Multiple cryoprotectant toxicity model for vitrification solution optimization was written by Warner, Ross M.;Brown, Kevin S.;Benson, James D.;Eroglu, Ali;Higgins, Adam Z.. And the article was included in Cryobiology in 2022.Reference of 57-55-6 This article mentions the following:
Vitrification is a promising cryopreservation technique for complex specimens such as tissues and organs. However, it is challenging to identify mixtures of cryoprotectants (CPAs) that prevent ice formation without exerting excessive toxicity. In this work, we developed a multi-CPA toxicity model that predicts the toxicity kinetics of mixtures containing five of the most common CPAs used in the field (glycerol, di-Me sulfoxide (DMSO), propylene glycol, ethylene glycol, and formamide). The model accounts for specific toxicity, non-specific toxicity, and interactions between CPAs. The proposed model shows reasonable agreement with training data for single and binary CPA solutions, as well as ternary CPA solution validation data. Sloppy model anal. was used to examine the model parameters that were most important for predictions, providing clues about mechanisms of toxicity. This anal. revealed that the model terms for non-specific toxicity were particularly important, especially the non-specific toxicity of propylene glycol, as well as model terms for specific toxicity of formamide and interactions between formamide and glycerol. To demonstrate the potential for model-based design of vitrification methods, we paired the multi-CPA toxicity model with a published vitrification/devitrification model to identify vitrifiable CPA mixtures that are predicted to have minimal toxicity. The resulting optimized vitrification solution composition was a mixture of 7.4 m glycerol, 1.4 m DMSO, and 2.4 m formamide. This demonstrates the potential for math. optimization of vitrification solution composition and sets the stage for future studies to optimize the complete vitrification process, including CPA mixture composition and CPA addition and removal methods. In the experiment, the researchers used many compounds, for example, 1,2-Propanediol (cas: 57-55-6Reference of 57-55-6).
1,2-Propanediol (cas: 57-55-6) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Reference of 57-55-6
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts