SDS of cas: 110-03-2《Vitrigel-Eye Irritancy Test Method Using HCE-T Cells》 was published in 2013. The authors were Yamaguchi, Hiroyuki;Kojima, Hajime;Takezawa, Toshiaki, and the article was included in《Toxicological Sciences》. The author mentioned the following in the article:
The authors previously reported that the time-dependent relative changes of transepithelial elec. resistance (TEER) after exposing four different chems. to a human corneal epithelium (HCE) model were well correlated to the potential of ocular irritancy. Meanwhile, the authors recently developed a collagen vitrigel membrane (CVM) chamber possessing a scaffold composed of high-d. collagen fibrils equivalent to connective tissues in vivo as a three-dimensional culture tool. The CVM chamber is useful for biomedical assays and immunohistol. using cryosections that are inappropriate to be performed using the conventional Millicell chamber with a polyethylene terephthalate membrane. In this study, the authors aimed to develop a new eye irritancy test (EIT) method called “Vitrigel-EIT method” that can facilitate to briefly and accurately estimate the widespread irritancy of test chems. by applying the TEER assay system to a HCE model fabricated in the CVM chamber. HCE-T cells (a HCE-derived cell strain) were cultured in the CVM chamber for 6 days, and consequently, the Vitrigel-HCE model possessing the following characteristics of HCE in vivo was formed: six cell layers with specific protein expressions and their barrier function. Time-dependent profiles of TEER values after exposing 30 test chems. to the HCE model were converted into the scores of three indexes (time lag, intensity, and plateau level), and each chem. was successfully classified into irritant or nonirritant category by utilizing the criteria for the indexes, resulting in the excellent correlation with Globally Harmonized System of Classification and Labeling of Chems. (GHS) classification (sensitivity: 100%, specificity: 75%, accuracy: 90%). These data suggest that the widespread eye irritancy of chems. can be predicted without false negatives by the Vitrigel-EIT method. Interestingly, the disruption of tight junctions was immunohistol. observed after exposing not only irritants but also three compounds classified as nonirritant by GHS but found pos. in the authors’ Vitrigel-EIT method confirming a possible mild irritant property. The experimental procedure involved many compounds, such as 2,5-Dimethyl-2,5-hexanediol (cas: 110-03-2) .
2,5-Dimethyl-2,5-hexanediol(cas:110-03-2) on heteropoly acid catalyzed dehydration yields cyclic ethers via stereospecific intramolecular SN2 mechanism. It reacts with nitriles in concentrated sulfuric acid to yield Δ1-pyrrolines.SDS of cas: 110-03-2
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