Category: 32462-30-9

Hu, Ping; Zhao, Fangzhou; Wang, Jing; Zhu, Weiyun published an article in 2021, the title of the article was Metabolomic profiling reveals the effects of early-life lactoferrin intervention on protein synthesis, energy production and antioxidative capacity in the liver of suckling piglets.Synthetic Route of 32462-30-9 And the article contains the following content:

This study aimed to determine the effects of an early-life lactoferrin (LF) intervention on liver metabolism in suckling piglets. Sixty newborn piglets with an average initial body weight (BW) of 1.51 ± 0.05 kg were assigned to a control (CON) group and an LF group. At age 1 to 7 days, the piglets in the LF group were orally administered LF solution (0.5 g per kg BW daily), whereas the piglets in the CON group were orally administered the same dose of physiol. saline. Plasma, jejunum and liver samples were collected on days 8 and 21. The LF piglets showed a decreased plasma urea nitrogen level on day 8 and an increased plasma albumin level on day 21. Pathway anal. of the metabolomic profiles showed that the LF treatment affected amino acid metabolism in the liver. In addition, the LF treatment upregulated the gene expression levels of proteolytic enzymes and amino acid transporters (APA, APN, EAAC1, Pept1, CAT1, B0AT1 and ASCT2) in the jejunum, and it enhanced the phosphorylation levels of mTOR and p70S6K in the liver. The LF treatment also upregulated the expression of a β-oxidation-related gene (CPT1) and affected the tricarboxylic acid cycle in the liver on day 21. Furthermore, the LF piglets showed a decreased level of malondialdehyde and increased levels of GSH, GSH-Px and GCLC in the liver mitochondria. Overall, the early-life LF intervention affected the protein synthesis, energy production and antioxidative capacity in the liver of the neonatal piglets. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Synthetic Route of 32462-30-9

The Article related to liver lactoferrin protein energy antioxidative metabolomics, Animal Nutrition: Proteins and Nonprotein-Nitrogen Substances and other aspects.Synthetic Route of 32462-30-9

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Gfeller, David; Michielin, Olivier; Zoete, Vincent published an article in 2012, the title of the article was Expanding molecular modeling and design tools to non-natural sidechains.Safety of H-Phg(4-OH)-OH And the article contains the following content:

Protein-protein interactions encode the wiring diagram of cellular signaling pathways and their deregulations underlie a variety of diseases, such as cancer. Inhibiting protein-protein interactions with peptide derivatives is a promising way to develop new biol. and therapeutic tools. Here, the authors develop a general framework to computationally handle hundreds of non-natural amino acid sidechains and predict the effect of inserting them into peptides or proteins. The authors first generate all structural files (pdb and mol2), as well as parameters and topologies for standard mol. mechanics software (CHARMM and Gromacs). Accurate predictions of rotamer probabilities are provided using a novel combined knowledge and physics based strategy. Non-natural sidechains are useful to increase peptide ligand binding affinity. The authors’ results obtained on non-natural mutants of a BCL9 peptide targeting beta-catenin show very good correlation between predicted and exptl. binding free-energies, indicating that such predictions can be used to design new inhibitors. Data generated as well as PyMOL and UCSF Chimera plug-ins for user-friendly visualization of non-natural sidechains, are all available at http://www.swisssidechain.ch. The authors’ results enable researchers to rapidly and efficiently work with hundreds of non-natural sidechains. © 2012 Wiley Periodicals, Inc. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Safety of H-Phg(4-OH)-OH

The Article related to mol model design nonnatural side chain protein peptide interaction, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Safety of H-Phg(4-OH)-OH

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On March 1, 2020, Petrov, Alexander P.; Sherman, Lindy M.; Camden, Jon P.; Dovichi, Norman J. published an article.HPLC of Formula: 32462-30-9 The title of the article was Database of free solution mobilities for 276 metabolites. And the article contained the following:

Although databases are available that provide mass spectra and chromatog. retention information for small-mol. metabolites, no publicly available database provides electrophoretic mobility for common metabolites. As a result, most compounds found in electrophoretic-based metabolic studies are unidentified and simply annotated as “features”. To begin to address this issue, the authors analyzed 460 metabolites from a com. library using capillary zone electrophoresis coupled with electrospray mass spectrometry. To speed anal., a sequential injection method was used wherein six compounds were analyzed per run. An uncoated fused silica capillary was used for the anal. at 20° with a 0.5% (volume/volume) formic acid and 5% (volume/volume) methanol background electrolyte. A Prince autosampler was used for sample injection and the capillary was coupled to an ion trap mass spectrometer using an electrokinetically-pumped nanospray interface. The authors generated mobility values for 276 metabolites from the library (60% success rate) with an average standard deviation of 0.01 × 10-8 m2V-1s-1. As expected, cationic and anionic compounds were well resolved from neutral compounds Neutral compounds co-migrated with electroosmotic flow. Most of the compounds that were not detected were neutral and presumably suffered from adsorption to the capillary wall or poor ionization efficiency. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).HPLC of Formula: 32462-30-9

The Article related to metabolite solution electrophoretic mobility database, capillary electrophoresis, metabolite database, sequential injection, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.HPLC of Formula: 32462-30-9

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On December 31, 2020, Ma, Yibao; Wang, Wei; Devarakonda, Teja; Zhou, Huiping; Wang, Xiang-Yang; Salloum, Fadi N.; Spiegel, Sarah; Fang, Xianjun published an article.SDS of cas: 32462-30-9 The title of the article was Functional analysis of molecular and pharmacological modulators of mitochondrial fatty acid oxidation. And the article contained the following:

Abstract: Fatty acid oxidation (FAO) is a key bioenergetic pathway often dysregulated in diseases. The current knowledge on FAO regulators in mammalian cells is limited and sometimes controversial. Previous FAO analyses involve nonphysiol. culture conditions or lack adequate quantification. We herein described a convenient and quant. assay to monitor dynamic FAO activities of mammalian cells in physiol. relevant settings. The method enabled us to assess various mol. and pharmacol. modulators of the FAO pathway in established cell lines, primary cells and mice. Surprisingly, many previously proposed FAO inhibitors such as ranolazine and trimetazidine lacked FAO-interfering activity. In comparison, etomoxir at low micromolar concentrations was sufficient to saturate its target proteins and to block cellular FAO function. Oxfenicine, on the other hand, acted as a partial inhibitor of FAO. As another class of FAO inhibitors that transcriptionally repress FAO genes, antagonists of peroxisome proliferator-activated receptors (PPARs), particularly that of PPARa, significantly decreased cellular FAO activity. Our assay also had sufficient sensitivity to monitor upregulation of FAO in response to environmental glucose depletion and other energy-demanding cues. Altogether this study provided a reliable FAO assay and a clear picture of biol. properties of potential FAO modulators in the mammalian system. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).SDS of cas: 32462-30-9

The Article related to monocytic lymphoma breast carcinoma fatty acid oxidation mitochondria, Pharmacology: Effects Of Neoplasm Inhibitors and Cytotoxic Agents and other aspects.SDS of cas: 32462-30-9

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Dufour, Jeremy; Neuville, Luc; Zhu, Jieping published an article in 2010, the title of the article was Intramolecular Suzuki-Miyaura reaction for the total synthesis of signal peptidase inhibitors, arylomycins A2 and B2.Name: H-Phg(4-OH)-OH And the article contains the following content:

Development of the total syntheses of arylomycins A1 and B2 is detailed. Key features of our approach include (1) formation of 14-membered meta,meta-cyclophane by an intramol. Suzuki-Miyaura reaction; (2) incorporation of N-Me-4-hydroxyphenylglycine into the cyclization precursor, which avoids the late-stage low-yielding N-methylation step; (3) segment coupling of a fully elaborated peptide side chain to the macrocycle, which makes the synthesis highly convergent. Overall, arylomycin A2 was obtained in 13 steps from L-Tyr for the longest linear sequence, in 13 % overall yield. Arylomycin B2 was synthesized in 10 steps from L-3-nitro-Tyr, in 10 % overall yield. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Name: H-Phg(4-OH)-OH

The Article related to cyclic peptide arylomycin total synthesis signal peptidase inhibitor rotamer, suzuki miyaura coupling methylhydroxyphenylglycine cyclization peptide coupling, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Name: H-Phg(4-OH)-OH

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On November 15, 2019, Wong, Nicholas; Petronijevic, Filip; Hong, Allen Y.; Linghu, Xin; Kelly, Sean M.; Hou, Haiyun; Cravillion, Theresa; Lim, Ngiap-Kie; Robinson, Sarah J.; Han, Chong; Molinaro, Carmela; Sowell, C. Gregory; Gosselin, Francis published an article.HPLC of Formula: 32462-30-9 The title of the article was Stereocontrolled synthesis of arylomycin-based gram-negative antibiotic GDC-5338. And the article contained the following:

We report herein an efficient, stereocontrolled, and chromatog.-free synthesis of the novel broad spectrum antibiotic GDC-5338. The route features the construction of a functionalized tripeptide backbone, a high-yielding macrocyclization via a Pd-catalyzed Suzuki-Miyaura reaction, and the late-stage elaboration of key amide bonds with minimal stereochem. erosion. Through extensive reaction development and anal. understanding, these key advancements allowed the preparation of GDC-5338 in 17 steps, 15% overall yield, >99 A % HPLC, and >99:1 dr. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).HPLC of Formula: 32462-30-9

The Article related to peptide antibiotic arylomycin based gdc5338 stereocontrolled synthesis crystal structure, tripeptide macrocyclization suzuki miyaura reaction palladium catalyst, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.HPLC of Formula: 32462-30-9

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On October 8, 2015, Weigel, Lena F.; Nitsche, Christoph; Graf, Dominik; Bartenschlager, Ralf; Klein, Christian D. published an article.Safety of H-Phg(4-OH)-OH The title of the article was Phenylalanine and Phenylglycine Analogues as Arginine Mimetics in Dengue Protease Inhibitors. And the article contained the following:

Dengue virus is an increasingly global pathogen. One of the promising targets for antiviral drug discovery against dengue and related flaviviruses such as West Nile virus is the viral serine protease NS2B-NS3. We here report the synthesis and in vitro characterization of potent peptidic inhibitors of dengue virus protease that incorporate phenylalanine and phenylglycine derivatives as arginine-mimicking groups with modulated basicity. The most promising compounds were (4-amidino)-L-phenylalanine-containing inhibitors, which reached nanomolar affinities against dengue virus protease. The type and position of the substituents on the phenylglycine and phenylalanine side chains has a significant effect on the inhibitory activity against dengue virus protease and selectivity against other proteases. In addition, the non-natural, basic amino acids described here may have relevance for the development of other peptidic and peptidomimetic drugs such as inhibitors of the blood clotting cascade. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Safety of H-Phg(4-OH)-OH

The Article related to phenylalanine phenylglycine based arginine mimetic tripeptide preparation antiviral, dengue protease inhibitor tripeptide arginine mimetic, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Safety of H-Phg(4-OH)-OH

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On November 1, 2013, Gaudelli, Nicole M.; Townsend, Craig A. published an article.Synthetic Route of 32462-30-9 The title of the article was Stereocontrolled Syntheses of Peptide Thioesters Containing Modified Seryl Residues as Probes of Antibiotic Biosynthesis [Erratum to document cited in CA159:134091]. And the article contained the following:

The Supporting Information contained incorrectly placed NMR spectra for six figures; the corrected files are now included online. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Synthetic Route of 32462-30-9

The Article related to erratum serine phosphoserine beta lactam peptide thioester preparation, nocardicin epimerization erratum, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Synthetic Route of 32462-30-9

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On July 5, 2013, Gaudelli, Nicole M.; Townsend, Craig A. published an article.Safety of H-Phg(4-OH)-OH The title of the article was Stereocontrolled Syntheses of Peptide Thioesters Containing Modified Seryl Residues as Probes of Antibiotic Biosynthesis. And the article contained the following:

Methods have been developed to synthesize tri- and pentapeptide thioesters containing one or more p-(hydroxyphenyl)glycine (pHPG) residues and L-serine, some where the latter is O-phosphorylated, O-acetylated, or exists as a β-lactam. Selection of orthogonal protection strategies and development of conditions to achieve seryl O-phosphorylation without β-elimination and to maintain stereochem. control, especially simultaneously at exceptionally base-labile pHPG α-carbons, are described. Intramol. closure of a seryl peptide to a β-lactam-containing peptide and the syntheses of corresponding thioester analogs are also reported. Modification of classical Mitsunobu conditions is described in the synthesis of the β-lactam-containing products, and in a broadly useful observation, it was found that simple exclusion of light from the P(OEt)3-mediated Mitsunobu ring closure afforded yields of >95%, presumably owing to reduced photodegradation of the azodicarboxylate used. These sensitive potential substrates and products will be used in mechanistic studies of the two nonribosomal peptide synthetases NocA and NocB that lie at the heart of nocardicin biosynthesis, a family of monocyclic β-lactam antibiotics. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Safety of H-Phg(4-OH)-OH

The Article related to serine phosphoserine beta lactam peptide thioester preparation nocardicin epimerization, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Safety of H-Phg(4-OH)-OH

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On December 10, 2015, Behnam, Mira A. M.; Graf, Dominik; Bartenschlager, Ralf; Zlotos, Darius P.; Klein, Christian D. published an article.Name: H-Phg(4-OH)-OH The title of the article was Discovery of Nanomolar Dengue and West Nile Virus Protease Inhibitors Containing a 4-Benzyloxyphenylglycine Residue. And the article contained the following:

The dengue virus (DENV) and West Nile Virus (WNV) NS2B-NS3 proteases are attractive targets for the development of dual-acting therapeutics against these arboviral pathogens. We present the synthesis and extensive biol. evaluation of inhibitors that contain benzyl ethers of 4-hydroxyphenylglycine as non-natural peptidic building blocks synthesized via a copper-complex intermediate. A three-step optimization strategy, beginning with fragment growth of the C-terminal 4-hydroxyphenylglycine to the benzyloxy ether, followed by C- and N-terminal optimization, and finally fragment merging generated compounds with in vitro affinities in the low nanomolar range. The most promising derivative reached Ki values of 12 nM at the DENV-2 and 39 nM at the WNV proteases. Several of the newly discovered protease inhibitors yielded a significant reduction of dengue and West Nile virus titers in cell-based assays of virus replication, with an EC50 value of 3.4 μM at DENV-2 and 15.5 μM at WNV for the most active analog. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Name: H-Phg(4-OH)-OH

The Article related to hydroxyphenylglycine peptide preparation protease inhibitor dengue west nile virus, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Name: H-Phg(4-OH)-OH

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