Category: 526-98-7

Sedlacko, Erin M. published the artcileMetabolomics reveals primary response of wheat (Triticum aestivum) to irrigation with oilfield produced water, Recommanded Product: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Environmental Research, database is CAplus and MEDLINE.

The reuse of oilfield produced water (PW) for agricultural irrigation has received increased attention for utility in drought-stricken regions. It was recently demonstrated that PW irrigation can affect physiol. processes in food crops. However, metabolomic evaluations are important to further discern specific mechanisms of how PW may contribute as a plant-environmental stressor. Herein, the primary metabolic responses of wheat irrigated with PW and matching salinity controls were investigated. Non-targeted gas chromatog. mass spectrometry (GC-MS) metabolomics was combined with multivariate anal. and revealed that PW irrigation altered the primary metabolic profiles of both wheat leaf and grain. Over 600 compounds (183 annotated metabolites) were detected that varied between controls (salinity control and tap water) and PW irrigated plants. While some of these changed metabolites are related to salinity stress, over half were found to be unique to PW. The primary metabolites exhibiting changes in abundance in leaf and grain tissues were amines/amino acids, organic acids, and saccharides. Metabolite pathway anal. revealed that amino acid metabolism, sugar metabolism, and nitrogen remobilization are all impacted by PW irrigation, independent of regular plant responses to salinity stress. These data, when combined with prior physiol. studies, support a multi-faceted, physio-metabolic response of wheat to the unique stressor imposed by irrigation with PW.

Environmental Research published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C11H22N2O4, Recommanded Product: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Yang, Weichao published the artcileA plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-L-gulonic acid production, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Brazilian Journal of Microbiology (2017), 48(3), 397-402, database is CAplus and MEDLINE.

A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-L-gulonic acid (2-KLG). The screening methodol. took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining pos. mutants. Theproposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.

Brazilian Journal of Microbiology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C8H5F3N4, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Yang, W. published the artcileSpaceflight-induced enhancement of 2-keto-L-gulonic acid production by a mixed culture of Ketogulonigenium vulgare and Bacillus thuringiensis, Formula: C6H10O7, the publication is Letters in Applied Microbiology (2013), 57(1), 54-62, database is CAplus and MEDLINE.

Two bacterial strains used for industrial production of 2-keto-L-gulonic acid (2-KLG), Ketogulonigenium vulgare 2 and Bacillus thuringiensis 1514, were loaded onto the spacecraft Shenzhou VII and exposed to space conditions for 68 h in an attempt to increase their fermentation productivities of 2-KLG. An optimal combination of mutants B. thuringiensis 320 and K. vulgare 2194 (KB2194-320) was identified by systematically screening the pH and 2-KLG production of 16 000 colonies. Compared with the coculture of parent strains, the conversion rate of L-sorbose to 2-KLG by KB2194-320 in shake flask fermentation was increased significantly from 82·7% to 95·0%. Furthermore, a conversion rate of 94·5% and 2-KLG productivity of 1·88 g l^-1 h^-1 were achieved with KB2194-320 in industrial-scale fermentation (260 m³ fermentor). An observed increase in cell number of K2194 (increased by 47·8%) during the exponential phase and decrease in 2-KLG reductase activity (decreased by 46·0%) were assumed to explain the enhanced 2-KLG production The results suggested that the mutants KB2194-320 could be ideal substitutes for the currently employed strains in the 2-KLG fermentation process and demonstrated the feasibility of using spaceflight to breed high-yielding 2-KLG-producing strains for vitamin C production Significance and Impact of the Study : KB2194-320, a combination of two bacterial strains bred by spaceflight mutation, exhibited significantly improved 2-KLG productivity and hence could potentially increase the efficiency and reduce the cost of vitamin C production by the two-step fermentation process. In addition, a new pH indicator method was applied for rational screening of K2, which dramatically improved the efficiency of screening.

Letters in Applied Microbiology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C18H23N3O4S, Formula: C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gong, Xiao-li published the artcileEffects of Bacillus megaterium 2980’s spore formation on 2 KGA production of Gluconobacter oxydans, Safety of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Gongye Weishengwu (2013), 43(6), 49-53, database is CAplus.

In order to determine the relationship between the spore formation of Bacillus megaterium 2980 (B, m 2980, called companion bacterium) and Gluconobacter oxydans (G. o, called acid-producing strain) in vitamin C two-step fermentation, the effects of the culture supernatant, the cytosol and the mixed component solution of companion bacterium which cultured for 12 h (not forming spores) and 36 h (forming spores) on the growth of G. o and its synthesizing ability of 2-keto-L-gulonic acid (2-KGA) were studied through the anal. of B. m 2980 growth characteristics. The results showed that when the B. m 2980 spores did not form, the culture supernatant, the cytosol and mixed component solution had low effects on the growth of acid-producing strain and its 2-KGA production The cytosol of B. m 2980 promoted the growth and 2 KGA production of G. o greater than that of the culture supernatant. After the spore formation of B. m 2980, the culture supernatant and mixed component solution of B. m 2980 affected growth and 2-KGA production of G. o significantly.

Gongye Weishengwu published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Safety of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Lyu, Shuxia published the artcileEffect of rare earth elements on vitamin C fermentation by mixed cultures, Formula: C6H10O7, the publication is International Journal of Agriculture and Biology (2014), 16(6), 1135-1140, database is CAplus.

In recent years, the research on rare earth metals has expanded to the field of microbiol. In China, the production of Vitamin C is through mixed culture fermentation, which transforms L-sorbose to 2-keto-L-gulonic acid (2-KGA), the precursor of Vitamin C. In this study we compared the yields of 2-KGA by an acid-producing strain of Ketogulonigenium vulgare that was co-cultured with different companion bacterial strains in the presence of four light rare earth elements, lanthanum, cerium, neodymium, and samarium at selected concentrations We found that all of the tested rare earth elements had a promoting effect on 2-KGA production at concentration up to 5 mM. At or above 10 mM, the yield of 2-KGA was reduced. When cultured sep. with 5 mM of given rare earth elements, the growth of two companion bacterial strains (Bacillus megaterium 25-B and B. subtilis A9) were both significantly reduced, while that of the K. vulgare strain was enhanced.

International Journal of Agriculture and Biology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Formula: C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Xiong, Xionghua published the artcileExpression, purification and characterization of a quinoprotein L-sorbose dehydrogenase from Ketogulonicigenium vulgare Y25, Category: alcohols-buliding-blocks, the publication is African Journal of Microbiology Research (2013), 7(24), 3117-3124, database is CAplus.

It is well known that Ketogulonicigenium vulgare Y25 could effectively oxidize L-sorbose to 2-keto-L-gulonic acid (2KGA), an industrial precursor of vitamin C. There in, L-sorbose dehydrogenase is one of the key enzymes responsible for the production of 2KGA. From this organism, the coding region of sdh gene was cloned into pET22b plasmid and its transcription product was overexpressed. This procedure allowed purification of L-sorbose dehydrogenase and production of polyclonal antibodies. In Western blot assays, the antibodies gave a pos. reaction against bacteria protein extract and purified L-sorbose dehydrogenase. The mol. mass of the enzyme was 60532 Da and the N-terminal amino acid sequence was determined to be QTAIT. The Native-PAGE and resting-cell reaction assay showed that purified L-sorbose dehydrogenase could convert L-sorbose to 2KGA and PQQ was found to be indispensable for its activity as prosthetic group. The enzyme showed broad substrates specificity and the K_m value for -sorbose and 1-propanol was 21.9 mM and 0.13 mM, resp. The optimum pH of the enzyme activity was 8.0 and the optimum temperature was 35°C. The activity of the L-sorbose dehydrogenase was greatly stimulated by Ca²⁺ and strongly inhibited by Co²⁺ and Cu²⁺. The results obtained from the present study showed that a PQQ-dependent L-sorbose dehydrogenase could oxidize -sorbose into 2-keto-L-gulonic acid in vitro.

African Journal of Microbiology Research published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C11H14O4, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Lu, Shu-xia published the artcileOptimization of medium composition for two-step fermentation of vitamin C using response surface methodology, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Shipin Kexue (Beijing, China) (2013), 34(3), 202-206, database is CAplus.

2-Keto-L-gulonic acid production during the conversion process from L-sorbose to 2-keto-L-gulonic acid in the two-step fermentation of vitamin C was improved. An efficient companion strain of Bacillus subtilis A9 that facilitates the growth of Gluconobacter oxydans and the production of acids was screened from fresh milk. Based on single factor tests, the compositions of fermentation medium were optimized by response surface methodol. The results showed that three factors including L-sorbose, corn liquor and urea revealed significant impact on the production of 2-KGA. The optimal compositions included 90 g/L L-sorbose, 14.2 g/L corn liquor, 12.2 g/L urea, 4.1 g/L CaCO₃ and 0.2 g/L MgSO₄. Under the optimal medium, the yield of 2-keto-L-gulonic acid was up to 69.74 g/L, which was close to the theor. value of 70.33 g/L with the relative error of-0.59 g/L. The established regression equation for 2-KGA revealed excellent fitting. Therefore, it is credible and can be used for practical prediction.

Shipin Kexue (Beijing, China) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Xu, Tingting published the artcileResponse surface methodology to optimize medium for 2-keto-L-gulonic acid production by new strains system in two-step vitamin C fermentation, Recommanded Product: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Weishengwuxue Zazhi (2012), 32(2), 47-53, database is CAplus.

The ability of Bacillus pumilus HJ-04 to promote the production of vitamin C (VC) precursor, 2-keto-L-gulonic acid (2-KGA), by 2-KGA-producing strain is stronger than industrial Bacillus megaterium B2980 as associated bacteria in second step of two-step VC fermentation Six factors that affected the 2-KGA production by new strains system were analyzed and optimized adopting single factor test, Plackett-Burman (PB) design, and Box-Behnken design. The results indicated that L-sorbose, urea, and corn syrup were the noticeably influencing factors. The optimum acid-producing condition was 94.95 g/L of L-sorbose, 11.99 g/L of urea, 14.13 g/L of corn syrup. The acid production increased by 12.31 mg/mL, and the production period shortened by 6 h after the optimization.

Weishengwuxue Zazhi published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C20H23N3O2S, Recommanded Product: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Yang, Yu published the artcileOptimization of medium composition for two-step fermentation of vitamin C based on artificial neural network-genetic algorithm techniques, Computed Properties of 526-98-7, the publication is Biotechnology & Biotechnological Equipment (2015), 29(6), 1128-1134, database is CAplus.

The production of 2-keto-L-gulonic acid (2-KGA) during the conversion from L-sorbose to 2-KGA in the two-step fermentation of vitamin C can be improved by using an efficient companion strain Bacillus subtilis A9 to facilitate the growth of Ketogulonicigenium vulgare and the production of 2-KGA. Two optimization models, namely response surface methodol. (RSM) and artificial neural network (ANN), were built to optimize the medium components for mixed-culture fermentation of 2-KGA. The root mean square error, R2 and the standard error of prediction given by the ANN model were 0.13%, 0.99% and 0.21%, resp., while the RSM model gave 1.89%, 0.84% and 2.9%, resp. This indicated that the fitness and the prediction accuracy of the ANN model were higher than those of the RSM model. Furthermore, using genetic algorithm (GA), the input space of the ANN model was optimized, predicting that the maximum 2-KGA production of 72.54 g·L-1 would be obtained at the GA-optimized concentrations of the medium components (L-sorbose, 92.5 g·L-1; urea, 10.2 g·L-1; corn steep liquor, 16 g·L-1; CaCO3, 3.96 g·L-1; MgSO4, 0.28 g·L-1). The 2-KGA production exptl. obtained using the ANN-GA-designed medium was 71.21 ± 1.53 g·L-1, which was in good agreement with the predicted value. The same optimization process may be used to improve the production during bacterial mixed-cultures fermentation by changing the fermentation parameters.

Biotechnology & Biotechnological Equipment published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H8N2, Computed Properties of 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Jirasek, Fabian published the artcileSolid-Liquid Equilibrium in the System 2-Keto-L-Gulonic Acid + L-Ascorbic Acid + Water, SDS of cas: 526-98-7, the publication is Chemical Engineering & Technology (2018), 41(12), 2306-2311, database is CAplus and MEDLINE.

The solid-liquid equilibrium (SLE) in the ternary system 2-keto-L-gulonic acid (HKGA) + L-ascorbic acid (vitamin C) + water was investigated exptl. at temperatures between 276 K and 308 K at ambient pressure, i.e., under conditions that are of particular interest for industrial applications. Phase diagrams with one eutonic point were obtained for all temperatures The dissociation constant and the solubility constant of vitamin C were determined as a function of temperature Based on an extended version of the Debye-Hueckel theory, a physicochem. model was developed that describes the SLE in the ternary system. The agreement between exptl. data and results from the model is excellent.

Chemical Engineering & Technology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, SDS of cas: 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts