Swain, Alaka published the artcileMorphophysiological alterations in transgenic rice lines expressing PPDK and ME genes from the C4 model Setaria italica, Computed Properties of 97-67-6, the main research area is Setaria transgenic plant morphophysiol alteration PPDK ME; Chlorophyll fluorescence; Electron transport rate; Malic enzyme; Nonphotochemical quenching; Photosynthetic rate; Pyruvate orthophosphate dikinase; Stomatal conductance.
To evaluate the effect of C4 enzymes in rice, we developed transgenic rice lines by sep. introducing Setaria italica PPDK [SiPPDK] and S. italica ME [SiME] gene constructs under the control of the green tissue-specific maize PPDK promoter. Rice plant lines for both constructs were screened using the polymerase chain reaction (PCR), Southern hybridization, and expression anal. The results from qRT-PCR and enzyme activity anal. revealed higher expression and activity of both PPDK and NADP-ME genes compared with the nontransformed and empty-vector-transformed plants. The average photosynthetic efficiency of transgenic plant lines carrying the PPDK and NADP-ME genes increased by 18% and 12%, resp., and was pos. correlated with the increased accumulation of photosynthetic pigment. The decrease in Fv/Fm, increased electron transport rate (ETR), and increased photochem. quenching (qP) compared with nontransformed control plants suggest that transgenic rice plants transferred more absorbed light energy to photochem. reactions than wild-type plants. SiME-transgenic plants displayed reduced leaf malate content and superior performance under water deficit conditions. Interestingly, the transgenic plants showed yield enhancement by exhibiting increased plant height, panicle length, panicle weight and thousand grain weight Overall, the exogenous foxtail millet C4 gene PPDK enhanced photosynthesis and yield to a greater extent than NADP-ME.
Journal of Plant Physiology published new progress about Cell morphology. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Computed Properties of 97-67-6.
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