functional characterization of HigBA toxin-antitoxin system in Arctic bacterium, Bosea sp. PAMC 26642 was written by Choi, Eunsil;Huh, Ahhyun;Oh, Changmin;Oh, Jeong-Il;Kang, Ho Young;Hwang, Jihwan. And the article was included in Journal of Microbiology (Seoul, Republic of Korea) in 2022.Formula: C9H18O5S The following contents are mentioned in the article:
Toxin-antitoxin (TA) systems are growth-controlling geneticelements consisting of an intracellular toxin protein and itscognate antitoxin. TA systems have been spread among microbial genomes through horizontal gene transfer and arenow prevalent in most bacterial and archaeal genomes. Undernormal growth conditions, antitoxins tightly counteract theactivity of the toxins. Upon stresses, antitoxins are inactivated,releasing activated toxins, which induce growth arrest or celldeath. In this study, among nine functional TA modules in Bosea sp. PAMC 26642 living in Arctic lichen, we investigatedthe functionality of BoHigBA2. BohigBA2 is located close toa genomic island and adjacent to flagellar gene clusters. Theexpression of BohigB2 induced the inhibition of E. coli growthat 37°C, which was more manifest at 18°C, and this growthdefect was reversed when BohigA2 was co-expressed, suggesting that this BoHigBA2 module might be an active TAmodule in Bosea sp. PAMC 26642. Live/dead staining andviable count analyses revealed that the BoHigB2 toxin hada bactericidal effect, causing cell death. Furthermore, we demonstrated that BoHigB2 possessed mRNA-specific RNase activity on various mRNAs and cleaved only mRNAsbeing translated, which might impede overall translation andconsequently lead to cell death. Our study provides the insightto understand the cold adaptation of Bosea sp. PAMC 26642living in the Arctic. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Formula: C9H18O5S).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Formula: C9H18O5S
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