Category: 526-98-7

Lu, Xinli published the artcileDetermination of 2-keto-L-gulonic acid in fermented broth by flow injection chemiluminescence using potassium permanganate-rhodamine B system, Product Details of C6H10O7, the publication is Asian Journal of Chemistry (2013), 25(18), 10179-10181, database is CAplus.

In this paper, a flow injection-chemiluminescence (FI-CL) was presented for the determination of 2-keto-L-gulonic acid in fermented broth. This method was based on enhance effect of 2-keto-L-gulonic acid on the chemiluminescence reaction between rhodamine B and potassium permanganate in acid medium. The optimized exptl. conditions were evaluated. Under optimum conditions, calibration curve over the range of 0.2-60 mg L^-1 was obtained. The detection limit of this method was 0.04 mg L^-1. The relative standard deviation was 3.7 % for 2.0 mg L^-1 2-keto-L-gulonic acid. The method validation has been compared vs. HPLC method for determination of 2-keto-L-gulonic acid in fermented broth.

Asian Journal of Chemistry published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Product Details of C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Zhou, Jingwen published the artcileMetabolic engineering of microorganisms for vitamin C production, Computed Properties of 526-98-7, the publication is Subcellular Biochemistry (2012), 241-259, database is CAplus and MEDLINE.

A review. Vitamin C, an important organic acid, is widely used in the industries of pharmaceuticals, cosmetics, food, beverage and feed additives. Compared with the Reichstein method, biotechnol. production of vitamin C is an attractive approach due to the low cost and high product quality. In this chapter, biosynthesis of vitamin C, including one-step fermentation processes and two-step fermentation processes are discussed and compared. Furthermore, the prospects of the biotechnol. production of vitamin C are also presented.

Subcellular Biochemistry published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C17H19N3O6, Computed Properties of 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Wang, En-Xu published the artcileReorganization of a synthetic microbial consortium for one-step vitamin C fermentation, Product Details of C6H10O7, the publication is Microbial Cell Factories (2016), 21/1-21/11, database is CAplus and MEDLINE.

Background: In the industry, the conventional two-step fermentation method was used to produce 2-keto-L-gulonic acid (2-KGA), the precursor of vitamin C, by three strains, namely, Gluconobacter oxydans, Bacillus spp. and Ketogulonicigenium vulgare. Despite its high production efficiency, the long incubation period and an addnl. second sterilization process inhibit the further development. Therefore, we aimed to reorganize a synthetic consortium of G. oxydans and K. vulgare for one-step fermentation of 2-KGA and enhance the symbiotic interaction between microorganisms to perform better. Results: During the fermentation, competition for sorbose of G. oxydans arose when co-cultured with K. vulgare. In this study, the competition between the two microbes was alleviated and their mutualism was enhanced by deleting genes involved in sorbose metabolism of G. oxydans. In the engineered synthetic consortium (H₆ + Kv), the yield of 2-KGA (mol/mol) against d-sorbitol reached 89.7 % within 36 h, increased by 29.6 %. Furthermore, metabolomic anal. was used to verify the enhancement of the symbiotic relationship and to provide us potential strategies for improving the synthetic consortium. Addnl., a significant redistribution of metabolism occurred by co-culturing the K. vulgare with the engineered G. oxydans, mainly reflected in the increased TCA cycle, purine, and fatty acid metabolism Conclusions: We reorganized and optimized a synthetic consortium of G. oxydans and K. vulgare to produce 2-KGA directly from d-sorbitol. The yield of 2-KGA was comparable to that of the conventional two-step fermentation The metabolic interaction between the strains was further investigated by metabolomics, which verified the enhancement of the mutualism between the microbes and gave us a better understanding of the synthetic consortium.

Microbial Cell Factories published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C10H2F12NiO4, Product Details of C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Pothakos, Vasileios published the artcileFermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing, Product Details of C6H10O7, the publication is Chemical Engineering & Technology (2018), 41(12), 2358-2365, database is CAplus and MEDLINE.

A common focus of fermentation process optimization is the product titer. Different strategies to boost fermentation titer target whole-cell biocatalyst selection, process control, and medium composition Working at higher product concentrations reduces the water that needs to be removed in the case of aqueous systems and, therefore, lowers the cost of downstream separation and purification Different approaches to achieve higher titer in fermentation are examined Energy and water consumption data collected from different Cargill fermentation plants, i.e., ethanol, lactic acid, and 2-keto-L-gulonic acid, confirm that improvements in fermentation titer play a decisive role in downstream economics and environmental footprint.

Chemical Engineering & Technology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Product Details of C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Wang, Jingchen published the artcileOptimization of synthesis process for sodium ascorbate, Safety of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Advanced Materials Research (Durnten-Zurich, Switzerland) (2012), 550-553(Pt. 1), 10-15, database is CAplus.

With 2-keto-L-gulonic acid(2KLG) and methanol as raw materials, 98% concentrated sulfuric acid as catalyst, the Me esterification reaction is occurred. Then with sodium carbonate as a transforming agent, through a conversion reaction sodium carbonate is obtained. In this experiment, the effects of reaction time, reaction temperature and reactant ratio on conversion rate of sodium ascorbate were studied. The results showed that sodium carbonate as the reactant of lactonization reaction can effectively shorten the reaction time and improve reaction yield. By experiment under the optimum process conditions: the reaction temperature is 65 °C, reaction time is 150 min and the molar ratio of 2-keto-L-gu Me to sodium carbonate is 1:0.6, the conversion rate reaches 98 % and the effect is better than with sodium bicarbonate as transforming agent.

Advanced Materials Research (Durnten-Zurich, Switzerland) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C8H10N2O2, Safety of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Liu, Liming published the artcileGelatin enhances 2-keto-L-gulonic acid production based on Ketogulonigenium vulgare genome annotation, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Journal of Biotechnology (2011), 156(3), 182-187, database is CAplus and MEDLINE.

In the two-step fermentative production of vitamin C, its precursor 2-keto-L-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare was deficient in one or more key enzymes in the de novo biosynthesis pathways of eight different amino acids (L-histidine, L-glycine, L-lysine, L-proline, L-threonine, L-methionine, L-leucine, and L-isoleucine). Among them, L-glycine, L-proline, L-threonine, and L-isoleucine play vital roles in K. vulgare growth and 2-KLG production The addition of those amino acids increased the 2-KLG productivity by 20.4%, 17.2%, 17.2%, and 11.8%, resp. Furthermore, food grade gelatin was developed as a substitute for the amino acids to increase the cell concentration, 2-KLG productivity, and L-sorbose consumption rate by 10.2%, 23.4%, and 20.9%, resp. As a result, the fermentation period decreased to 43 h in a 7-L fermentor.

Journal of Biotechnology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Liu, Pin-jian published the artcileEstablishment and optimization of the two-dimensional gel electrophoresis for total protein in Bacillus cereus, Category: alcohols-buliding-blocks, the publication is Junshi Yixue (2012), 36(12), 942-945, 953, database is CAplus.

Objective: To establish a two-dimensional gel electrophoresis (2-DE) of proteome in Bacillus cereus and to explore how B. cereus stimulates the growth of Gluconobacter oxydans to produce 2-keto-L-gulonic acid (2-KLG) after entering the stationary phase. Methods: Different parameters, including protein preparation by different ultrasonic broken time, new type lysis, pH gradient and different sample of B. cereus protein, were used to evaluate the effect of 2-DE of proteins in B. cereus. Results and Conclusion: The clear background and reproducibility of 2-DE are established under conditions of 15 min of ultrasonic broken extraction, selection of protein cleavage I, pH 4-7, 24 cm IPG strips, 80 μg loading volume, and isoelec. focus at 60 V 15 min, and 120 V 6 h.

Junshi Yixue published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Hu, Le-Le published the artcilePredicting biological functions of compounds based on chemical-chemical interactions, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is PLoS One (2011), 6(12), e29491, database is CAplus and MEDLINE.

Given a compound, how can we effectively predict its biol. function. It is a fundamentally important problem because the information thus obtained may benefit the understanding of many basic biol. processes and provide useful clues for drug design. In this study, based on the information of chem.-chem. interactions, a novel method was developed that can be used to identify which of the following eleven metabolic pathway classes a query compound may be involved with: (1) Carbohydrate Metabolism, (2) Energy Metabolism, (3) Lipid Metabolism, (4) Nucleotide Metabolism, (5) Amino Acid Metabolism, (6) Metabolism of Other Amino Acids, (7) Glycan Biosynthesis and Metabolism, (8) Metabolism of Cofactors and Vitamins, (9) Metabolism of Terpenoids and Polyketides, (10) Biosynthesis of Other Secondary Metabolites, (11) Xenobiotics Biodegradation and Metabolism It was observed that the overall success rate obtained by the method via the 5-fold cross-validation test on a benchmark dataset consisting of 3,137 compounds was 77.97%, which is much higher than 10.45%, the corresponding success rate obtained by the random guesses. Besides, to deal with the situation that some compounds may be involved with more than one metabolic pathway class, the method presented here is featured by the capacity able to provide a series of potential metabolic pathway classes ranked according to the descending order of their likelihood for each of the query compounds concerned. Furthermore, our method was also applied to predict 5,549 compounds whose metabolic pathway classes are unknown. Interestingly, the results thus obtained are quite consistent with the deductions from the reports by other investigators. It is anticipated that, with the continuous increase of the chem.-chem. interaction data, the current method will be further enhanced in its power and accuracy, so as to become a useful complementary vehicle in annotating uncharacterized compounds for their biol. functions. A dissertation.

PLoS One published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Hao, Zhi-Qiang published the artcilePreparation of porous carbon sphere from waste sugar solution for electric double-layer capacitor, Formula: C6H10O7, the publication is Journal of Power Sources (2017), 249-258, database is CAplus.

Waste sugar solution (WSS), which contains abundant 2-keto-L-gulonic acid, is harmful to the environment if discharged directly. For value-added use of the waste resource, a novel process is developed for preparation of porous C spheres by hydrothermal carbonization (HTC) of WSS followed by KOH activation. Addnl., the possible preparation mechanism of C spheres is proposed. The effects of hydrothermal and activation parameters on the properties of the C sphere are also studied. The C sphere is applied to elec. double-layer capacitor and its electrochem. performance is studied. The C sphere obtained by HTC at 180° for 12 h with the WSS/deionized H₂O volume ratio of 2/3 possess the highest specific capacitance under identical activation conditions. The specific capacitance of the C spheres can reach 296.1 F g^-1 at a c.d. of 40 mA g^-1. Besides, excellent cycle life and good capacitance retention (89.6%) are observed at 1.5 A g^-1 after 5000 cycles. This study not only provides a facile and potential method for the WSS treatment, but also achieves the high value-added recycling of WSS for the preparation of porous C spheres with superior electrochem. properties.

Journal of Power Sources published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Formula: C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Hao, Zhi-Qiang published the artcilePreparation of porous carbon spheres from 2-keto-L-gulonic acid mother liquor by oxidation and activation for electric double-layer capacitor application, Quality Control of 526-98-7, the publication is Journal of Colloid and Interface Science (2018), 20-27, database is CAplus and MEDLINE.

A strategy is proposed for the increase of sp. surface area (SSA) of porous carbon sphere (PCS) by oxidation and activation. 2-keto-L-gulonic acid mother liquor (GAML) as a high-pollution waste has a relatively high value of reutilization. For its high value-added utilization, GAML is used as the precursor for preparation of PCS as carbon-based electrode materials for elec. double-layer capacitor. PCS is prepared by hydrothermal carbonization, carbonization and KOH activation, and Fe(NO₃)₃ 9H₂O is used as an oxidizing agent during carbonization. The as-prepared PCS has excellent porosity and high SSA of 2478 m² g^-1. Meanwhile, the pore structure of PCS can be controlled by the adjustment of carbonization parameters (carbonization temperature and the loading of Fe(NO₃)₃ 9H₂O). Besides, the SSA and specific capacitance of PCS can be increased remarkably when Fe(NO₃)₃ 9H₂O is added in carbonization. The specific capacitance of PCS can reach 303.7 F g^-1 at 40 mA g^-1. PCSs as electrode material have superior electrochem. stability. After 8000 cycles, the capacitance retention is 98.3% at 2 A g^-1. The elec. double-layer capacitance of PCS is improved when CS is carbonized with Fe(NO₃)₃ 9H₂O, and the economic and environmental benefits are achieved by the effective recycle of GAML.

Journal of Colloid and Interface Science published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Quality Control of 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts