Category: 35564-86-4

Tran, Johan M.; Farrell, Melanie A.; Fanestil, Darrell D. published the artcile< Effect of ions on binding of the thiazide-type diuretic metolazone to kidney membrane>, Synthetic Route of 35564-86-4, the main research area is kidney metolazone sodium chloride transport.

The effect of a number of ions on the binding of the thiazide-type diuretic metolazone (MTZ) to rat renal cortical membranes was studied to elucidate the mechanism of NaCl transport in the kidney distal tubule. Among the cations tested, Na+ stimulated the binding up to 2.4-fold over control. The effective concentration of Na+ that produced half-maximal stimulation was 2-17 mM. Li+, K+, NH4+, Rb+, and Cs+ produced little stimulation of binding of MTZ. Several anions including Cl- inhibited binding. The inhibition of binding of MTZ by Cl- was enhanced by Na+ and Li+. Scatchard anal. revealed that 50 mM Na+ increased the affinity for binding of MTZ from a Kd = 3.56 nM to Kd = 1.32 nM. Chloride, in the presence of 50 mM Na+, competitively inhibited binding of MTZ by suppressing the affinity to Kd = 9.27 nM without changing the maximal number of binding sites (0.733 pmol/mg). A mechanism for the MTZ-sensitive NaCl transport is proposed, in which the transporter protein possesses a binding site for Na+ and a binding site for Cl-, which is also the binding site for MTZ. Na+ binds to its site and increases the affinity for Cl-/MTZ. The binding of Cl- to the transporter enables the import of Na+ and Cl- across the tubule membrane. MTZ, however, when present competes with Cl- for the binding site on the transporter and prevents the transport of Na+ and Cl-.

American Journal of Physiology published new progress about Biological cotransport. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Synthetic Route of 35564-86-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Kraudelt, H.; Schilde, U.; Uhlemann, E. published the artcile< Crystal structures of 1-methylamino-D-1-deoxyglucitol, C7H17NO5 and 1-methylamino-D-1-deoxyglucitol hydrochloride, C7H18ClNO5>, Recommanded Product: N-Methyl-D-glucamine Hydrochloride, the main research area is mol structure methylaminodeoxyglucitol hydrochloride.

The 1st title compound is orthorhombic, space group P212121, a 4.615(1), b 10.240(2), c 19.810(9) Å, Z = 4, R = 0.040, Rw = 0.094 for 1787 reflections. The 2nd title compound is monoclinic, space group P21, a 7.401(2), b 8.681(3), c 8.789(4) Å, β 105.30(2)°, Z = 2, R = 0.026, Rw = 0.072 for 1592 reflections. At. coordinates are given. The H bonding in both mols. is discussed and some values given.

Zeitschrift fuer Kristallographie – New Crystal Structures published new progress about Crystal structure. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Recommanded Product: N-Methyl-D-glucamine Hydrochloride.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Knoefel, Peter K.; Kraft, Robert P. Jr.; Knight, Robert D.; Moore, Susan K. published the artcile< Sodium versus mmeglumine diatrizoate in excretory urography>, Formula: C7H18ClNO5, the main research area is urinary excretion sodium meglumine diatrizoate; electrolyte excretion urine diatrizoate.

The i.v. injection of mannitol [69-65-8], Na diatrizoate (I) [737-31-5] meglumine diatrizoate (II) [131-49-7] or meglumine hydrochloride [35564-86-4] produced diuresis approaching a steady-state in anesthetized dogs. During this period the apparent volumes of distribution of meglumine and diatrizoate were similar and were greater than that of insulin. The plasma concentration, renal clearance, and the rate of urinary excretion were the same for meglumine and diatrizoate. Excretion of urine and urinary electrolytes was greater after II than I or meglumine-HCl. Urinary concentration of diatrizoate was greater after I than II. This difference appears to result from the influence of the 2 impermeant ions on the excretion of other ions. Single-dose experiments revealed no such difference between I and II due to errors arising from collection of urine from the bladder.

Investigative Radiology published new progress about Urinary system. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Formula: C7H18ClNO5.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Carr, Georgina; Haslam, Iain S.; Simmons, Nicholas L. published the artcile< Voltage Dependence of Transepithelial Guanidine Permeation Across Caco-2 Epithelia Allows Determination of the Paracellular Flux Component>, COA of Formula: C7H18ClNO5, the main research area is guanidine organic cation paracellular permeation intestine Caco2 cell.

The aim of this study was to investigate transepithelial ionic permeation via the paracellular pathway of human Caco-2 epithelial monolayers and its contribution to absorption of the base guanidine. Confluent monolayers of Caco-2 epithelial cells were mounted in Ussing chambers and the transepithelial conductance and elec. p.d. determined after NaCl dilution or medium Na substitution (bi-ionic conditions). Guanidine absorption (Ja-b) was measured ± transepithelial potential gradients using bi-ionic p.d.’s. Basal NaCl replacement with mannitol gives a transepithelial dilution p.d. of 28.0 ± 3.1 mV basal solution electropos. (PCl/PNa = 0.34). Bi-ionic p.d.’s (basal replacements) indicate a cation selectivity of NH4+ > K+∼Cs+ > Na+ > Li+ > tetraethylammonium+ > N-methyl-d-glucamine+∼choline+. Transepithelial conductances show good correspondence with bi-ionic potential data. Guanidine Ja-b was markedly sensitive to imposed transepithelial p.d. The ratio of guanidine to mannitol permeability (measured simultaneously) increased from 3.6 in the absence of an imposed p.d. to 13.8 (basolateral neg. p.d.). Hydrated monovalent ions preferentially permeate the paracellular pathway (Eisenman sequence 2 or 3). Guanidine may access the paracellular pathway because absorptive flux is sensitive to the transepithelial p.d. An alternative method to assess paracellular-mediated flux of charged organic mols. is suggested.

Pharmaceutical Research published new progress about Biological permeation (transepithelial ionic). 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, COA of Formula: C7H18ClNO5.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Lindberg, B.; Asplund, L.; Fellner-Feldegg, H.; Kelfve, P.; Siegbahn, H.; Siegbahn, K. published the artcile< ESCA applied to liquids. ESCA spectra from molecular ions in solution>, Quality Control of 35564-86-4, the main research area is ESCA spectra pyridylsulfamoylphenylazosalicylate; salicylate pyridylsulfamoylphenylazo ESCA.

The application of ESCA to the N-methylglucamine salt of 5-[p-(2-pyridylsulfamoyl)phenylazo]salicylic acid is described. By exchange of one of the ions in the solution the ESCA spectra of the individual ionic components are deduced. Broadening of N lines in the spectrum indicates different chem. forms in solution

Chemical Physics Letters published new progress about Photoelectron spectra. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Quality Control of 35564-86-4.

Referemce:
Alcohol – Wikipedia,
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Chromy, V.; Kulhanek, V.; Fischer, J. published the artcile< D-(-)-N-methylglucamine buffer for pH 8.5 to 10.5>, HPLC of Formula: 35564-86-4, the main research area is methylglucamine buffer; amine buffer methylglucamine; pH buffer methylglucamine.

A new amine buffer for the pH range 8.5-10.5, based on D-(-)-N-methylglucamine (I) and its hydrochloride, is described. Important phys. and chem. constants characterizing this buffer system are: thermodn. pKa = 9.52 (25°); buffer capacity = 0.055; dilution value pH0.5 = -0.02; temperature coefficient dpKa/dT = -0.023; the heat of ionization ΔH° = 36.93 kJ/mol. I, which is available in highly purified form, is highly soluble in water. Thus, either buffer solutions or stable solid mixtures when combined with D-(-)-N-methylglucaminium chloride can be prepared I buffers are suitable for both biochem. and pH control.

Clinical Chemistry (Washington, DC, United States) published new progress about Buffers. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, HPLC of Formula: 35564-86-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Li, Xiang; Shen, Bing; Yao, Xiao-Qiang; Yang, Dan published the artcile< A Small Synthetic Molecule Forms Chloride Channels to Mediate Chloride Transport across Cell Membranes>, Quality Control of 35564-86-4, the main research area is chloride channel plasma membrane permeability bilayer.

Synthetic ion channels that mimic the functions of natural ion channels are of great interest in chem., biochem., biol., and materials science. In this paper, we present a novel small synthetic mol. that self-assembles to form chloride channels in lipid bilayers. This compound is the smallest mol. known to form potent synthetic chloride ion channels. It can also partition into plasma membranes of living cells and therein increase anion permeability. This compound has the potential to become a novel lead compound for the treatment of human diseases associated with Cl- channel dysfunctions.

Journal of the American Chemical Society published new progress about Bilayer biological membrane. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Quality Control of 35564-86-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Kesinger, Evan; Liu, Jianing; Jensen, Aaron; Chia, Catherine P.; Demers, Andrew; Moriyama, Hideaki published the artcile< Influenza D virus M2 protein exhibits ion channel activity in Xenopus laevis oocytes>, Recommanded Product: N-Methyl-D-glucamine Hydrochloride, the main research area is Xenopus oocyte influenza D virus M2 protein ion channel.

A new type of influenza virus, known as type D, has recently been identified in cattle and pigs. Influenza D virus infection in cattle is typically asymptomatic; however, its infection in swine can result in clin. disease. Swine can also be infected with all other types of influenza viruses, namely A, B, and C. Consequently, swine can serve as a “”mixing vessel”” for highly pathogenic influenza viruses, including those with zoonotic potential. Currently, the only antiviral drug available targets influenza M2 protein ion channel is not completely effective. Thus, it is necessary to develop an M2 ion channel blocker capable of suppressing the induction of resistance to the genetic shift. To provide a basis for developing novel ion channel-blocking compounds, we investigated the properties of influenza D virus M2 protein (DM2) as a drug target. To test the ion channel activity of DM2, the DNA corresponding to DM2 with cMyc-tag conjugated to its carboxyl end was cloned into the shuttle vector pNCB1. The mRNA of the DM2-cMyc gene was synthesized and injected into Xenopus oocytes. The translation products of DM2-cMyc mRNA were confirmed by immunofluorescence and mass spectrometry analyses. The DM2-cMyc mRNA-injected oocytes were subjected to the two-electrode voltage-clamp (TEVC) method, and the induced inward current was observed The midpoint (Vmid) values in Boltzmann modeling for oocytes injected with DM2-cMyc RNA or a buffer were -152 and -200 mV, resp. Assuming the same expression level in the Xenopus oocytes, DM2 without tag and influenza C virus M2 protein (CM2) were subjected to the TEVC method. DM2 exhibited ion channel activity under the condition that CM2 ion channel activity was reproduced. The gating voltages represented by Vmid for CM2 and DM2 were -141 and -146 mV, resp. The reversal potentials observed in ND96 for CM2 and DM2 were -21 and -22 mV, resp. Compared with intact DM2, DM2 variants with mutation in the YxxxK motif, namely Y72A and K76A DM2, showed lower Vmid values while showing no change in reversal potential. The M2 protein from newly isolated influenza D virus showed ion channel activity similar to that of CM2. The gating voltage was shown to be affected by the YxxxK motif and by the hydrophobicity and bulkiness of the carboxyl end of the mol.

PLoS One published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Recommanded Product: N-Methyl-D-glucamine Hydrochloride.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Speck, Ulrich; Siefert, Hans Martin; Klink, Gunter published the artcile< Contrast media and pain in peripheral arteriography>, Application of C7H18ClNO5, the main research area is contrast media pain arteriog.

Pain caused by x-ray contrast media in peripheral arteriog. was assessed by behavioral changes of nonanesthetized, unrestrained rats. All ionic monomeric contrast media caused severe pain in a concentration of 300 mg I/mL. Dilution of contrast media markedly reduced pain. Solutions containing Na salts were considerably more painful than solutions with meglumine salts. The i.v. cholangiog. agent meglumine iodipamide [3521-84-4] did not cause vascular pain in a concentration of 300 mg I/mL, although its systemic toxicity was high. Pain in arteriog. was attributable primarily to the high osmotic pressure of contrast media solutions rather than to their chemotoxicity.

Investigative Radiology published new progress about Artery. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, Application of C7H18ClNO5.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Pesci, Pierantonio published the artcile< Involvement of chloride in the increase in proline induced by ABA and stimulated by potassium chloride in barley leaf segments>, SDS of cas: 35564-86-4, the main research area is ABA induction proline barley chloride.

Stimulation by NaCl or KCl of the ABA-induced increase in proline was synergistically enhanced by CaCl2 or MgCl2 as well as by 1,3-bis[tris(hydroxymethyl)methylamino]propane chloride (BTP-Cl), N-methyl-D-glucamine chloride (NMG-Cl), or 2-amino-2-hydroxymethyl-1,3-propandiol chloride (TRIS-Cl). This enhancing effect did not depend on the osmolarity and occurred when Cl- was higher than K+ in the incubation medium, but not vice versa. When CaCl2 or MgCl2 or NMG-Cl were added, the higher the Cl-:K+ ratio in the external solution the higher was the increase in proline. When the excess of Cl- to K+ was obtained with BTP-Cl the highest enhancing effect resulted with a Cl-:K+ ratio of 3:1 while, at a 5:1 ratio, the KCl stimulation was completely suppressed. The inhibiting effect of proline accumulation by NH4+ and 4,4′-diisothiocyano-2,2′-disulfonicacid stilbene was reversed to varying degrees depending on the magnitude of the excess of Cl- on K+ concentration in the medium. Also, the inhibition of proline accumulation obtained by tetraethylammonium chloride, monensin, and D-mannose was similarly reverted. Thus, Cl- elicits an increase in ABA-induced proline which requires the simultaneous presence of K+ (or Na+).

Plant Physiology published new progress about Barley. 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, SDS of cas: 35564-86-4.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts