Li, Yan-xiao published the artcilePreparation, characterization, and prebiotic activity of manno-oligosaccharides produced from cassia gum by a glycoside hydrolase family 134 β-mannanase, Synthetic Route of 59-23-4, the main research area is prebiotic mannooligosaccharide cassia gum mannanase; Cassia gum; Characterization; Manno-oligosaccharides; Prebiotic activity; β-Mannanase.
To produce mannooligosaccharides from cassia gum, a mutated glycoside hydrolase family 134 β-mannanase gene (mRmMan134A) from Rhizopus microsporus var. rhizopodiformis F518 was expressed in Pichia pastoris and a high expression level (3680 U mL-1) was obtained through high cell d. fermentation mRmMan134A exhibited maximum activity at pH 5.5 and 50°C. It was then subjected to hydrolyze cassia gum with 70.6% of overall yield of mannooligosaccharides. From the hydrolyzate, seven components (F1-F7) were separated and identified as mannose, mannobiose, galactose, mannotriose, mannotetraose, 61-α-D-galactosyl-β-D-mannobiose, and mannopentaose, resp. According to in vitro fermentation, the mannooligosaccharides were able to promote the growth of three Bifidobacterium strains and six Lactobacillus strains with 3.0-fold increment in culture absorbance, and these strains preferred mannooligosaccharides with DP 2-3 rather than those with DP 4-5. Novel mannooligosaccharides from cassia gum with promising prebiotic activity were provided in the present study.
Food Chemistry published new progress about Bifidobacterium. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Synthetic Route of 59-23-4.
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