Zhang, Chen published the artcileExtract methods, molecular characteristics, and bioactivities of polysaccharide from alfalfa (Medicago sativa L.), Quality Control of 59-23-4, the main research area is Medicago polysaccharide bioactivity; compositions; glycosidic linkage; immunomodulation; molecular weight; polysaccharide.
The polysaccharide isolated from alfalfa was considered to be a kind of macromol. with some biol. activities; however, its mol. structure and effects on immune cells are still unclear. The objectives of this study were to explore the extraction and purifying methods of alfalfa (Medicago sativa L.) polysaccharide (APS) and decipher its composition and mol. characteristics, as well as its activation to lymphocytes. The crude polysaccharides isolated from alfalfa by water extraction and alc. precipitation methods were purified by semipermeable membrane dialysis. Five batches of alfalfa samples were obtained from five farms (one composite sample per farm) and three replicates were conducted for each sample in determination The results from ion chromatog. (IC) anal. showed that the APS was composed of fucose, arabinose, galactose, glucose, xylose, mannose, galactose, galacturonic acid (GalA), and glucuronic acid (GlcA) with a molar ratio of 2.6:8.0:4.7:21.3:3.2:1.0:74.2:14.9. The weight-average mol. weight (Mw), number-average mol. weight (Mn), and Z-average mol. weight (Mz) of APS were calculated to be 3.30 × 106, 4.06 × 105, and 1.43 × 108 g/mol, resp., according to the anal. by gel permeation chromatog.-refractive index-multiangle laser light scattering (GPC-RI-MALS). The findings of electron ionization mass spectrometry (EI-MS) suggest that APS consists of seven linkage residues, namely 1,5-Araf, galactose (T-D-Glc), glucose (T-D-Gal), 1,4-Gal-Ac, 1,4-Glc, 1,6-Gal, and 1,3,4-GalA, with molar proportions of 10.30%, 4.02%, 10.28%, 52.29%, 17.02%, 3.52%, and 2.57%, resp. Addnl., APS markedly increased B-cell proliferation and IgM secretion in a dose- and time-dependent manner but not the proliferation and cytokine (IL-2, -4, and IFN-γ) expression of T cells. Taken together, the present results suggest that APS are macromol. polymers with a molar mass (indicated by Mw) of 3.3 × 106 g/mol and may be a potential candidate as an immunopotentiating pharmaceutical agent or functional food.
Nutrients published new progress about Alfalfa. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Quality Control of 59-23-4.
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