Taritla, Sidhartha;Kumari, Madhuree;Kamat, Siya;Bhat, Sarita G.;Jayabaskaran, C. published 《Optimization of physicochemical parameters for production of cytotoxic secondary metabolites and apoptosis induction activities in the culture extract of a marine algal-derived endophytic fungus Aspergillus sp.》. The research results were published in《Frontiers in Pharmacology》 in 2021.Recommanded Product: 2-Hexyl-1-decanol The article conveys some information:
The endophytic fungal community in the marine ecosystem has been demonstrated to be relevant source of novel and pharmacol. active secondary metabolites. The current study focused on the evaluation of cytotoxic and apoptosis induction potential in the culture extracts of endophytic fungi associated with Sargassum muticum, a marine brown alga. The cytotoxicity of the four marine endophytes, Aspergillus sp., Nigrospora sphaerica, Talaromyces purpureogenus, and Talaromyces stipitatus, was evaluated by the MTT assay on HeLa cells. Further, several physicochem. parameters, including growth curve, culture media, and organic solvents, were optimized for enhanced cytotoxic activity of the selected extract The Aspergillus sp. Et acetate extract (ASE) showed maximum cytotoxicity on multiple cancer cell lines. Chem. investigation of the metabolites by gas chromatog.-mass spectroscopy (GC-MS) showed the presence of several compounds, including quinoline, indole, 2,4-bis(1,1-dimethylethyl) phenol, and hexadecenoic acid, known to be cytotoxic in ASE. The ASE was then tested for cytotoxicity in vitro on a panel of six human cancer cell lines, namely, HeLa (cervical adenocarcinoma), MCF-7 (breast adenocarcinoma), Hep G2 (hepatocellular carcinoma), A-549 (lung carcinoma), A-431 (skin/epidermis carcinoma), and LN-229 (glioblastoma). HeLa cells were most vulnerable to ASE treatment with an IC50 value of 24 ± 2 μg/mL. The mechanism of cytotoxicity exhibited by the ASE was further investigated on Hela cells. The results showed that the ASE was capable of inducing apoptosis in HeLa cells through production of reactive oxygen species, depolarization of mitochondrial membrane, and activation of the caspase-3 pathway, which shows a possible activation of the intrinsic apoptosis pathway. It also arrested the HeLa cells at the G2/M phase of the cell cycle, eventually leading to apoptosis. Through this study, we add to the knowledge about the marine algae associated with fungal endophytes and report its potential for purifying specific compounds responsible for cytotoxicity.2-Hexyl-1-decanol (cas: 2425-77-6) were involved in the experimental procedure.
2-Hexyl-1-decanol(cas: 2425-77-6) is a fatty acid that is found in the essential oils of plants and has been shown to have fungicidal properties.Recommanded Product: 2-Hexyl-1-decanol It has also been shown to inhibit the growth of Candida glabrata when used as a cationic surfactant.
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