Yuan, Bo published the artcileUHPLC-QqQ-MS/MS method development and validation with statistical analysis: Determination of raspberry ketone metabolites in mice plasma and brain, SDS of cas: 621-37-4, the publication is Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (2020), 122146, database is CAplus and MEDLINE.
Raspberry ketone (RK) (4-(4-hydroxyphenyl)-2-butanone) is the major compound responsible for the characteristic aroma of red raspberries, and has long been used com. as a flavoring agent and recently as a weight loss supplement. A targeted UHPLC-QqQ-MS/MS method was developed and validated for anal. of RK and 25 associated metabolites in mouse plasma and brain. Dispersion and projection anal. and central composite design were used for method optimization. Random effect anal. of variance was applied for validation inference and variation partition. Within this framework, repeatability, a broader sense of precision, was calculated as fraction of accuracy variance, reflecting instrumental imprecision, compound degradation and carry-over effects. Multivariate correlation anal. and principle component anal. were conducted, revealing underlying association among the manifold of method traits. R programming was engaged in streamlined statistical anal. and data visualization. Two particular phenomena, the analytes’ background existence in the enzyme solution used for phase II metabolites deconjugation, and the noted lability of analytes in pure solvent at 4° vs. elevated stability in biomatrices, were found critical to method development and validation. The approach for the method development and validation provided a foundation for experiments that examine RK metabolism and bioavailability.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about 621-37-4. 621-37-4 belongs to alcohols-buliding-blocks, auxiliary class Carboxylic acid,Benzene,Phenol,Natural product, name is 3-Hydroxyphenylacetic acid, and the molecular formula is C11H15BO4, SDS of cas: 621-37-4.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
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