Segnitz, Bernd published the artcileSubunit structure of the nonactivated human estrogen receptor, COA of Formula: C18H20N2O12, the publication is Proceedings of the National Academy of Sciences of the United States of America (1995), 92(6), 2179-83, database is CAplus and MEDLINE.
The nonactivated estrogen receptor of human MCF-7 mammary carcinoma cells was investigated with respect to stoichiometry of protein subunits. The native receptor complex stabilized by molybdate had a mol. mass of ≈300 kDa. Chem. crosslinking with several bifunctional reagents resulted in complete stabilization of the same receptor form of ≈300 kDa and was achieved both in cell extracts and in intact cells. Incubation of the cross-linked receptor with a receptor-specific monoclonal IgG1 antibody increased the mol. mass by ≈135 kDa-i.e., no more than one Ig mol. bound to the complex. Partial and progressive crosslinking of affinity-labeled receptors revealed patterns of labeled bands upon denaturing gel electrophoresis indicative of a heteromeric structure. The completely cross-linked receptor was purified to homogeneity and analyzed for protein components. In addition to the receptor polypeptide of ≈65 kDa, the authors detected the heat shock proteins hsp90 and p59; the hsp90 band was roughly twice as intense as the p59 band. The heat shock protein hsp70 and the 40-kDa cyclophilin were not detected as components of the highly purified cross-linked receptor of ≈300 kDa. The authors suggest a heterotetrameric structure consisting of one receptor polypeptide, two hsp90 mols., and one p59 subunit, for which the mol. mass adds up to ≈300 kDa. Thus, the nonactivated estrogen receptor has a mol. architecture homologous to those of glucocorticoid and progesterone receptors, even though phylogenetically the estrogen receptor gene forms a distinct subgroup within the gene family of nuclear hormone receptors.
Proceedings of the National Academy of Sciences of the United States of America published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C12H6NNaO4, COA of Formula: C18H20N2O12.
Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts