Kolmar, Johannes F. published the artcileImproving Product Specificity of Whole-Cell Alkane Oxidation in Nonconventional Media: A Multivariate Analysis Approach, COA of Formula: C8H18O, the main research area is Tergitol octanol; alkane monooxygenases; high-throughput; multivariate data analyses; nonconventional media; whole-cell bio-oxidation.
Two-liquid-phase reaction media have long been used in bioconversions to supply or remove hydrophobic organic reaction substrates and products to reduce inhibitory and toxic effects on biocatalysts. In case of the terminal oxyfunctionalization of linear alkanes by the AlkBGT monooxygenase the excess alkane substrate is often used as a second phase to extract the alc., aldehyde, and acid products. However, the selection of other carrier phases or surfactants is complex due to a large number of parameters that are involved, such as biocompatibility, substrate bioavailability, and product extraction selectivity. This study combines systematic high-throughput screening with chemometrics to correlate physicochem. parameters of a range of cosolvents to product specificity and yield using a multivariate regression model. Partial least-squares regression shows that the defining factor for product specificity is the solubility properties of the reaction substrate and product in the cosolvent, as measured by Hansen solubility parameters. Thus the polarity of cosolvents determines the accumulation of either alc. or acid products. Whereas usually the acid product accumulates during the reaction, by choosing a more polar cosolvent the 1-alc. product can be accumulated. Especially with Tergitol as a cosolvent, a 3.2-fold improvement in the 1-octanol yield to 18.3 mmol L-1 is achieved relative to the control reaction without cosolvents.
Biotechnology Journal published new progress about Statistical optimization. 111-87-5 belongs to class alcohols-buliding-blocks, name is n-Octanol, and the molecular formula is C8H18O, COA of Formula: C8H18O.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts