Cavicchioli, Valeria Quintana published the artcilePhysiological and molecular insights of bacteriocin production by Enterococcus hirae ST57ACC from Brazilian artisanal cheese, SDS of cas: 97-67-6, the main research area is Enterococcus hirae physiol bacteriocin production Brazilian artisanal cheese; ABC transporter; Enterococcus hirae; bacteriocin; cytotoxicity.
The bacteriocinogenic Enterococcus hirae ST57ACC recently isolated from a Brazilian artisanal cheese was subjected here to addnl. analyses in order to evaluate its bacteriocin production and the potential influence of ABC transporter system in its expression. Besides these physiol. and mol. aspects, the bacteriocin was evaluated for its cytotoxicity against HT-29. Differences in the inoculum size had no impact on the growth of E. hirae ST57ACC; however, the bacteriocin was only produced after 9 h of growth when the strain was inoculated at 5% or 10% (volume/volume), with similar levels of bacteriocin production obtained by both conventional growth and batch fermentation Furthermore, potential expression of ABC transporters corresponding to the bacteriocin transport and sugar metabolism was identified. In terms of adverse effects, when a semi-purified fraction of the bacteriocin and the cell-free supernatant were tested against HT-29, total cell viability was similar to observed on untreated cells, indicating the absence of cytotoxic effect. Based on the obtained results, E. hirae ST57ACC can produce its bacteriocin at industrial level by using bioreactors, its bacteriocin expression is potentially influenced by the ABC transporter system, and no cytotoxic effects were observed on HT-29 cells, indicating its potential use as a bio-preservative.
Brazilian Journal of Microbiology published new progress about Bacteriocins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, SDS of cas: 97-67-6.
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