Lu, Shu-xia published the artcileOptimization of medium composition for two-step fermentation of vitamin C using response surface methodology, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Shipin Kexue (Beijing, China) (2013), 34(3), 202-206, database is CAplus.
2-Keto-L-gulonic acid production during the conversion process from L-sorbose to 2-keto-L-gulonic acid in the two-step fermentation of vitamin C was improved. An efficient companion strain of Bacillus subtilis A9 that facilitates the growth of Gluconobacter oxydans and the production of acids was screened from fresh milk. Based on single factor tests, the compositions of fermentation medium were optimized by response surface methodol. The results showed that three factors including L-sorbose, corn liquor and urea revealed significant impact on the production of 2-KGA. The optimal compositions included 90 g/L L-sorbose, 14.2 g/L corn liquor, 12.2 g/L urea, 4.1 g/L CaCO3 and 0.2 g/L MgSO4. Under the optimal medium, the yield of 2-keto-L-gulonic acid was up to 69.74 g/L, which was close to the theor. value of 70.33 g/L with the relative error of-0.59 g/L. The established regression equation for 2-KGA revealed excellent fitting. Therefore, it is credible and can be used for practical prediction.
Shipin Kexue (Beijing, China) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.
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