Rossiter, Nicholas J. published the artcileCRISPR screens in physiologic medium reveal conditionally essential genes in human cells, Formula: C4H6O5, the main research area is human essential gene physiol medium CRISPR screen; CRISPR; HPLM; conditional gene essentiality; gene-nutrient interaction; genetic screen; physiologic medium.
Forward genetic screens across hundreds of cancer cell lines have started to define the genetic dependencies of proliferating human cells and how these vary by genotype and lineage. Most screens, however, have been carried out in culture media that poorly reflect metabolite availability in human blood. Here, we performed CRISPR-based screens in traditional vs. human plasma-like medium (HPLM). Sets of conditionally essential genes in human cancer cell lines span several cellular processes and vary with both natural cell-intrinsic diversity and the combination of basal and serum components that comprise typical media. Notably, we traced the causes for each of three conditional CRISPR phenotypes to the availability of metabolites uniquely defined in HPLM vs. conventional media. Our findings reveal the profound impact of medium composition on gene essentiality in human cells, and also suggest general strategies for using genetic screens in HPLM to uncover new cancer vulnerabilities and gene-nutrient interactions.
Cell Metabolism published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (EIF3A). 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Formula: C4H6O5.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts