Waris, Muhammad Irfan published the artcileMolecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH, Synthetic Route of 584-02-1, the main research area is mol in vitro biochem assessment chemosensory protein; Nilaparvata lugens acidic pH.
Chemosensory proteins (CSPs) are important mol. components of the insect olfactory system, which are involved in capturing, binding, and transporting hydrophobic odor mols. across the sensillum in sensillar lymph in regulating insect behavior. This protein family (CSPs) is also involved in many other systems that are not linked to olfactory receptors in olfactory sensilla. The brown planthopper (BPH) is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by viruses. In this study, fluorescence competitive binding assay and fluorescence quenching assay at acidic pH were performed as well as homol. modeling to describe the binding affinity of NlugCSP10. Fluorescence competitive binding assay (FCBA) demonstrated that NlugCSP10 bound strongly to nonadecane, farnesene, and 2-tridecanone at acidic pH. The results of FCBA indicated that NlugCSP10 bound different ligands at the physiol. pH (5.0) of the bulk sensillum lymph. Fluorescence quenching assay demonstrated that NlugCSP10 generated a stable complex with 2-tridecanone, while two ligands nonadecane and farnesene collided due to mol. collisions. The interaction of selected ligands with the modeled structure of NlugCSP10 was also analyzed, which found the key amino acids (Gln23, Gln24, Gln25, Asn27, Met33, Ser34, Ile35, Tyr36, Asn42, Met43, Val45, Asn46, Asn93, Arg96, Ala97, Lys99, and Ala100) in NlugCSP10 that were involved in binding of volatile compounds The present study contributes to the binding profile of NlugCSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.
Journal of Integrative Agriculture published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (Ala97). 584-02-1 belongs to class alcohols-buliding-blocks, name is 3-Pentanol, and the molecular formula is C5H12O, Synthetic Route of 584-02-1.
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