Kotidis, Pavlos published the artcileModel-based optimization of antibody galactosylation in CHO cell culture, Computed Properties of 59-23-4, the main research area is ovary cellular growth antibody galactosylation optimization math modeling; Chinese hamster ovary (CHO) cells; antibody glycosylation; galactosylation; mathematical modeling; nucleotide sugars; process optimization.
Exerting control over the glycan moieties of antibody therapeutics is highly desirable from a product safety and batch-to-batch consistency perspective. Strategies to improve antibody productivity may compromise quality, while interventions for improving glycoform distribution can adversely affect cell growth and productivity. Process design therefore needs to consider the trade-off between preserving cellular health and productivity while enhancing antibody quality. In this work, we present a modeling platform that quantifies the impact of glycosylation precursor feeding – specifically that of galactose and uridine – on cellular growth, metabolism as well as antibody productivity and glycoform distribution. The platform has been parameterized using an initial training data set yielding an accuracy of ±5% with respect to glycoform distribution. It was then used to design an optimized feeding strategy that enhances the final concentration of galactosylated antibody in the supernatant by over 90% compared with the control without compromising the integral of viable cell d. or final antibody titer. This work supports the implementation of Quality by Design towards higher-performing bioprocesses.
Biotechnology and Bioengineering published new progress about Cell density. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Computed Properties of 59-23-4.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts