Transiently gene-modulated cell reporter for ultrasensitive detection of estrogen-like compounds in tap water was written by Ren, Yun;Zheng, Jing;Wang, Hailin. And the article was included in Chemosphere in 2022.Name: 4,4′-Methylenediphenol The following contents are mentioned in the article:
Abnormal elevation of indispensable steroid hormone estrogens and exposure to exogenous estrogen-like compounds pose adverse health effects to aquatic animals and human alike. These compounds generally display functionally important estrogenic activity even at extremely low picomolar concentrations In this study we identified one critical but lethal gene (TAF1) that remarkably represses estrogenic activity. This gene is selected as a candidate for genetically modulating an estrogen-responding cell line. To overcome its lethality, instead of adopting a gene knockout strategy, we developed a transient TAF1 depletion strategy using a designed small interfering RNA. By the transient knockdown of TAF1 in the estrogen-responding reporter cell line, the maximum induction signals for endogenous estrogen 17β-estradiol (E2) and environmental estrogens 17α-ethynyl estradiol (EE2) and bisphenol compounds were enhanced by 4.8-13.3 folds. The limit of detection for EE2 is about 8 x 10-15 mol/L. Moreover, by the established method, trace estrogenic activity (14.7-24.2 pg E2 equiv (E2Eq)/L) can be detected in a portion of Tap water samples. This study involved multiple reactions and reactants, such as 4,4′-Methylenediphenol (cas: 620-92-8Name: 4,4′-Methylenediphenol).
4,4′-Methylenediphenol (cas: 620-92-8) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Name: 4,4′-Methylenediphenol
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts