Colin, Camille published the artcileSingle-particle tracking method in fluorescence microscopy to monitor bioenergetic responses of individual mitochondria, Related Products of alcohols-buliding-blocks, the main research area is single particle tracking mitochondrial permeability transition pore fluorescence microscopy; Bioenergetics; Fiji software; Fluorescence microscopy; Membrane potential; Mitochondria; Single organelle; Single particle tracking; TrackMate.
The spectroscopic methods commonly used to study mitochondria bioenergetics do not show the diversity of responses within a population of mitochondria (isolated or in a cell), and/or cannot measure individual dynamics. New methodol. developments are necessary in order to improve quant. and kinetic resolutions and eventually gain further insights on individual mitochondrial responses, such as studying activities of the mitochondrial permeability transition pore (mPTP). The work reported herein is devoted to study responses of single mitochondria within a large population after isolation from cardiomyocytes. Mitochondria were preloaded with a commonly used membrane potential sensitive dye (TMRM), they are then deposited on a plasma-treated glass coverslip and subsequently energized or inhibited by additions of usual bioenergetics effectors. Responses were analyzed by fluorescence microscopy over few thousands of mitochondria simultaneously with a single organelle resolution We report an automatic method to analyze each image of time-lapse stacks based on the TrackMate-ImageJ plug-in and specially made Python scripts. Images are processed to eliminate defects of illumination inhomogeneity, improving by at least two orders of magnitude the signal/noise ratio. This method enables us to follow the track of each mitochondrion within the observed field and monitor its fluorescence changes, with a time resolution of 400 ms, uninterrupted over the course of the experiment Such methodol. improvement is a prerequisite to further study the role of mPTP in single mitochondria during calcium transient loading.
Methods in Molecular Biology (New York, NY, United States) published new progress about Cardiomyocyte. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Related Products of alcohols-buliding-blocks.
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