Reference of 10488-69-4 ,Some common heterocyclic compound, 10488-69-4, molecular formula is C6H11ClO3, its traditional synthetic route has been very mature, but the traditional synthetic route has various shortcomings, such as complicated route, low yield, poor purity, etc., below Introduce a new synthetic route.
Example 14: Preparation of Ethyl (R)-4-CYANO-3-HYDROXYYRATE FROM ETHYL (S)-4-CHLORO-3- hydroxybutyrate To a 250 mL vessel connected to an automatic titrater by a pH electrode and a feeding tube for addition of base (7.5 M NaOH) was charged water (83.5 mL) and 0.7 g of halohydrin dehalogenase SEQ ID NO: 24. The mixture was stirred for 30 minutes. The titrater was activated and set to maintain pH 7. Then, 25percent aqueous HCN (9.26 ml, 8.6 g) was charged over 20 minute to make A 2.3percent HCN solution. The mixture was heated at 40 °C for 10 minutes, then ethyl (S) -4-chloro-3-hydroxybutyrate (5 g) was charged over 1 hour. The automatic titrater maintained the pH at 7 by the addition of 2M NaOH. After 20 hrs, GC analysis of a butyl acetate extract of a reaction sample showed the conversion of the ethyl (S)-4-CHLORO-3-HYDROXYBUTYRATE to ethyl (R)-4-CYANO-3-HYDROXYBUTYRATE was 95percent.
These compound has a wide range of applications. It is believed that with the continuous development of the source of the synthetic route,10488-69-4, its application will become more common.
Reference:
Patent; CODEXIS, INC.; WO2005/18579; (2005); A2;,
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