Ex vivo, in vitro, and in silico approaches to unveil the mechanisms underlying vasorelaxation effect of Mentha Longifolia (L.) in porcine coronary artery was written by Alamgeer;Asif, Hira;Chohan, Tahir Ali;Irfan, Hafiz Muhammad;Asim, Mulazim Hussain;Bukhari, Syed Nasir Abbas;Younis, Waqas;Althobaiti, Yusuf S.;Ullah, Aman;Khan, Abdul Qayyum;Hakami, Alqassem Y.. And the article was included in Biomedicine & Pharmacotherapy in 2022.Quality Control of 5-Isopropyl-2-methylphenol This article mentions the following:
Mentha (M.) longifolia (L.) is traditionally used for various ailments. The current study was intended to explore the underlying vasorelaxation mechanisms of M. longifolia. Aqueous-methanol extract from the aerial parts of M. longifolia was prepared and subjected to activity-guided fractionation. The vasorelaxant activity was performed using porcine coronary arteries with intact and denuded endothelium. In-vitro PDE inhibitory activity of the active fraction was carried out using the radio-enzymic assay. The active fraction was also subjected to GCMS. Docking and mol. dynamic simulation studies were also performedWe had observed that aqueous-methanolic extract induced relaxation in the coronary artery in a dose-dependent manner when the endothelium was intact and denuded. n-butanol fraction (MLB) has produced a maximum effect, and it was selected for mechanistic studies. MLB has significantly enhanced the relaxation produced by cAMP and cGMP, elevating atrial natriuretic peptide, sodium nitroprusside, isoproterenol, and forskolin. The pre-treatment with MLB inhibited the contractile response produced by KCl, U46619, and CaCl2 in without endothelium rings. MLB has non-selectively inhibited the PDE isoforms. GCMS anal. of MLB has revealed the presence of menthol, thymol, and carvacrol in the active fraction. Docking and mol. dynamic simulation studies have indicated that thymol can be a competitive inhibitor for PDE1. It is postulated that an n-butanol fraction of Mentha longifolia produced endothelium-independent relaxation due to increased levels of cAMP and cGMP caused by the inhibition of various PDEs. In the experiment, the researchers used many compounds, for example, 5-Isopropyl-2-methylphenol (cas: 499-75-2Quality Control of 5-Isopropyl-2-methylphenol).
5-Isopropyl-2-methylphenol (cas: 499-75-2) belongs to alcohols. Alkyl halides are often synthesized from alcohols, in effect substituting a halogen atom for the hydroxyl group. Under carefully controlled conditions, simple alcohols can undergo intermolecular dehydration to give ethers. This reaction is effective only with methanol, ethanol, and other simple primary alcohols.Quality Control of 5-Isopropyl-2-methylphenol
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts