Multimodal Identification by Transcriptomics and Multiscale Bioassays of Active Components in Xuanfeibaidu Formula to Suppress Macrophage-Mediated Immune Response was written by Zhao, Lu;Liu, Hao;Wang, Yingchao;Wang, Shufang;Xun, Dejin;Wang, Yi;Cheng, Yiyu;Zhang, Boli. And the article was included in Engineering (Beijing, China).Electric Literature of C20H22O8 The following contents are mentioned in the article:
Xuanfeibaidu Formula (XFBD) is a Chinese medicine used in the clin. treatment of coronavirus disease 2019 (COVID-19) patients. Although XFBD has exhibited significant therapeutic efficacy in clin. practice, its underlying pharmacol. mechanism remains unclear. Combination of a comprehensive research approach that includes network pharmacol., transcriptomics, and bioassays in multiple model systems to investigate the pharmacol. mechanism of XFBD and its bioactive substances. High-resolution mass spectrometry was combined with mol. networking to profile the major active substances in XFBD. A total of 104 compounds were identified or tentatively characterized, including flavonoids, terpenes, carboxylic acids, and other types of constituents. Based on the chem. composition of XFBD, a network pharmacol.-based anal. identified inflammation-related pathways as primary targets. Thus, the anti-inflammation activity of XFBD in a lipopolysaccharide-induced acute inflammation mice model was examined XFBD significantly alleviated pulmonary inflammation and decreased the level of serum proinflammatory cytokines. Transcriptomic profiling suggested that genes related to macrophage function were differently expressed after XFBD treatment. Consequently, the effects of XFBD on macrophage activation and mobilization were investigated in a macrophage cell line and a zebrafish wounding model. XFBD exerts strong inhibitory effects on both macrophage activation and migration. Moreover, through multimodal screening, the major components and compounds from the different herbs of XFBD that mediate its anti-inflammation function were further identified. Active components from XFBD, including Polygoni cuspidati Rhizoma, Phragmitis Rhizoma, and Citri grandis Exocarpium rubrum, were then found to strongly downregulate macrophage activation, and polydatin, isoliquiritin, and acteoside were identified as active compounds Components of Artemisiae annuae Herba and Ephedrae Herba were found to substantially inhibit endogenous macrophage migration, while the presence of ephedrine, atractylenolide I, and kaempferol was attributed to these effects. In summary, our study explores the pharmacol. mechanism and effective components of XFBD in inflammation regulation via multimodal approaches, and thereby provides a biol. illustration of the clin. efficacy of XFBD. This study involved multiple reactions and reactants, such as (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6Electric Literature of C20H22O8).
(2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Electric Literature of C20H22O8
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