Kim, Jungyeon published the artcileSystematic re-evaluation of the long-used standard protocol of urease-dependent metabolome sample preparation, SDS of cas: 64519-82-0, the main research area is urease urea urinary metabolite metabolomic human mass spectrometry.
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatog.-based metabolomic anal., urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary metabolomics until today even for mass spectrometry-based anal. The urease pretreatment involving incubation of urine with urease contradicts the concept of metabolome sampling, which should immediately arrest metabolic reactions to prevent alterations of a metabolite profile. Nonetheless, the impact of urease pretreatment has not been clearly elucidated yet. We found that activities of urease and endogenous urinary enzymes and metabolite contaminants from the urease preparations introduce artifacts into metabolite profiles, thus leading to misinterpretation.
PLoS One published new progress about Amines Role: ANT (Analyte), BSU (Biological Study, Unclassified), ANST (Analytical Study), BIOL (Biological Study). 64519-82-0 belongs to class alcohols-buliding-blocks, name is (3R,4R,5R)-6-(((2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)hexane-1,2,3,4,5-pentaol, and the molecular formula is C12H24O11, SDS of cas: 64519-82-0.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts