Rauter, Marion published the artcileSynthesis of benzyl β-D-galactopyranoside by transgalactosylation using a β-galactosidase produced by the over expression of the Kluyveromyces lactis LAC4 gene in Arxula adeninivorans, Related Products of alcohols-buliding-blocks, the main research area is benzyl beta galactopyranoside transgalactosylation galactosidase Kluyveromyces LAC4 Arxula.
The LAC4 gene of Kluyveromyces lactis encoding for β-galactosidase was overexpressed in the yeast Arxula adeninivorans to produce the enzyme, which can be used for the synthesis of β-D-galactosides. These compounds play a major role as precursors for the synthesis of glycolipids and glycoproteins in medicine or for the production of tensides. The Xplor2 transformation/expression platform was used because it enabled stable integration of the gene in the Arxula genome and the production of high levels of the enzyme. The recombinant β-galactosidase, fused with C-terminal His-tag region (Lac4-6hp), was purified by precipitation with ammonium sulfate and FPLC using hydroxylapatite. The enzyme exhibited optimal activity at 37 to 40°, pH 6.5 in 50 mM sodium acetate buffer. Activity was measured by the formation of p-nitrophenol at 405 nm from the hydrolyzed chromogenic substrate, p-nitrophenyl-β-D-gal. Biochem. characterization included the calculation of Km and apparent kcat values of the enzyme. The formation of benzyl β-D-gal by 0.1 U enzyme from A. adeninivorans with transgalactosylation was six times higher than that for the prokaryotic enzyme from E. coli. Moreover, the partially purified enzyme was used for the selective hydrolysis of allyl β-D-gal in a mixture of allyl β- and allyl α-D-gal, with 4 g l-1 being hydrolyzed within one day by 1 U ml-1. Thus, the recombinant β-galactosidase produced in A. adeninivorans is of potential interest for the enzymic synthesis of benzyl β-D-gal and other galactosides as well as the selective hydrolysis of anomeric mixtures and could be used to replace difficult chem. procedures.
Journal of Molecular Catalysis B: Enzymatic published new progress about Anomers. 2595-07-5 belongs to class alcohols-buliding-blocks, name is (2R,3R,4S,5R,6R)-2-(Allyloxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol, and the molecular formula is C9H16O6, Related Products of alcohols-buliding-blocks.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts