Cleeter, Michael W. J. published the artcileChemical crosslinking of mitochondrial NADH dehydrogenase from bovine heart, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, the publication is Biochemical Journal (1985), 227(2), 467-74, database is CAplus and MEDLINE.
The structure of bovine heart mitochondrial NADH dehydrogenase was investigated by using 2 cleavable crosslinking agents, disuccinimidyl tartrate and (ethylene glycol)yl bis(succinimidyl succinate). Crosslinking was analyzed primarily by immunoblotting to detect products containing subunits of the Fe-protein fraction from chaotropic resolution of the enzyme, namely those of 75, 49, 30, and 13 kilodaltons. Crosslinks were identified between these 4 subunits, from these subunits to the largest subunit of the flavoprotein fraction (which contains the active site for NADH), and from these subunits to polypeptides in the hydrophobic shell (which surrounds the hydrophilic Fe-protein and flavoprotein fractions).
Biochemical Journal published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C18H20N2O12, Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate.
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