Wang, Wenying published the artcileHemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection, Related Products of alcohols-buliding-blocks, the main research area is IPC cardiomyocyte protection hemichannel mediated volume regulation contribute; capacity regulation; hemichannels; ischemic preconditioning; myocardial protection.
Cx43 has been documented to be involved in ischemic preconditioning (IPC). However, the participation of Cx43-formed hemichannels in IPC and the potential underlying mechanisms remain unclear. The present study focused on cardiomyocytes’ volume regulation during IPC to investigate the role of hemichannels in the IPC-induced cardioprotection. In the study, mice cardiomyocytes were resp. treated with a hemichannel blocker, octanol or 18a-Glycyrrhizic acid (18a-GA), and a Cx43-silenced lentivirus. They were subsequently cultured in hypotonic solution to simulate ischemic reperfusion (SIR) and systemic ischemic preconditioning (SIP). Cell morphol. and volumetric (area) change were detected by inverted microscopy at 30 min following the addition of hypotonic solution Cardiomyocyte mortality was assessed by trypan blue stain assay. The analyses revealed that regardless of the treatments, hypotonic solution aggravated cell edema: Compared with the initial condition (the moment before the solution addition, 0 min), the volumetric area increased significantly 30 min later (for hypotonic + DMSO, 5,050 ± 1,511 vs. 3,464 ± 723μm2; for hypotonic+scramble lentiviral vector, 5,517 ± 1,128 vs. 2,331 ± 536μm2; P<0.05, resp.). Either treatment alleviated the edematous condition when a comparison was made between 30 min after the hypotonic addition and 0 min (for hypotonic+octanol, 2,990 ± 765 vs. 2,821 ± 773μm2; for hypotonic+18a-GA, 4,817 ± 1,306 vs. 4,762 ± 1,271μm2; for hypotonic + Cx43-silenced, 3,627 ± 688 vs. 3,419 ± 814μm2; P>0.05 for all). Notably, results indicated that the SIP group had lower mortality rates compared with its SIR counterpart; the hypotonic + octanol, hypotonic + 18a-GA, and hypotonic + Cx43-silenced group showed markedly-declined mortality when compared with their resp. control groups (resp., 35.70 ± 1.02, 30.76 ± 2.20 vs. 53.58 ± 2.14%; 30.89 ± 2.37 vs. 54.12 ± 2.55%; P<0.05 for all). The results suggest that ischemic preconditioning may provide cardioprotection by blocking the opening of the hemichannels and further mediating the volume regulation of cardiomyocytes. Experimental and Therapeutic Medicine published new progress about Cardiomyocyte. 111-87-5 belongs to class alcohols-buliding-blocks, name is n-Octanol, and the molecular formula is C8H18O, Related Products of alcohols-buliding-blocks.
Referemce:
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