Qualitative and quantitative analysis of the major components in Qinghao Biejia decoction by UPLC-Orbitrap Fusion-MS/MS and UPLC-QQQ-MS/MS and evaluation of their antibacterial activities was written by Cheng, Xin;Li, Biao-Ping;Han, Zhong-Xiao;Zhang, Feng-Lin;Jiang, Zhi-Rui;Yang, Jia-Shun;Luo, Qi-Zhi;Tang, Ling. And the article was included in Phytochemical Analysis in 2022.COA of Formula: C18H32CaN2O10 This article mentions the following:
In the present study, the chem. components of Qinghao Biejia decoction (QBD) were qual. and quant. analyzed using UPLC-Orbitrap Fusion-MS/MS and UPLC-QQQ-MS/MS techniques, followed by identification of each components origin and evaluation of the antibacterial activity of QBD and its components. High-resolution mass spectrometry was used to obtain information on the precise mol. weight, retention time, and fragmentation ion peaks of the compounds used to identify the components of QBD and establish a method for their quantification. In vitro assays including determination of the minimal inhibitory concentration and growth curves were used to assess the antibacterial activity of QBD and its components. A total of 39 components, including fatty acids, phenolic acids, amino acids, flavonoids, coumarins, terpenoids, and alkaloids, were identified by UPLC-Orbitrap Fusion-MS/MS. A high-performance anal. method was also established to quantify 12 components of QBD. The content of mangiferin was relatively high (estimated to be 814μg/g). The results of the antibacterial assays indicated that mangiferin exhibits antibacterial effects against two strains causing respiratory tract infections. The present study suggests that mangiferin may serve as a natural compound which shows high antibacterial activity. The results can aid the discovery and anal. of the active antimicrobial components present in QBD and further provide a reference for quality assessment of multi-component herbal prescriptions. In the experiment, the researchers used many compounds, for example, Calcium (R)-3-(2,4-dihydroxy-3,3-dimethylbutanamido)propanoate (cas: 137-08-6COA of Formula: C18H32CaN2O10).
Calcium (R)-3-(2,4-dihydroxy-3,3-dimethylbutanamido)propanoate (cas: 137-08-6) belongs to alcohols. Alkyl halides are often synthesized from alcohols, in effect substituting a halogen atom for the hydroxyl group. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.COA of Formula: C18H32CaN2O10
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts