Name: 2,2-Methylenebis(6-tert-butyl-p-cresol)In 2021, Olker, Jennifer H.;Korte, Joseph J.;Denny, Jeffrey S.;Haselman, Jonathan T.;Hartig, Phillip C.;Cardon, Mary C.;Hornung, Michael W.;Degitz, Sigmund J. published 《In vitro screening for chemical inhibition of the iodide recycling enzyme, iodotyrosine deiodinase》. 《Toxicology In Vitro》published the findings. The article contains the following contents:
The iodide recycling enzyme, iodotyrosine deiodinase (IYD), is a largely unstudied mol. mechanism through which environmental chems. can potentially cause thyroid disruption. This highly conserved enzyme plays an essential role in maintaining adequate levels of free iodide for thyroid hormone synthesis. Thyroid disruption following in vivo IYD inhibition has been documented in mammalian and amphibian models; however, few chems. have been tested for IYD inhibition in either in vivo or in vitro assays. Presented here are the development and application of a screening assay to assess susceptibility of IYD to chem. inhibition. With recombinant human IYD enzyme, a 96-well plate in vitro assay was developed and then used to screen over 1800 unique substances from the U. S. EPA ToxCast screening library. Through a tiered screening approach, 194 IYD inhibitors were identified (inhibited IYD enzyme activity by 20% or greater at target concentration of 200μM). 154 chems. were further tested in concentration-response (0.032-200μM) to determine IC50 and rank-order potency. This work broadens the coverage of thyroid-relevant mol. targets for chem. screening, provides the largest set of chems. tested for IYD inhibition, and aids in prioritizing chems. for targeted in vivo testing to evaluate thyroid-related adverse outcomes. The experimental procedure involved many compounds, such as 2,2-Methylenebis(6-tert-butyl-p-cresol) (cas: 119-47-1) .
Name: 2,2-Methylenebis(6-tert-butyl-p-cresol)2,2′-Methylenebis(4-methyl-6-tert-butylphenol)(CAS: 119-47-1) is a natural product found in Streptomyces and Aspergillus fumigatus .
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