Liu, Jia-Jia published the artcileGC-MS profile of Hua-Feng-Dan and RNA-Seq analysis of induced adaptive responses in the liver, Application of rel-(1R,2R,4R)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol, the main research area is Hua Feng Dan RNA sequence liver bioinformatic signaling GCMS; GC-MS; GEO database; Hua-Feng-Dan; RNA-seq; adaptation; bioinformatics.
Hua-Feng-Dan is a patent Chinese medicine for stroke recovery and various diseases. This study used GC-MS to profile its ingredients and RNA-Seq to analyze the induced adaptive response in the liver. Hua-Feng-Dan was subjected to steam distillation and solvent extraction, followed by GC-MS anal. Mice were orally administered Hua-Feng-Dan and its “”Guide drug”” Yaomu for 7 days. Liver pathol. was examined, and total RNA isolated for RNASeq, followed by bioinformatic anal. and quant. real-time PCR (qPCR). Forty-four volatile and fifty liposol. components in Hua-Feng-Dan were profiled and analyzed by the NIST library and their concentrations quantified. The major components (>1%) in volatile (5) and liposol. (10) were highlighted. HuaFeng-Dan and Yaomu at hepatoprotective doses did not produce liver toxicity as evidenced by histopathol. and serum enzyme activities. GO Enrichment revealed that Hua-Feng-Dan affected lipid homeostasis, protein folding, and cell adhesion. KEGG showed activated cholesterol metabolism, bile secretion, and PPAR signaling pathways. Differentially expressed genes (DEGs) were identified by DESeq2 with p < 0.05 compared to controls. Hua-Feng-Dan produced more DEGs than Yaomu. qPCR on selected genes largely verified RNA-Seq results. Ingenuity Pathways Anal. of the upstream regulator revealed activation of MAPK and adaptive responses by Hua-FengDan, and Yaomu was less effective. Hua-Feng-Dan-induced DEGs were highly correlated with the Gene Expression Omnibus database of chem.-induced adaptive transcriptome changes in the liver. GC-MS primarily profiled volatile and liposol. components in Hua-FengDan. Hua-Feng-Dan at the hepatoprotective dose did not produce liver pathol. changes but induced metabolic and signaling pathway activations. The effects of Hua Feng-Dan on liver transcriptome changes point toward induced adaptive responses to program the liver to produce hepatoprotective effects. Frontiers in Pharmacology published new progress about Bioinformatics. 124-76-5 belongs to class alcohols-buliding-blocks, name is rel-(1R,2R,4R)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol, and the molecular formula is C10H18O, Application of rel-(1R,2R,4R)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol.
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