Activity of Na+/K+-ATPase in model lipid membrane at air-water interface was written by Matyszewska, Dorota;Zatloukalova, Martina;Bilewicz, Renata. And the article was included in Electrochimica Acta in 2019.Formula: C37H74NO8P The following contents are mentioned in the article:
The Na+/K+-ATPase pump was reconstituted in proteoliposomes composed of DPPC:DPPE, which were then spread at the air-H2O interface using Langmuir technique. In the presence of the enzyme in the liposomes the resulting lipidic layer at the air-H2O interface became more liquid The effect of Na+/K+-ATPase on the morphol. of Langmuir lipidic layers was monitored by Brewster angle microscopy, which showed the formation of lattice-like structure in between round-shaped lipid domains. The presence of protein stabilized the DPPC:DPPE mixed monolayer at the air-H2O interface, which was revealed by surface pressure measurements in time and ascribed to H bond network formation between the protein and the lipids. The activity of the protein embedded in the lipidic Langmuir layer was measured using spectroscopy and voltammetry. Free phosphate released from ATP reacted with ammonium molybdate and the blue α-Keggin anion formed was detected spectrophotometrically at a wavelength of 710 nm. The results based on spectroscopic assay were complemented with electrochem. methods. The activity of the enzyme could by switched off using the inhibitor – ouabain. The Na+/K+-ATPase activity (2.62 nmol mg-1 min-1) was similar to the activity of the protein solubilized using detergents (3.21 nmol mg-1 min-1). The slightly lower activity was ascribed to the defined orientation of the embedded protein mols. with respect to the air-H2O interface needed for its activity at the air-H2O interface. This study involved multiple reactions and reactants, such as (2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5Formula: C37H74NO8P).
(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl dipalmitate (cas: 923-61-5) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.Formula: C37H74NO8P
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts