogenic variants. Furthermore, the remaining 37 variants were evaluated by enzyme activity assay. Two showed mildly reduced enzyme activity and were classified as benign. Based on our study, the estimated incidence of GALM deficiency is 1:228,411 in all populations, 1:10,388 in the African population, and 1:80,747 in the Japanese population. Our GALM mutation database is useful for the genetic diagnosis of GALM deficiency.

Molecular Genetics and Metabolism published new progress about Allele frequency. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Formula: C6H12O6.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Chen, Pei published the artcileA cold-water soluble polysaccharide isolated from Grifola frondosa induces the apoptosis of HepG2 cells through mitochondrial passway, HPLC of Formula: 59-23-4, the main research area is Grifola polysaccharide anticancer agent apoptosis signaling hepatocellular carcinoma; Cell apoptosis; Cold-water-soluble polysaccharide; Grifola frondosa polysaccharide; Mitochondrial pathway.

Grifola frondosa is a widely eaten and medicinal fungus. In this study, we extracted a cold-water-soluble polysaccharide from Grifola frondosa (cGFP) and investigated its effects on the proliferation and apoptosis of human hepatoma HepG2 cells. MTT assay showed that cGFP induced apoptosis of HepG2 cells in a dose-dependent manner. Flow cytometry anal. showed that cGFP induced apoptosis in HepG2 cells through S phase arrest. The distribution of cells at different apoptotic stages was determined by Annexin V-FITC and Propidium Iodide (PI) staining. SEM (SEM) results indicated that cGFP induced typical apoptotic morphol. features in HepG2. Mitochondrial membrane potential was reduced according to the screening of JC-1 staining. And western blot anal. of Bax, Bcl-2, cytochrome C (Cyto-c), caspase-3, and caspase-9 further demonstrated that the cGFP-induced apoptosis effect functioned through the mitochondrial pathway. Further anal. by qRT-PCR showed that Bax expression increased and Bcl-2 expression decreased. These findings suggested that cGFP could inhibit the proliferation of HepG2 cells and induce apoptosis mainly through the intrinsic activation mitochondrial pathway.

International Journal of Biological Macromolecules published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, HPLC of Formula: 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Tian, Wen-Tan published the artcileStructural characterization of an acid polysaccharide from Pinellia ternata and its induction effect on apoptosis of Hep G2 cells, SDS of cas: 59-23-4, the main research area is Pinellia acid polysaccharide cell apoptosis induction effect; Antitumor activity; Pinellia ternata polysaccharide; Structure.

In the present study, a polysaccharide fraction (PTP) was isolated and purified from the tubers of Pinellia ternata. We researched its structure and anti-tumor activity, and further studied its mol. mechanism of inducing apoptosis of Hep G2 cells. The results indicated that PTP was an acid heteropolysaccharide and the average mol. weight of PTP identified by HPGPC was 3.06 x 106 Da. Ion chromatog. (IC) determined that PTP was mainly composed of Ara:Gal:Glu:Man:GlcA:GalA in a molar ratio of 6.98:16.56:7.25:2.04:1:4.16. Combined with the results of FT-IR and NMR spectroscopy, it was found that PTP is a pyranose containing a-configuration and β-configuration, mainly consist of β-D-Gal, a-D-Glu, a-D-Ara and β-D-Man. By analyzing the results of MTT, cell cycle, Annexin V-FITC/PI double staining and cell morphol. observation, we concluded that PTP induced dose-dependent apoptosis of Hep G2 cells via S phase arrest. In addition, mitochondrial membrane potential detection and Western blot further indicated that PTP was capable of inducing apoptosis in Hep G2 cells through an endogenous mitochondria-mediated apoptotic pathway.

International Journal of Biological Macromolecules published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Li, Ying published the artcilePurification, characterization and anti-tumor activities of polysaccharides from Ecklonia kurome obtained by three different extraction methods, Formula: C6H12O6, the main research area is Ecklonia breast cancer cell polysaccharide extraction antitumor; Antitumor activity; Ecklonia kurome; Extraction methods; Polysaccharides.

To investigate and compare the effects of different extraction methods on the structure and anti-tumor activity of Ecklonia kurome polysaccharides (EP), three techniques, namely hot water extraction (HW), ultrasonic-assisted extraction (UA) and enzyme-assisted extraction (EA), were used to extract EP, and three crude EPs were purified by DEAE-cellulose and gel filtration chromatog. The significant antitumor active components in each method were screened by MTT assay and named as HW-EP5, UA-EP4 and EA-EP3, resp. The mol. weight, FT-IR assay and NMR showed that HW-EP5, UA-EP4 and EA-EP3 were pyran polysaccharides with a mol. weight of 14,466, 15,922 and 16,947 Da, resp. HW-EP5 contained the most monosaccharides and the highest content of sulfate and uronic acid. HW-EP5 had an even and smooth sheet-like appearance, while UA-EP4 and EA-EP3 exhibited irregular and rough fragments. All three polysaccharides can inhibit the migration of human breast cancer cells (MCF-7) and promote its apoptosis. All three polysaccharides promoted caspase activity during apoptosis. HW-EP5 and UA-EP4 up-regulated the expression of proapoptotic proteins Bax and p53, while EA-EP3 only up-regulated the expression of p53. These exptl. results indicate that Ecklonia kurome polysaccharides, especially HW-EP5, have great potential as a natural medicine for the treatment of breast cancer.

International Journal of Biological Macromolecules published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Formula: C6H12O6.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Dong, Xiao-dan published the artcileA novel polysaccharide from Castanea mollissima Blume: Preparation, characteristics and antitumor activities in vitro and in vivo, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is antitumor castanea polysaccharide CMP90 proliferation apoptosis cytokines immune cells; Antitumor; Castanea mollissima Blume; Characteristics; Polysaccharide.

A new water-soluble polysaccharide, CMP90, with a mol. weight of 23.9 kDa was isolated from Castanea mollissima Blume and the preliminary structural characteristics and antitumor effects of CMP90 in vitro and in vivo were investigated in the research. CMP90 consists of arabinose, galactose, glucose, xylose and mannose (molar ratio: 0.08:0.11:5.14:0.12:0.08) with α- and β-anomeric units. The results of in vitro experiments indicated that CMP90 exhibited a significant inhibitory effect on the proliferation of HL-60 cells with typical apoptotic characteristics by inducing cell cycle arrested at G1/M phase. Addnl., the results in vivo suggested CMP90 was able to inhibit the growth of S180 solid tumors via protecting immune organs, improving the levels of serum cytokines (TNF-α, IL-2 and IFN-γ), enhancing the activities of immune cells (macrophages, lymphocytes and NK cells) and inducing cell apoptosis or death. Taken together, these combined data clearly indicated that CMP90 may be used as a potential candidate agent for cancer therapy.

Carbohydrate Polymers published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Feng, Ying-ying published the artcilePolysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice, SDS of cas: 59-23-4, the main research area is Atractylodes macrocephala Koidz polysaccharide apoptosis; Antitumor activity; Atractylodes macrocephala Koidz; Characteristics; Polysaccharide.

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) was extracted by alc. sedimenting ranging from 60% to 90% and purified by ultrafiltration membrane. The high-performance gel permeation chromatog. (HPGPC), Fourier-transform IR spectroscopy (FT-IR), gas chromatog. (GC) and NMR (NMR) revealed that PAMK was a 4.1KDa neutral heteropolysaccharide composed of galactose, arabinose and glucose with a-configuration (molar ratio, 1: 1.5: 5). Results of determination of chem. components suggested that PAMK contained 96.47% of polysaccharide and little protein, nucleic acid and uronic acid. Antitumor experiments in vivo could be concluded that PAMK had a significantly cytotoxic and anti-tumor effect by blocking tumor cells in S phase. And not only that, PAMK could protect immune organ efficiently, comparing with cyclophosphamide. The research provided a potential antineoplastic drug component for tumor treatment.

International Journal of Biological Macromolecules published new progress about Antitumor agents. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Bober, Josef R. published the artcileGalactose to tagatose isomerization at moderate temperatures with high conversion and productivity, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is Lactobacillus sakei arabinose isomerase galactose tagatose.

There are many industrially-relevant enzymes that while active, are severely limited by thermodn., kinetic, or stability issues (isomerases, lyases, transglycosidases). In this work, we study Lactobacillus sakei L-arabinose isomerase (LsLAI) for D-galactose to D-tagatose isomerization-that is limited by all three reaction parameters. The enzyme demonstrates low catalytic efficiency, low thermostability at temperatures > 40°C, and equilibrium conversion < 50%. After exploring several strategies to overcome these limitations, we show that encapsulating LsLAI in gram-pos. Lactobacillus plantarum that is chem. permeabilized enables reactions at high rates, high conversions, and elevated temperatures In a batch process, this system enables ∼ 50% conversion in 4 h starting with 300 mM galactose (an average productivity of 37 mM h-1), and 85% conversion in 48 h. We suggest that such an approach may be invaluable for other enzymic processes that are similarly kinetically-, thermodynamically-, and/or stability-limited. Nature Communications published new progress about Growth, microbial. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Ge, Xinyan published the artcileProduction, structure, and bioactivity of polysaccharide isolated from Tremella fuciformis XY, Recommanded Product: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is polysaccharide Tremella fuciformis; Antioxidant activity; Fermentation optimization; Structure characterization; Tremella fuciformis polysaccharide.

Tremella fuciformis polysaccharide (TFP) is an important bioactive substance in Tremella, that contributes to its use as medicine and food. In this study, a novel fungus Tremella fuciformis XY was isolated and introduced to produce macromol. polysaccharides (TFPB). The fermentation conditions were optimized and results demonstrates that the initial pH, optimal temperature and liquid volume were 6.0, 26°C and 80 mL in a 500 mL flask, resp. The maximum yield of TFPB was 9.05 ± 0.05 g/L, which is 59.05% higher than the basic yield (5.69 ± 0.02 g/L). The TFPB, purified by DEAE column, had a mol. weight (Mw) of 1.14 × 103 kDa and consisted mainly of mannose, glucuronic acid, glucose, galactose, xylose, and rhamnose at a molar ratio of 3.5:1.2:2:1.6:1.4:3. The structure of TFPB was preliminarily investigated by methylation anal., IR spectroscopy, and NMR anal. The main linkage types were identified as 1,4-xylp, 1,4-manp, 1-xylp, 1-manp, 1,4-glcp, and 1,3,4-galp. Moreover, the antioxidant assays showed that TFPB could scavenge reactive oxygen species and hydroxyl radicals.

International Journal of Biological Macromolecules published new progress about Growth, microbial. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Recommanded Product: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Zhu, Rugang published the artcileCharacterization of polysaccharide fractions from fruit of Actinidia arguta and assessment of their antioxidant and antiglycated activities, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is polysaccharide Actinidia antioxidant AGE; Actinidia argute; Antiglycation; Antioxidation; Characterization; Polysaccharide.

A novel cell-wall polysaccharides (AAPs) were extracted from the fruits of Actinidia arguta and separated into four parts which were named water-eluted polysaccharide (WPS), salt-eluted polysaccharide (SPS)-1, SPS-2 and SPS-3. The monosaccharide composition and structural anal. showed that SPS-3 and SPS-2 were homogalacturonan (HG)-rich pectin, SPS-1 was rhamnogalacturonan (RG)-rich pectin and WPS was starch-like polysaccharides. All four kinds of polysaccharides displayed the ability to scavenge free radicals, chelate iron ion, inhibit lipid peroxidation and inhibit protein glycation, but SPS was obviously stronger than WPS. Especially SPS-3 displayed the strongest antioxidant and anti-glycated activities. In addition, the inhibitory effect of AAPs on AGEs formation is attributed to the inhibitory activity on the production of protein carbonyl group and the protective effects on the protein thiol group but not the scavenging capacity of dicarbonyl compounds, suggesting that its mechanisms of antiglycated effects may be of concern to their antioxidant activities.

Carbohydrate Polymers published new progress about Actinidia arguta. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Name: (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts