Fit-for-purpose characterization of air-liquid-interface (ALI) in vitro exposure systems for e-vapor aerosol was written by Zhang, J.;Doshi, U.;Wolz, R. L.;Kosachevsky, P.;Oldham, M. J.;Gillman, I. G.;Lee, K. M.. And the article was included in Toxicology In Vitro in 2022.Formula: C3H8O2 This article mentions the following:
Air-liquid-interface (ALI) exposure systems deliver aerosol to the apical surface of cells which mimics the in vivo inhalation exposure conditions. It is necessary, however, to quantify the delivered amount of aerosol for ALI-based in vitro toxicity assessment. In this study, we evaluated two com. available ALI exposure systems, a Vitrocell Ames 48 (Ames 48) and a Vitrocell 24/48 (VC 24/48), and the Vitrocell VC1/7 smoking machine using a cig-a-like cartridge-based e-vapor device with a prototype formulation (containing 4% nicotine by weight). We characterized aerosol particle-size distribution, aerosol mass, and major chem. components (nicotine, propylene glycol, and glycerol) at the generation source and verified the repeatability of the aerosol generation. We determined aerosol delivery at the ALI by gravimetric anal. of mass collected on Cambridge filter pads and anal. quantitation of the buffer medium which showed that both aerosol mass and nicotine to an exposure insert linearly increased up to 400 puffs. The delivered aerosol mass covered a wide range of 0.8-3.4 mg per insert in the Ames 48 with variability (relative standard deviation, RSD) up to 12% and 1.1-6.4 mg per insert in the VC 24/48 with variability up to 15%. The delivered nicotine ranged approx. up to 200 μg per insert in both exposure systems. These results provided operation and aerosol delivery information of these ALI exposure systems for subsequent in vitro testing of e-vapor aerosols. In the experiment, the researchers used many compounds, for example, 1,2-Propanediol (cas: 57-55-6Formula: C3H8O2).
1,2-Propanediol (cas: 57-55-6) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Formula: C3H8O2
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts