Preparation of combined cross-linked enzyme aggregates and its application in synthesis of chiral alcohols by the asymmetric reduction of carbanyl group was written by Yang, Meng;Jiang, Huijuan;Ning, Chenxi;Wei, Dongzhi;Su, Erzheng. And the article was included in Gaodeng Xuexiao Huaxue Xuebao in 2018.Formula: C8H9ClO This article mentions the following:
Using ketoreductase KRED 30 from Corynebacterium sp. and the D-glucose dehydrogenase from Bacillus subtilis as the model enzymes, a combined cross-linked enzyme aggregates (combi-CLEAs) with high activity was prepared by optimization of the precipitant type, glutaraldehyde concentration, crosslinking temperature and time. Characterization of the combi-CLEAs showed that the stability and substrate tolerance of free enzymes mixture were significantly improved after combined crosslinking. Investigation of catalytical performance showed that combi-CLEAs could effectively catalyze the reduction of Et 4-chloroacetoacetate (COBE) and 3′-chloroacetophenone (CPO) for the synthesis of corresponding chiral alcs. in aqueous system. Only a little cofactor was needed to start the reactions. The catalytical activity could retain more than 70% after continuously catalyzing for 10 batches. The result showed that catalytical performance of combi-CLEAs in the biphasic system was much better than that in aqueous system. The total turnover number (TTN) for catalyzing COBE could attend 6595, and TTN for catalyzing CPO could attend 7500. Thus, cofactor regeneration by combi-CLEAs is feasible. In the experiment, the researchers used many compounds, for example, (R)-1-(3-Chlorophenyl)ethanol (cas: 120121-01-9Formula: C8H9ClO).
(R)-1-(3-Chlorophenyl)ethanol (cas: 120121-01-9) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.Formula: C8H9ClO
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts