Wie, Siong I.; Sylwester, Alan P.; Wing, Keith D.; Hammock, Bruce D. published the article 《Synthesis of haptens and potential radioligands and development of antibodies to insect growth regulators diflubenzuron and BAY SIR 8514》. Keywords: immunochem analysis diflubenzuron BAYSIR8514; hapten radioligand insect growth regulator.They researched the compound: 1-(4-Chlorophenyl)pyrrolidin-2-one( cas:7661-33-8 ).HPLC of Formula: 7661-33-8. Aromatic heterocyclic compounds can be divided into two categories: single heterocyclic and fused heterocyclic. In addition, there is a lot of other information about this compound (cas:7661-33-8) here.
A variety of synthetic approaches were undertaken, leading to potential haptens and radioligands for the benzoylphenylurea insect growth regulators diflubenzuron [35367-38-5] and BAY SIR 8514 [64628-44-0]. One successful approach involved derivatization of the aniline N by Et 4-bromobutyrate followed by reaction with an appropriate isocyanate and cleavage of the Et ester to yield a free carboxypropyl “”handle””. Useful haptens were also synthesized by using a 3′-phenolic metabolite of diflubenzuron as well as acetate and amine functionalities in the 4′ position while the N-sulfenyl bond proved too unstable for use as an antigen. With the exception of the sulfenylated derivatives, the haptens lacked significant bol. activity on 3 insect species. Following protein coupling by the active ester or water-soluble diimide method, antibodies were raised to 2 diflubenzuron haptens in each of 7 rabbits immunized as demonstrated by radioimmunoassay using [14C]diflubenzuron, Ouchterlony gel diffusion, and immunoelectrophoresis.
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