Dreiling, Alena published the artcileEarly cleavage of ethylene glycol bis(succinimidylsuccinate) (EGS)-linker moieties during enzymatic digestion of cross-linked proteins, Quality Control of 70539-42-3, the publication is Rapid Communications in Mass Spectrometry (2014), 28(21), 2385-2388, database is CAplus and MEDLINE.
It has become popular to combine the crosslinking of proteins with mass spectrometry (MS) to study their tertiary structure or interactions. There are several difficulties with the method which make it a challenge. They include the limited availability of crosslinked peptides for MS anal. due to low yield of the crosslinking reaction and unwanted side chem. Recently, the authors have pointed out the uncontrolled quenching of azide linkers by buffer components rendering them almost useless for the intended task. Here, the authors describe a phenomenon involving cleavable crosslinkers. They have been designed to overcome the drawback that crosslinked peptides provide poor gas-phase fragmentation mass spectra because of their branched structure. Moreover, isotope coding on such linkers may assist in finding the low-abundance crosslinked peptides in mass spectra as described for ethylene glycol bis(succinimidylsuccinate) (EGS). In some of the authors’ experiments, this compound provided better crosslinking yields compared to other N-hydroxysuccinimide-derived reagents as detected by one-dimensional sodium dodecyl sulfate PAGE. For best results from crosslinking experiments using cleavable agents such as EGS, careful control of sample preparation protocols for early hydrolysis products is advisable.
Rapid Communications in Mass Spectrometry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Quality Control of 70539-42-3.
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