Sran, Kulwinder Singh published the artcileProduction, characterization and bio-emulsifying activity of a novel thermostable exopolysaccharide produced by a marine strain of Rhodobacter johrii CDR-SL 7Cii, Safety of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is exopolysaccharide Rhodobacter johrii fermentation; Bio-emulsifier; Exopolysaccharide; Rhodobacter johrii; Structural characterization.
An exopolysaccharide (EPS) producing bacterial strain was isolated from the surface of marine macroalgae (Padina sp.). Based on polyphasic taxonomy, the strain CDR-SL 7Cii was assigned to the genus Rhodobacter and found to be the closest relative of the species Rhodobacter johrii. The strain was able to produce 6.2 g/l of EPS upon fermentation using R2A medium enriched with 2.5% glucose. FT-IR anal. revealed the presence of hydroxyl, carboxyl and diacyl-ester functional groups in the purified EPS. Further Chromatog. study revealed that R. johrii synthesized a high mol. weight anionic exopolysaccharide composed of glucose, glucuronic acid, rhamnose and galactose in a molar ratio of 3:1.5:0.25:0.25. The 1D and 2D NMR spectroscopy (COSY/HSQC) anal. revealed the presence of 1,6 linked-α-D-Glcp, 1,4 linked-β-D-Glcp, 1,3 linked-β-D-GlcA, 1,3 linked-β-D-Galp, 1,6 linked-β-D-Galf and 3-α-L-Rhmp residues. Moreover, the purified EPS has shown stability towards elevated temperature and also acted as a bio-emulsifier to create a high pH and temperature stable emulsion of hydrocarbon/water indicating its industrial potential. This is the first report of EPS production by a strain of Rhodobacter johrii.
International Journal of Biological Macromolecules published new progress about Exopolysaccharides Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Safety of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.
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