Molecular and immunochemical characterization of profilin as major allergen from Platanus acerifolia pollen was written by Yang, Yong-Shi;Xu, Zhi-Qiang;Zhu, Wei;Zhu, Dan-Xuan;Jiao, Yong-Xin;Zhang, Li-Shan;Hou, Yi-Bo;Wei, Ji-Fu;Sun, Jin-Lyu. And the article was included in International Immunopharmacology in 2022.SDS of cas: 367-93-1 The following contents are mentioned in the article:
The Platanus acerifolia (P. acerifolia) pollen is one of the most common causes of allergic respiratory symptoms in China. However, the allergenic components in P. acerifolia are not fully studied yet. The study aimed to determine the mol. and immunochem. characterization of the profilin from P. acerifolia pollen. The coding sequence of profilin was amplified, cloned, and then expressed in Escherichia coli BL21 cells and purified by nickel affinity chromatog. Protein refolding was followed by structural characterization and homol. 3D model building. The allergenicity and cross-reactivity were assessed by ELISA, immunoblotting, or basophil activation test (BAT) using the sera of P. acerifolia allergic patients. The cDNA sequence of profilin was cloned with a 396 bp open reading frame coding for 131 amino acids. The mol. weight of the profilin was approx. 14 kDa, and the predicted structure consisted of 3 α-helixes and 7 β-sheets. Physicochem. anal. indicated the profilin was a stable, relatively thermostable, and relatively conserved protein. The allergenicity determined by ELISA, western blot, and BAT suggested 76.9% (30/39) of the P. acerifolia pollen allergic patients displayed specific IgE recognition of the profilin. The profilin shared > 80% sequence identity with Pop n 2, the profilin from Populus nigra, and observed a significant cross-reactivity with Pop n 2 in IgE-inhibition assay. Profilin, as one of the major component allergens in P. acerifolia pollen, was identified and characterized at mol. and immunochem. levels in this study. These findings would contribute to developing diagnostic and therapeutic strategies for P. acerifolia pollen allergic patients. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1SDS of cas: 367-93-1).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Converting an alcohol to an alkene requires removal of the hydroxyl group and a hydrogen atom on the neighbouring carbon atom. Dehydrations are most commonly carried out by warming the alcohol in the presence of a strong dehydrating acid, such as concentrated sulfuric acid.SDS of cas: 367-93-1
Referemce:
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Alcohols – Chemistry LibreTexts