Sawyer, Dennis E. published the artcileThe use of an in vitro sperm activation assay to detect chemically induced damage of human sperm nuclei, Name: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Reproductive Toxicology (1995), 9(4), 351-7, database is CAplus and MEDLINE.
We report that human sperm chem. damaged in vitro by treatment with a reversible crosslinker, ethylene glycol bis(sulfosuccinimidylsuccinate; SEGS), display abnormal chromatin decondensation when analyzed in the human sperm activation assay (HSAA). Less than 20% of SEGS-treated sperm fully decondensed, vs. 97% of control sperm. Chem. reversal of the crosslinks by treatment with 5 μM hydroxylamine restored full decondensation in 76% of treated sperm. These results demonstrate that chem. damaged sperm respond abnormally in the HSAA, and that chem. damage to sperm nuclei can be detected using the HSAA. Thus, there is potential for the HSAA to be used to detect chem. alterations of sperm nuclei from men exposed to environmental toxicants.
Reproductive Toxicology published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Name: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.
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